Experiment: RCH2_defined_noCarbon with Mixed culture; Ralstonia sp. UNC404CL21Col 3:1 starting OD to Klebsiella and Glu_DLac_Acetate_mix 1 x
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS14385 and BWI76_RS14390 overlap by 17 nucleotides BWI76_RS14390 and BWI76_RS14395 are separated by 517 nucleotides BWI76_RS14395 and BWI76_RS14400 are separated by 110 nucleotides
BWI76_RS14385: BWI76_RS14385 - hypothetical protein, at 3,005,453 to 3,007,003
_RS14385
BWI76_RS14390: BWI76_RS14390 - hypothetical protein, at 3,006,987 to 3,007,862
_RS14390
BWI76_RS14395: BWI76_RS14395 - hypothetical protein, at 3,008,380 to 3,008,517
_RS14395
BWI76_RS14400: BWI76_RS14400 - ISNCY family transposase, at 3,008,628 to 3,009,198
_RS14400
Position (kb)
3006
3007
3008 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2 at 3005.998 kb on + strand, within BWI76_RS14385 at 3006.006 kb on - strand, within BWI76_RS14385 at 3006.146 kb on - strand, within BWI76_RS14385 at 3006.164 kb on + strand at 3006.242 kb on + strand, within BWI76_RS14385 at 3006.350 kb on - strand, within BWI76_RS14385 at 3006.356 kb on - strand, within BWI76_RS14385 at 3006.366 kb on - strand, within BWI76_RS14385 at 3006.403 kb on - strand, within BWI76_RS14385 at 3006.480 kb on - strand, within BWI76_RS14385 at 3006.571 kb on + strand, within BWI76_RS14385 at 3006.658 kb on + strand, within BWI76_RS14385 at 3006.792 kb on + strand, within BWI76_RS14385 at 3006.799 kb on - strand, within BWI76_RS14385 at 3007.025 kb on + strand at 3007.224 kb on - strand at 3007.224 kb on - strand, within BWI76_RS14390 at 3007.224 kb on - strand, within BWI76_RS14390 at 3007.860 kb on + strand at 3008.043 kb on + strand at 3008.043 kb on + strand at 3008.045 kb on + strand at 3008.045 kb on + strand at 3008.077 kb on - strand at 3008.160 kb on - strand at 3008.247 kb on + strand at 3008.260 kb on + strand at 3008.260 kb on + strand at 3008.605 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction RCH2_defined_noCarbon with Mixed culture; Ralstonia sp. UNC404CL21Col 3:1 starting OD to Klebsiella and Glu_DLac_Acetate_mix 1 x remove 3,005,998 + BWI76_RS14385 0.35 -3.9 3,006,006 - BWI76_RS14385 0.36 -3.1 3,006,146 - BWI76_RS14385 0.45 +0.9 3,006,164 + +0.9 3,006,242 + BWI76_RS14385 0.51 -1.5 3,006,350 - BWI76_RS14385 0.58 -0.5 3,006,356 - BWI76_RS14385 0.58 +0.8 3,006,366 - BWI76_RS14385 0.59 +1.9 3,006,403 - BWI76_RS14385 0.61 -1.1 3,006,480 - BWI76_RS14385 0.66 +0.9 3,006,571 + BWI76_RS14385 0.72 -0.1 3,006,658 + BWI76_RS14385 0.78 -3.1 3,006,792 + BWI76_RS14385 0.86 +2.6 3,006,799 - BWI76_RS14385 0.87 +0.9 3,007,025 + -0.7 3,007,224 - -0.7 3,007,224 - BWI76_RS14390 0.27 -3.5 3,007,224 - BWI76_RS14390 0.27 -0.7 3,007,860 + +0.8 3,008,043 + +1.6 3,008,043 + +0.8 3,008,045 + +0.6 3,008,045 + +0.6 3,008,077 - -0.2 3,008,160 - -0.2 3,008,247 + -3.5 3,008,260 + -1.5 3,008,260 + -1.7 3,008,605 - -0.2
Or see this region's nucleotide sequence