Experiment: RCH2_defined_noCarbon with Mixed culture; Ralstonia sp. UNC404CL21Col 3:1 starting OD to Klebsiella and Glu_DLac_Acetate_mix 1 x
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS16045 and BWI76_RS16050 are separated by 2 nucleotides BWI76_RS16050 and BWI76_RS16055 overlap by 4 nucleotides BWI76_RS16055 and BWI76_RS16060 overlap by 11 nucleotides
BWI76_RS16045: BWI76_RS16045 - transporter, at 3,326,687 to 3,327,646
_RS16045
BWI76_RS16050: BWI76_RS16050 - malonate decarboxylase holo-ACP synthase, at 3,327,649 to 3,328,269
_RS16050
BWI76_RS16055: BWI76_RS16055 - malonate decarboxylase subunit epsilon, at 3,328,266 to 3,329,168
_RS16055
BWI76_RS16060: BWI76_RS16060 - LysR family transcriptional regulator, at 3,329,158 to 3,330,084
_RS16060
Position (kb)
3327
3328
3329 Strain fitness (log2 ratio)
-2
-1
0
1 at 3326.841 kb on - strand, within BWI76_RS16045 at 3327.330 kb on + strand, within BWI76_RS16045 at 3327.471 kb on + strand, within BWI76_RS16045 at 3327.735 kb on + strand, within BWI76_RS16050 at 3327.812 kb on - strand, within BWI76_RS16050 at 3327.883 kb on - strand, within BWI76_RS16050 at 3328.433 kb on - strand, within BWI76_RS16055 at 3328.607 kb on + strand, within BWI76_RS16055 at 3328.703 kb on + strand, within BWI76_RS16055 at 3328.905 kb on + strand, within BWI76_RS16055 at 3328.913 kb on - strand, within BWI76_RS16055 at 3328.942 kb on - strand, within BWI76_RS16055
Per-strain Table
Position Strand Gene LocusTag Fraction RCH2_defined_noCarbon with Mixed culture; Ralstonia sp. UNC404CL21Col 3:1 starting OD to Klebsiella and Glu_DLac_Acetate_mix 1 x remove 3,326,841 - BWI76_RS16045 0.16 -0.8 3,327,330 + BWI76_RS16045 0.67 -2.0 3,327,471 + BWI76_RS16045 0.82 +1.0 3,327,735 + BWI76_RS16050 0.14 +0.6 3,327,812 - BWI76_RS16050 0.26 +1.0 3,327,883 - BWI76_RS16050 0.38 +0.1 3,328,433 - BWI76_RS16055 0.18 +0.6 3,328,607 + BWI76_RS16055 0.38 +0.9 3,328,703 + BWI76_RS16055 0.48 +1.5 3,328,905 + BWI76_RS16055 0.71 +0.5 3,328,913 - BWI76_RS16055 0.72 +1.5 3,328,942 - BWI76_RS16055 0.75 -0.4
Or see this region's nucleotide sequence