Experiment: RCH2_defined_noCarbon with Mixed culture; Ralstonia sp. UNC404CL21Col 3:1 starting OD to Klebsiella and Glu_DLac_Acetate_mix 1 x
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS13270 and BWI76_RS13275 are separated by 38 nucleotides BWI76_RS13275 and BWI76_RS13280 are separated by 68 nucleotides BWI76_RS13280 and BWI76_RS13285 are separated by 172 nucleotides BWI76_RS13285 and BWI76_RS13290 overlap by 4 nucleotides
BWI76_RS13270: BWI76_RS13270 - membrane-bound PQQ-dependent dehydrogenase, glucose/quinate/shikimate family, at 2,766,240 to 2,768,612
_RS13270
BWI76_RS13275: BWI76_RS13275 - hypothetical protein, at 2,768,651 to 2,768,836
_RS13275
BWI76_RS13280: BWI76_RS13280 - LysR family transcriptional regulator, at 2,768,905 to 2,769,828
_RS13280
BWI76_RS13285: BWI76_RS13285 - protocatechuate 3,4-dioxygenase subunit alpha, at 2,770,001 to 2,770,621
_RS13285
BWI76_RS13290: BWI76_RS13290 - protocatechuate 3,4-dioxygenase subunit beta, at 2,770,618 to 2,771,358
_RS13290
Position (kb)
2768
2769
2770 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1 at 2767.984 kb on - strand, within BWI76_RS13270 at 2768.034 kb on - strand, within BWI76_RS13270 at 2768.044 kb on - strand, within BWI76_RS13270 at 2768.068 kb on - strand, within BWI76_RS13270 at 2768.281 kb on + strand, within BWI76_RS13270 at 2768.573 kb on - strand at 2768.763 kb on + strand, within BWI76_RS13275 at 2769.007 kb on - strand, within BWI76_RS13280 at 2769.080 kb on - strand, within BWI76_RS13280 at 2769.505 kb on - strand, within BWI76_RS13280 at 2769.507 kb on - strand, within BWI76_RS13280 at 2769.574 kb on - strand, within BWI76_RS13280 at 2769.577 kb on - strand, within BWI76_RS13280 at 2769.700 kb on + strand, within BWI76_RS13280 at 2769.728 kb on - strand, within BWI76_RS13280 at 2769.757 kb on - strand at 2769.783 kb on - strand at 2769.794 kb on + strand at 2770.020 kb on + strand at 2770.256 kb on + strand, within BWI76_RS13285 at 2770.653 kb on + strand at 2770.661 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction RCH2_defined_noCarbon with Mixed culture; Ralstonia sp. UNC404CL21Col 3:1 starting OD to Klebsiella and Glu_DLac_Acetate_mix 1 x remove 2,767,984 - BWI76_RS13270 0.73 +0.1 2,768,034 - BWI76_RS13270 0.76 +1.0 2,768,044 - BWI76_RS13270 0.76 +0.5 2,768,068 - BWI76_RS13270 0.77 -0.9 2,768,281 + BWI76_RS13270 0.86 +1.2 2,768,573 - +1.1 2,768,763 + BWI76_RS13275 0.60 -1.5 2,769,007 - BWI76_RS13280 0.11 +0.4 2,769,080 - BWI76_RS13280 0.19 -4.7 2,769,505 - BWI76_RS13280 0.65 +0.5 2,769,507 - BWI76_RS13280 0.65 -0.1 2,769,574 - BWI76_RS13280 0.72 +0.9 2,769,577 - BWI76_RS13280 0.73 -0.1 2,769,700 + BWI76_RS13280 0.86 -2.1 2,769,728 - BWI76_RS13280 0.89 -0.4 2,769,757 - -3.2 2,769,783 - +0.4 2,769,794 + +0.5 2,770,020 + -1.6 2,770,256 + BWI76_RS13285 0.41 -3.1 2,770,653 + -0.8 2,770,661 - +1.6
Or see this region's nucleotide sequence