Experiment: RCH2_defined_noCarbon with Mixed culture; Ralstonia sp. UNC404CL21Col 3:1 starting OD to Klebsiella and Glu_DLac_Acetate_mix 1 x
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS13840 and BWI76_RS13845 are separated by 114 nucleotides BWI76_RS13845 and BWI76_RS13850 overlap by 1 nucleotides BWI76_RS13850 and BWI76_RS13855 are separated by 79 nucleotides
BWI76_RS13840: BWI76_RS13840 - MBL fold metallo-hydrolase, at 2,887,369 to 2,888,220
_RS13840
BWI76_RS13845: BWI76_RS13845 - LysR family transcriptional regulator, at 2,888,335 to 2,889,237
_RS13845
BWI76_RS13850: BWI76_RS13850 - N-acetyltransferase, at 2,889,237 to 2,889,716
_RS13850
BWI76_RS13855: BWI76_RS13855 - peroxiredoxin, at 2,889,796 to 2,890,227
_RS13855
Position (kb)
2888
2889
2890 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1 at 2887.425 kb on - strand at 2887.853 kb on + strand, within BWI76_RS13840 at 2887.917 kb on - strand, within BWI76_RS13840 at 2888.086 kb on - strand, within BWI76_RS13840 at 2888.096 kb on - strand, within BWI76_RS13840 at 2888.096 kb on - strand, within BWI76_RS13840 at 2888.177 kb on + strand at 2888.177 kb on + strand at 2888.340 kb on - strand at 2888.441 kb on + strand, within BWI76_RS13845 at 2888.468 kb on + strand, within BWI76_RS13845 at 2888.608 kb on - strand, within BWI76_RS13845 at 2888.681 kb on + strand, within BWI76_RS13845 at 2888.820 kb on + strand, within BWI76_RS13845 at 2888.820 kb on + strand, within BWI76_RS13845 at 2889.458 kb on + strand, within BWI76_RS13850 at 2889.509 kb on - strand, within BWI76_RS13850 at 2889.546 kb on + strand, within BWI76_RS13850 at 2889.654 kb on - strand, within BWI76_RS13850 at 2889.718 kb on - strand at 2889.763 kb on - strand at 2889.919 kb on + strand, within BWI76_RS13855 at 2889.989 kb on + strand, within BWI76_RS13855 at 2890.008 kb on + strand, within BWI76_RS13855 at 2890.215 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction RCH2_defined_noCarbon with Mixed culture; Ralstonia sp. UNC404CL21Col 3:1 starting OD to Klebsiella and Glu_DLac_Acetate_mix 1 x remove 2,887,425 - +0.4 2,887,853 + BWI76_RS13840 0.57 -1.6 2,887,917 - BWI76_RS13840 0.64 -2.5 2,888,086 - BWI76_RS13840 0.84 -0.4 2,888,096 - BWI76_RS13840 0.85 +0.6 2,888,096 - BWI76_RS13840 0.85 -0.8 2,888,177 + +0.3 2,888,177 + +0.8 2,888,340 - -4.2 2,888,441 + BWI76_RS13845 0.12 +1.0 2,888,468 + BWI76_RS13845 0.15 +1.1 2,888,608 - BWI76_RS13845 0.30 -0.2 2,888,681 + BWI76_RS13845 0.38 -2.0 2,888,820 + BWI76_RS13845 0.54 +1.2 2,888,820 + BWI76_RS13845 0.54 -4.6 2,889,458 + BWI76_RS13850 0.46 -2.2 2,889,509 - BWI76_RS13850 0.57 +0.4 2,889,546 + BWI76_RS13850 0.64 -0.4 2,889,654 - BWI76_RS13850 0.87 -3.4 2,889,718 - -0.9 2,889,763 - -1.9 2,889,919 + BWI76_RS13855 0.28 -1.4 2,889,989 + BWI76_RS13855 0.45 +0.8 2,890,008 + BWI76_RS13855 0.49 +0.2 2,890,215 + -0.6
Or see this region's nucleotide sequence