Experiment: RCH2_defined_noCarbon with Mixed culture; Ralstonia sp. UNC404CL21Col 3:1 starting OD to Klebsiella and Glu_DLac_Acetate_mix 1 x
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS11865 and BWI76_RS11870 are separated by 9 nucleotides BWI76_RS11870 and BWI76_RS11875 are separated by 29 nucleotides BWI76_RS11875 and BWI76_RS11880 are separated by 228 nucleotides
BWI76_RS11865: BWI76_RS11865 - phosphatase, at 2,495,850 to 2,496,719
_RS11865
BWI76_RS11870: BWI76_RS11870 - hypothetical protein, at 2,496,729 to 2,497,349
_RS11870
BWI76_RS11875: BWI76_RS11875 - hypothetical protein, at 2,497,379 to 2,499,277
_RS11875
BWI76_RS11880: BWI76_RS11880 - 23S rRNA pseudouridylate synthase B, at 2,499,506 to 2,500,393
_RS11880
Position (kb)
2497
2498
2499
2500 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 2496.480 kb on - strand, within BWI76_RS11865 at 2496.541 kb on - strand, within BWI76_RS11865 at 2496.588 kb on + strand, within BWI76_RS11865 at 2496.691 kb on + strand at 2496.691 kb on + strand at 2496.699 kb on - strand at 2496.699 kb on - strand at 2496.815 kb on - strand, within BWI76_RS11870 at 2496.927 kb on - strand, within BWI76_RS11870 at 2497.015 kb on + strand, within BWI76_RS11870 at 2497.072 kb on + strand, within BWI76_RS11870 at 2497.083 kb on + strand, within BWI76_RS11870 at 2497.205 kb on + strand, within BWI76_RS11870 at 2497.205 kb on + strand, within BWI76_RS11870 at 2497.423 kb on + strand at 2497.479 kb on + strand at 2497.827 kb on + strand, within BWI76_RS11875 at 2498.121 kb on + strand, within BWI76_RS11875 at 2498.496 kb on - strand, within BWI76_RS11875 at 2498.500 kb on + strand, within BWI76_RS11875 at 2498.530 kb on - strand, within BWI76_RS11875 at 2498.542 kb on + strand, within BWI76_RS11875 at 2498.550 kb on - strand, within BWI76_RS11875 at 2498.989 kb on + strand, within BWI76_RS11875 at 2499.085 kb on - strand, within BWI76_RS11875 at 2499.266 kb on + strand at 2499.491 kb on + strand at 2499.491 kb on + strand at 2499.491 kb on + strand at 2499.499 kb on - strand at 2499.499 kb on - strand at 2500.084 kb on + strand, within BWI76_RS11880 at 2500.130 kb on + strand, within BWI76_RS11880 at 2500.242 kb on - strand, within BWI76_RS11880 at 2500.242 kb on - strand, within BWI76_RS11880 at 2500.242 kb on - strand, within BWI76_RS11880 at 2500.242 kb on - strand, within BWI76_RS11880 at 2500.242 kb on - strand, within BWI76_RS11880 at 2500.257 kb on + strand, within BWI76_RS11880 at 2500.257 kb on + strand, within BWI76_RS11880 at 2500.265 kb on - strand, within BWI76_RS11880
Per-strain Table
Position Strand Gene LocusTag Fraction RCH2_defined_noCarbon with Mixed culture; Ralstonia sp. UNC404CL21Col 3:1 starting OD to Klebsiella and Glu_DLac_Acetate_mix 1 x remove 2,496,480 - BWI76_RS11865 0.72 -1.8 2,496,541 - BWI76_RS11865 0.79 -2.7 2,496,588 + BWI76_RS11865 0.85 +1.4 2,496,691 + +0.6 2,496,691 + +1.0 2,496,699 - -2.4 2,496,699 - -2.4 2,496,815 - BWI76_RS11870 0.14 +0.4 2,496,927 - BWI76_RS11870 0.32 -2.2 2,497,015 + BWI76_RS11870 0.46 +2.0 2,497,072 + BWI76_RS11870 0.55 +0.1 2,497,083 + BWI76_RS11870 0.57 -0.2 2,497,205 + BWI76_RS11870 0.77 +0.9 2,497,205 + BWI76_RS11870 0.77 +0.4 2,497,423 + -1.4 2,497,479 + +0.3 2,497,827 + BWI76_RS11875 0.24 -3.2 2,498,121 + BWI76_RS11875 0.39 +1.2 2,498,496 - BWI76_RS11875 0.59 +0.4 2,498,500 + BWI76_RS11875 0.59 +1.3 2,498,530 - BWI76_RS11875 0.61 +2.0 2,498,542 + BWI76_RS11875 0.61 -2.1 2,498,550 - BWI76_RS11875 0.62 +0.5 2,498,989 + BWI76_RS11875 0.85 +0.4 2,499,085 - BWI76_RS11875 0.90 +1.1 2,499,266 + +0.2 2,499,491 + +1.2 2,499,491 + +0.2 2,499,491 + +1.2 2,499,499 - +0.3 2,499,499 - +0.7 2,500,084 + BWI76_RS11880 0.65 +0.2 2,500,130 + BWI76_RS11880 0.70 -0.3 2,500,242 - BWI76_RS11880 0.83 +0.5 2,500,242 - BWI76_RS11880 0.83 +1.3 2,500,242 - BWI76_RS11880 0.83 +0.0 2,500,242 - BWI76_RS11880 0.83 +0.5 2,500,242 - BWI76_RS11880 0.83 -0.6 2,500,257 + BWI76_RS11880 0.85 +0.8 2,500,257 + BWI76_RS11880 0.85 -0.3 2,500,265 - BWI76_RS11880 0.85 +0.3
Or see this region's nucleotide sequence