Experiment: RCH2_defined_noCarbon with Mixed culture; Ralstonia sp. UNC404CL21Col 3:1 starting OD to Klebsiella and Glu_DLac_Acetate_mix 1 x
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS15080 and BWI76_RS15085 are separated by 458 nucleotides BWI76_RS15085 and BWI76_RS15090 overlap by 11 nucleotides BWI76_RS15090 and BWI76_RS15095 are separated by 18 nucleotides
BWI76_RS15080: BWI76_RS15080 - hypothetical protein, at 3,158,035 to 3,159,555
_RS15080
BWI76_RS15085: BWI76_RS15085 - 3-deoxy-D-arabinose-heptulosonic-7-phosphate synthase, at 3,160,014 to 3,161,183
_RS15085
BWI76_RS15090: BWI76_RS15090 - 2,3-dihydro-2,3-dihydroxybenzoate dehydrogenase, at 3,161,173 to 3,161,958
_RS15090
BWI76_RS15095: BWI76_RS15095 - isochorismatase, at 3,161,977 to 3,162,609
_RS15095
Position (kb)
3160
3161
3162 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2
3 at 3159.080 kb on - strand, within BWI76_RS15080 at 3159.080 kb on - strand, within BWI76_RS15080 at 3159.080 kb on - strand, within BWI76_RS15080 at 3159.091 kb on - strand, within BWI76_RS15080 at 3159.177 kb on - strand, within BWI76_RS15080 at 3159.183 kb on - strand, within BWI76_RS15080 at 3159.188 kb on - strand, within BWI76_RS15080 at 3159.230 kb on - strand, within BWI76_RS15080 at 3159.250 kb on - strand, within BWI76_RS15080 at 3159.250 kb on - strand, within BWI76_RS15080 at 3159.272 kb on - strand, within BWI76_RS15080 at 3159.272 kb on - strand, within BWI76_RS15080 at 3159.272 kb on - strand, within BWI76_RS15080 at 3159.272 kb on - strand, within BWI76_RS15080 at 3159.272 kb on - strand, within BWI76_RS15080 at 3159.272 kb on - strand, within BWI76_RS15080 at 3159.272 kb on - strand, within BWI76_RS15080 at 3159.274 kb on + strand, within BWI76_RS15080 at 3159.289 kb on + strand, within BWI76_RS15080 at 3159.289 kb on + strand, within BWI76_RS15080 at 3159.289 kb on + strand, within BWI76_RS15080 at 3159.297 kb on - strand, within BWI76_RS15080 at 3159.297 kb on - strand, within BWI76_RS15080 at 3159.297 kb on - strand, within BWI76_RS15080 at 3159.297 kb on - strand, within BWI76_RS15080 at 3159.297 kb on - strand, within BWI76_RS15080 at 3159.297 kb on - strand, within BWI76_RS15080 at 3159.297 kb on - strand, within BWI76_RS15080 at 3159.297 kb on - strand, within BWI76_RS15080 at 3159.297 kb on - strand, within BWI76_RS15080 at 3159.297 kb on - strand, within BWI76_RS15080 at 3159.297 kb on - strand, within BWI76_RS15080 at 3159.297 kb on - strand, within BWI76_RS15080 at 3159.297 kb on - strand, within BWI76_RS15080 at 3159.297 kb on - strand, within BWI76_RS15080 at 3159.297 kb on - strand, within BWI76_RS15080 at 3159.297 kb on - strand, within BWI76_RS15080 at 3159.297 kb on - strand, within BWI76_RS15080 at 3159.297 kb on - strand, within BWI76_RS15080 at 3159.297 kb on - strand, within BWI76_RS15080 at 3159.297 kb on - strand, within BWI76_RS15080 at 3159.297 kb on - strand, within BWI76_RS15080 at 3159.297 kb on - strand, within BWI76_RS15080 at 3159.299 kb on - strand, within BWI76_RS15080 at 3159.299 kb on - strand, within BWI76_RS15080 at 3159.299 kb on - strand, within BWI76_RS15080 at 3159.299 kb on - strand, within BWI76_RS15080 at 3159.313 kb on - strand, within BWI76_RS15080 at 3159.344 kb on - strand, within BWI76_RS15080 at 3159.409 kb on - strand at 3159.410 kb on - strand at 3159.411 kb on + strand at 3159.419 kb on - strand at 3159.419 kb on - strand at 3159.419 kb on - strand at 3159.419 kb on - strand at 3159.419 kb on - strand at 3159.419 kb on - strand at 3159.419 kb on - strand at 3159.419 kb on - strand at 3159.419 kb on - strand at 3159.419 kb on - strand at 3159.421 kb on - strand at 3159.429 kb on - strand at 3159.429 kb on - strand at 3159.476 kb on - strand at 3159.476 kb on - strand at 3159.476 kb on - strand at 3159.529 kb on - strand at 3159.529 kb on - strand at 3159.543 kb on - strand at 3159.543 kb on - strand at 3159.570 kb on - strand at 3159.570 kb on - strand at 3159.590 kb on + strand at 3159.598 kb on - strand at 3159.598 kb on - strand at 3159.598 kb on - strand at 3159.648 kb on - strand at 3159.654 kb on - strand at 3159.686 kb on + strand at 3159.714 kb on - strand at 3159.717 kb on + strand at 3159.735 kb on - strand at 3159.913 kb on + strand at 3159.913 kb on + strand at 3159.914 kb on + strand at 3159.931 kb on - strand at 3159.939 kb on + strand at 3160.045 kb on - strand at 3160.053 kb on - strand at 3160.058 kb on - strand at 3160.058 kb on - strand at 3160.063 kb on - strand at 3160.070 kb on + strand at 3160.070 kb on + strand at 3160.075 kb on + strand at 3160.077 kb on - strand at 3160.078 kb on - strand at 3160.078 kb on - strand at 3160.078 kb on - strand at 3160.078 kb on - strand at 3160.078 kb on - strand at 3160.078 kb on - strand at 3160.078 kb on - strand at 3160.078 kb on - strand at 3160.083 kb on - strand at 3160.083 kb on - strand at 3160.083 kb on - strand at 3160.083 kb on - strand at 3160.085 kb on + strand at 3160.085 kb on + strand at 3160.093 kb on - strand at 3160.095 kb on + strand at 3160.103 kb on - strand at 3160.103 kb on - strand at 3160.107 kb on + strand at 3160.115 kb on - strand at 3160.160 kb on - strand, within BWI76_RS15085 at 3160.176 kb on - strand, within BWI76_RS15085 at 3160.222 kb on + strand, within BWI76_RS15085 at 3160.230 kb on - strand, within BWI76_RS15085 at 3160.258 kb on + strand, within BWI76_RS15085 at 3160.296 kb on - strand at 3160.358 kb on + strand, within BWI76_RS15085 at 3160.373 kb on + strand, within BWI76_RS15085 