Experiment: RCH2_defined_noCarbon with Mixed culture; Ralstonia sp. UNC404CL21Col 3:1 starting OD to Klebsiella and Glu_DLac_Acetate_mix 1 x
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS14085 and BWI76_RS14090 are separated by 123 nucleotides BWI76_RS14090 and BWI76_RS14095 are separated by 30 nucleotides BWI76_RS14095 and BWI76_RS14100 are separated by 272 nucleotides
BWI76_RS14085: BWI76_RS14085 - hypothetical protein, at 2,944,741 to 2,945,163
_RS14085
BWI76_RS14090: BWI76_RS14090 - polyketide cyclase, at 2,945,287 to 2,945,740
_RS14090
BWI76_RS14095: BWI76_RS14095 - hypothetical protein, at 2,945,771 to 2,947,096
_RS14095
BWI76_RS14100: BWI76_RS14100 - diguanylate phosphodiesterase, at 2,947,369 to 2,948,907
_RS14100
Position (kb)
2945
2946
2947
2948 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2
3 at 2945.332 kb on + strand at 2945.407 kb on - strand, within BWI76_RS14090 at 2945.451 kb on - strand, within BWI76_RS14090 at 2945.573 kb on - strand, within BWI76_RS14090 at 2945.963 kb on + strand, within BWI76_RS14095 at 2945.963 kb on + strand, within BWI76_RS14095 at 2945.979 kb on + strand, within BWI76_RS14095 at 2945.997 kb on - strand, within BWI76_RS14095 at 2946.465 kb on - strand, within BWI76_RS14095 at 2946.465 kb on - strand, within BWI76_RS14095 at 2946.503 kb on + strand, within BWI76_RS14095 at 2946.550 kb on + strand, within BWI76_RS14095 at 2946.606 kb on + strand, within BWI76_RS14095 at 2946.663 kb on - strand, within BWI76_RS14095 at 2946.747 kb on + strand, within BWI76_RS14095 at 2946.824 kb on - strand, within BWI76_RS14095 at 2946.829 kb on + strand, within BWI76_RS14095 at 2946.835 kb on - strand, within BWI76_RS14095 at 2947.072 kb on - strand at 2947.083 kb on - strand at 2947.129 kb on - strand at 2947.280 kb on + strand at 2947.288 kb on - strand at 2947.288 kb on - strand at 2947.367 kb on - strand at 2947.423 kb on + strand at 2947.444 kb on - strand at 2947.503 kb on + strand at 2947.514 kb on + strand at 2947.514 kb on + strand at 2947.514 kb on + strand at 2947.514 kb on + strand at 2947.522 kb on - strand at 2947.522 kb on - strand at 2947.522 kb on - strand at 2947.522 kb on - strand at 2947.548 kb on - strand, within BWI76_RS14100 at 2947.564 kb on - strand, within BWI76_RS14100 at 2947.569 kb on - strand, within BWI76_RS14100 at 2947.603 kb on + strand, within BWI76_RS14100 at 2947.973 kb on - strand, within BWI76_RS14100 at 2947.995 kb on - strand, within BWI76_RS14100 at 2948.068 kb on + strand, within BWI76_RS14100
Per-strain Table
Position Strand Gene LocusTag Fraction RCH2_defined_noCarbon with Mixed culture; Ralstonia sp. UNC404CL21Col 3:1 starting OD to Klebsiella and Glu_DLac_Acetate_mix 1 x remove 2,945,332 + +0.9 2,945,407 - BWI76_RS14090 0.26 -0.5 2,945,451 - BWI76_RS14090 0.36 +0.9 2,945,573 - BWI76_RS14090 0.63 -2.3 2,945,963 + BWI76_RS14095 0.14 -2.5 2,945,963 + BWI76_RS14095 0.14 -1.5 2,945,979 + BWI76_RS14095 0.16 -4.6 2,945,997 - BWI76_RS14095 0.17 +0.9 2,946,465 - BWI76_RS14095 0.52 +0.5 2,946,465 - BWI76_RS14095 0.52 +0.4 2,946,503 + BWI76_RS14095 0.55 +1.0 2,946,550 + BWI76_RS14095 0.59 +0.4 2,946,606 + BWI76_RS14095 0.63 +0.4 2,946,663 - BWI76_RS14095 0.67 -4.6 2,946,747 + BWI76_RS14095 0.74 -1.5 2,946,824 - BWI76_RS14095 0.79 +0.4 2,946,829 + BWI76_RS14095 0.80 +1.7 2,946,835 - BWI76_RS14095 0.80 +1.8 2,947,072 - -0.1 2,947,083 - -2.4 2,947,129 - +0.5 2,947,280 + -2.0 2,947,288 - +0.2 2,947,288 - -0.1 2,947,367 - -1.2 2,947,423 + -1.1 2,947,444 - +0.1 2,947,503 + -0.1 2,947,514 + -0.6 2,947,514 + +0.3 2,947,514 + -0.1 2,947,514 + +3.6 2,947,522 - +0.9 2,947,522 - +0.2 2,947,522 - +1.8 2,947,522 - +0.9 2,947,548 - BWI76_RS14100 0.12 -0.2 2,947,564 - BWI76_RS14100 0.13 +0.6 2,947,569 - BWI76_RS14100 0.13 -1.1 2,947,603 + BWI76_RS14100 0.15 +0.3 2,947,973 - BWI76_RS14100 0.39 -3.6 2,947,995 - BWI76_RS14100 0.41 -2.6 2,948,068 + BWI76_RS14100 0.45 +0.4
Or see this region's nucleotide sequence