Experiment: RCH2_defined_noCarbon with Mixed culture; Ralstonia sp. UNC404CL21Col 3:1 starting OD to Klebsiella and Glu_DLac_Acetate_mix 1 x
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS11280 and BWI76_RS11285 are separated by 84 nucleotides BWI76_RS11285 and BWI76_RS11290 are separated by 100 nucleotides BWI76_RS11290 and BWI76_RS11295 are separated by 87 nucleotides BWI76_RS11295 and BWI76_RS11300 are separated by 82 nucleotides
BWI76_RS11280: BWI76_RS11280 - two-component system response regulator PhoP, at 2,379,284 to 2,379,958
_RS11280
BWI76_RS11285: BWI76_RS11285 - transcriptional regulator, at 2,380,043 to 2,380,489
_RS11285
BWI76_RS11290: BWI76_RS11290 - MFS transporter, at 2,380,590 to 2,381,819
_RS11290
BWI76_RS11295: BWI76_RS11295 - AraC family transcriptional regulator, at 2,381,907 to 2,382,713
_RS11295
BWI76_RS11300: BWI76_RS11300 - cupin, at 2,382,796 to 2,383,158
_RS11300
Position (kb)
2380
2381
2382 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 2379.930 kb on + strand at 2380.386 kb on - strand, within BWI76_RS11285 at 2380.818 kb on + strand, within BWI76_RS11290 at 2380.884 kb on - strand, within BWI76_RS11290 at 2381.047 kb on - strand, within BWI76_RS11290 at 2381.123 kb on - strand, within BWI76_RS11290 at 2381.207 kb on - strand, within BWI76_RS11290 at 2381.207 kb on - strand, within BWI76_RS11290 at 2382.184 kb on - strand, within BWI76_RS11295 at 2382.749 kb on - strand at 2382.780 kb on - strand at 2382.815 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction RCH2_defined_noCarbon with Mixed culture; Ralstonia sp. UNC404CL21Col 3:1 starting OD to Klebsiella and Glu_DLac_Acetate_mix 1 x remove 2,379,930 + -1.2 2,380,386 - BWI76_RS11285 0.77 +0.3 2,380,818 + BWI76_RS11290 0.19 +2.7 2,380,884 - BWI76_RS11290 0.24 -2.1 2,381,047 - BWI76_RS11290 0.37 +1.4 2,381,123 - BWI76_RS11290 0.43 -0.5 2,381,207 - BWI76_RS11290 0.50 -0.0 2,381,207 - BWI76_RS11290 0.50 -0.9 2,382,184 - BWI76_RS11295 0.34 -0.7 2,382,749 - +0.2 2,382,780 - +0.7 2,382,815 - -0.9
Or see this region's nucleotide sequence