at 3160.386 kb on - strand, within BWI76_RS15085 at 3160.387 kb on - strand, within BWI76_RS15085 at 3160.391 kb on - strand, within BWI76_RS15085 at 3160.463 kb on + strand, within BWI76_RS15085 at 3160.477 kb on - strand, within BWI76_RS15085 at 3160.552 kb on + strand, within BWI76_RS15085 at 3160.677 kb on + strand, within BWI76_RS15085 at 3160.722 kb on + strand, within BWI76_RS15085 at 3160.785 kb on + strand, within BWI76_RS15085 at 3160.850 kb on + strand, within BWI76_RS15085 at 3160.889 kb on - strand at 3160.891 kb on - strand, within BWI76_RS15085 at 3160.981 kb on + strand, within BWI76_RS15085 at 3161.145 kb on - strand at 3161.190 kb on + strand at 3161.200 kb on - strand at 3161.270 kb on - strand, within BWI76_RS15090 at 3161.275 kb on + strand, within BWI76_RS15090 at 3161.314 kb on - strand, within BWI76_RS15090 at 3161.622 kb on + strand, within BWI76_RS15090 at 3161.719 kb on - strand, within BWI76_RS15090 at 3161.876 kb on + strand, within BWI76_RS15090 at 3161.884 kb on - strand at 3161.975 kb on - strand at 3161.991 kb on + strand at 3162.061 kb on - strand, within BWI76_RS15095 at 3162.120 kb on - strand at 3162.120 kb on - strand, within BWI76_RS15095
Per-strain Table
Position Strand Gene LocusTag Fraction RCH2_defined_noCarbon with Mixed culture; Ralstonia sp. UNC404CL21Col 3:1 starting OD to Klebsiella and Glu_DLac_Acetate_mix 1 x remove 3,159,080 - BWI76_RS15080 0.69 +1.3 3,159,080 - BWI76_RS15080 0.69 +0.1 3,159,080 - BWI76_RS15080 0.69 -0.5 3,159,091 - BWI76_RS15080 0.69 -1.9 3,159,177 - BWI76_RS15080 0.75 +1.0 3,159,183 - BWI76_RS15080 0.75 +0.9 3,159,188 - BWI76_RS15080 0.76 +0.1 3,159,230 - BWI76_RS15080 0.79 +0.2 3,159,250 - BWI76_RS15080 0.80 +0.4 3,159,250 - BWI76_RS15080 0.80 +1.9 3,159,272 - BWI76_RS15080 0.81 +0.1 3,159,272 - BWI76_RS15080 0.81 -1.1 3,159,272 - BWI76_RS15080 0.81 +0.3 3,159,272 - BWI76_RS15080 0.81 +0.4 3,159,272 - BWI76_RS15080 0.81 +0.3 3,159,272 - BWI76_RS15080 0.81 +0.3 3,159,272 - BWI76_RS15080 0.81 +0.0 3,159,274 + BWI76_RS15080 0.81 -0.1 3,159,289 + BWI76_RS15080 0.82 +0.3 3,159,289 + BWI76_RS15080 0.82 -0.9 3,159,289 + BWI76_RS15080 0.82 +0.3 3,159,297 - BWI76_RS15080 0.83 +1.0 3,159,297 - BWI76_RS15080 0.83 +2.4 3,159,297 - BWI76_RS15080 0.83 -1.7 3,159,297 - BWI76_RS15080 0.83 +1.0 3,159,297 - BWI76_RS15080 0.83 -2.9 3,159,297 - BWI76_RS15080 0.83 +1.4 3,159,297 - BWI76_RS15080 0.83 +0.9 3,159,297 - BWI76_RS15080 0.83 +1.1 3,159,297 - BWI76_RS15080 0.83 +1.2 3,159,297 - BWI76_RS15080 0.83 +0.4 3,159,297 - BWI76_RS15080 0.83 +0.0 3,159,297 - BWI76_RS15080 0.83 +0.5 3,159,297 - BWI76_RS15080 0.83 +0.7 3,159,297 - BWI76_RS15080 0.83 -0.0 3,159,297 - BWI76_RS15080 0.83 +1.2 3,159,297 - BWI76_RS15080 0.83 +1.5 3,159,297 - BWI76_RS15080 