Experiment: RCH2_defined_noCarbon with Mixed culture; Ralstonia sp. UNC404CL21Col 3:1 starting OD to Klebsiella and Glu_DLac_Acetate_mix 1 x
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS13280 and BWI76_RS13285 are separated by 172 nucleotides BWI76_RS13285 and BWI76_RS13290 overlap by 4 nucleotides BWI76_RS13290 and BWI76_RS13295 are separated by 239 nucleotides
BWI76_RS13280: BWI76_RS13280 - LysR family transcriptional regulator, at 2,768,905 to 2,769,828
_RS13280
BWI76_RS13285: BWI76_RS13285 - protocatechuate 3,4-dioxygenase subunit alpha, at 2,770,001 to 2,770,621
_RS13285
BWI76_RS13290: BWI76_RS13290 - protocatechuate 3,4-dioxygenase subunit beta, at 2,770,618 to 2,771,358
_RS13290
BWI76_RS13295: BWI76_RS13295 - DUF1338 domain-containing protein, at 2,771,598 to 2,772,941
_RS13295
Position (kb)
2770
2771
2772 Strain fitness (log2 ratio)
-3
-2
-1
0
1 at 2769.700 kb on + strand, within BWI76_RS13280 at 2769.728 kb on - strand, within BWI76_RS13280 at 2769.757 kb on - strand at 2769.783 kb on - strand at 2769.794 kb on + strand at 2770.020 kb on + strand at 2770.256 kb on + strand, within BWI76_RS13285 at 2770.653 kb on + strand at 2770.661 kb on - strand at 2770.888 kb on + strand, within BWI76_RS13290 at 2770.919 kb on - strand, within BWI76_RS13290 at 2771.203 kb on - strand, within BWI76_RS13290 at 2771.475 kb on + strand at 2771.602 kb on - strand at 2771.622 kb on - strand at 2771.689 kb on - strand at 2771.718 kb on - strand at 2771.737 kb on + strand, within BWI76_RS13295 at 2771.759 kb on + strand, within BWI76_RS13295 at 2772.295 kb on + strand, within BWI76_RS13295
Per-strain Table
Position Strand Gene LocusTag Fraction RCH2_defined_noCarbon with Mixed culture; Ralstonia sp. UNC404CL21Col 3:1 starting OD to Klebsiella and Glu_DLac_Acetate_mix 1 x remove 2,769,700 + BWI76_RS13280 0.86 -2.1 2,769,728 - BWI76_RS13280 0.89 -0.4 2,769,757 - -3.2 2,769,783 - +0.4 2,769,794 + +0.5 2,770,020 + -1.6 2,770,256 + BWI76_RS13285 0.41 -3.1 2,770,653 + -0.8 2,770,661 - +1.6 2,770,888 + BWI76_RS13290 0.36 -2.4 2,770,919 - BWI76_RS13290 0.41 +0.7 2,771,203 - BWI76_RS13290 0.79 +0.2 2,771,475 + +0.6 2,771,602 - -0.7 2,771,622 - +0.0 2,771,689 - -1.4 2,771,718 - +1.0 2,771,737 + BWI76_RS13295 0.10 -2.0 2,771,759 + BWI76_RS13295 0.12 +0.0 2,772,295 + BWI76_RS13295 0.52 -0.7
Or see this region's nucleotide sequence