Experiment: RCH2_defined_noCarbon with Mixed culture; Ralstonia sp. UNC404CL21Col 3:1 starting OD to Klebsiella and Glu_DLac_Acetate_mix 1 x
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS14505 and BWI76_RS14510 are separated by 27 nucleotides BWI76_RS14510 and BWI76_RS14515 overlap by 8 nucleotides
BWI76_RS14505: BWI76_RS14505 - salicylate synthase Irp9, at 3,028,508 to 3,029,818
_RS14505
BWI76_RS14510: BWI76_RS14510 - MFS transporter, at 3,029,846 to 3,031,126
_RS14510
BWI76_RS14515: BWI76_RS14515 - yersiniabactin-iron ABC transporter permease ATP-binding protein YbtQ, at 3,031,119 to 3,032,921
_RS14515
Position (kb)
3029
3030
3031
3032 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2 at 3028.983 kb on - strand, within BWI76_RS14505 at 3029.349 kb on - strand, within BWI76_RS14505 at 3029.734 kb on - strand at 3030.343 kb on - strand, within BWI76_RS14510 at 3030.519 kb on + strand, within BWI76_RS14510 at 3030.530 kb on + strand, within BWI76_RS14510 at 3030.653 kb on + strand, within BWI76_RS14510 at 3030.661 kb on - strand, within BWI76_RS14510 at 3030.934 kb on - strand, within BWI76_RS14510 at 3031.050 kb on - strand at 3031.217 kb on - strand at 3031.242 kb on - strand at 3031.512 kb on + strand, within BWI76_RS14515 at 3031.573 kb on - strand, within BWI76_RS14515 at 3032.035 kb on + strand, within BWI76_RS14515
Per-strain Table
Position Strand Gene LocusTag Fraction RCH2_defined_noCarbon with Mixed culture; Ralstonia sp. UNC404CL21Col 3:1 starting OD to Klebsiella and Glu_DLac_Acetate_mix 1 x remove 3,028,983 - BWI76_RS14505 0.36 +1.4 3,029,349 - BWI76_RS14505 0.64 +0.4 3,029,734 - +2.4 3,030,343 - BWI76_RS14510 0.39 -1.4 3,030,519 + BWI76_RS14510 0.53 -2.2 3,030,530 + BWI76_RS14510 0.53 -1.1 3,030,653 + BWI76_RS14510 0.63 +0.0 3,030,661 - BWI76_RS14510 0.64 +0.2 3,030,934 - BWI76_RS14510 0.85 -3.7 3,031,050 - +0.3 3,031,217 - -1.5 3,031,242 - +0.2 3,031,512 + BWI76_RS14515 0.22 -1.6 3,031,573 - BWI76_RS14515 0.25 +0.2 3,032,035 + BWI76_RS14515 0.51 -4.0
Or see this region's nucleotide sequence