0.83 +0.3 3,159,297 - BWI76_RS15080 0.83 +0.6 3,159,297 - BWI76_RS15080 0.83 +1.7 3,159,297 - BWI76_RS15080 0.83 +1.6 3,159,297 - BWI76_RS15080 0.83 +0.9 3,159,297 - BWI76_RS15080 0.83 +0.7 3,159,299 - BWI76_RS15080 0.83 +0.8 3,159,299 - BWI76_RS15080 0.83 -0.5 3,159,299 - BWI76_RS15080 0.83 -0.8 3,159,299 - BWI76_RS15080 0.83 -2.4 3,159,313 - BWI76_RS15080 0.84 -2.8 3,159,344 - BWI76_RS15080 0.86 +1.5 3,159,409 - +1.8 3,159,410 - -0.7 3,159,411 + +0.2 3,159,419 - -0.9 3,159,419 - -0.4 3,159,419 - -0.2 3,159,419 - +0.2 3,159,419 - +1.9 3,159,419 - -1.4 3,159,419 - -1.9 3,159,419 - -0.1 3,159,419 - +2.1 3,159,419 - +0.8 3,159,421 - +0.2 3,159,429 - +1.2 3,159,429 - +1.0 3,159,476 - +1.2 3,159,476 - +0.9 3,159,476 - +0.9 3,159,529 - +1.8 3,159,529 - -0.9 3,159,543 - +0.6 3,159,543 - +0.6 3,159,570 - +1.2 3,159,570 - +1.2 3,159,590 + +1.2 3,159,598 - -0.0 3,159,598 - -1.1 3,159,598 - -0.4 3,159,648 - -4.3 3,159,654 - +1.6 3,159,686 + -1.4 3,159,714 - +0.9 3,159,717 + -0.6 3,159,735 - -2.5 3,159,913 + +0.1 3,159,913 + -1.6 3,159,914 + +2.3 3,159,931 - -2.9 3,159,939 + -1.7 3,160,045 - -1.1 3,160,053 - +0.7 3,160,058 - -2.4 3,160,058 - -0.2 3,160,063 - +1.3 3,160,070 + +1.1 3,160,070 + -2.1 3,160,075 + -1.1 3,160,077 - -2.1 3,160,078 - +0.9 3,160,078 - +0.9 3,160,078 - -0.5 3,160,078 - -1.6 3,160,078 - +0.9 3,160,078 - -0.3 3,160,078 - -1.5 3,160,078 - -2.4 3,160,083 - +1.2 3,160,083 - -1.4 3,160,083 - +0.9 3,160,083 - +0.1 3,160,085 + +2.4 3,160,085 + -1.9 3,160,093 - +0.8 3,160,095 + -2.1 3,160,103 - -0.0 3,160,103 - -2.1 3,160,107 + -1.2 3,160,115 - -0.6 3,160,160 - BWI76_RS15085 0.12 -2.1 3,160,176 - BWI76_RS15085 0.14 -3.5 3,160,222 + BWI76_RS15085 0.18 +2.9 3,160,230 - BWI76_RS15085 0.18 -0.2 3,160,258 + BWI76_RS15085 0.21 -2.4 3,160,296 - +0.9 3,160,358 + BWI76_RS15085 0.29 -0.6 3,160,373 + BWI76_RS15085 0.31 +0.9 3,160,386 - BWI76_RS15085 0.32 -2.6 3,160,387 - BWI76_RS15085 0.32 +1.6 3,160,391 - BWI76_RS15085 0.32 -2.8 3,160,463 + BWI76_RS15085 0.38 -1.6 3,160,477 - BWI76_RS15085 0.40 +1.3 3,160,552 + BWI76_RS15085 0.46 -2.5 3,160,677 + BWI76_RS15085 0.57 +0.9 3,160,722 + BWI76_RS15085 0.61 +0.0 3,160,785 + BWI76_RS15085 0.66 +0.7 3,160,850 + BWI76_RS15085 0.71 +1.3 3,160,889 - +3.5 3,160,891 - BWI76_RS15085 0.75 +0.9 3,160,981 + BWI76_RS15085 0.83 +0.8 3,161,145 - +1.9 3,161,190 + -0.6 3,161,200 - +0.9 3,161,270 - BWI76_RS15090 0.12 +0.1 3,161,275 + BWI76_RS15090 0.13 +1.1 3,161,314 - BWI76_RS15090 0.18 -0.1 3,161,622 + BWI76_RS15090 0.57 +0.7 3,161,719 - BWI76_RS15090 0.69 +0.1 3,161,876 + BWI76_RS15090 0.89 -1.1 3,161,884 - +1.6 3,161,975 - -0.8 3,161,991 + -0.1 3,162,061 - BWI76_RS15095 0.13 +0.9 3,162,120 - -0.1 3,162,120 - BWI76_RS15095 0.23 +0.7
Or see this region's nucleotide sequence