Experiment: RCH2_defined_noCarbon with Mixed culture; Ralstonia sp. UNC404CL21Col 3:1 starting OD to Klebsiella and Glu_DLac_Acetate_mix 1 x
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS11185 and BWI76_RS11190 are separated by 353 nucleotides BWI76_RS11190 and BWI76_RS11195 are separated by 249 nucleotides BWI76_RS11195 and BWI76_RS11200 are separated by 218 nucleotides
BWI76_RS11185: BWI76_RS11185 - ligand-gated channel protein, at 2,356,798 to 2,358,975
_RS11185
BWI76_RS11190: BWI76_RS11190 - putative TetR/AcrR family transcriptional regulatory protein, at 2,359,329 to 2,359,964
_RS11190
BWI76_RS11195: BWI76_RS11195 - multiple stress resistance protein BhsA, at 2,360,214 to 2,360,471
_RS11195
BWI76_RS11200: BWI76_RS11200 - transpeptidase, at 2,360,690 to 2,361,649
_RS11200
Position (kb)
2359
2360 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1 at 2358.678 kb on - strand, within BWI76_RS11185 at 2359.082 kb on + strand at 2359.090 kb on - strand at 2359.095 kb on - strand at 2359.256 kb on - strand at 2359.399 kb on + strand, within BWI76_RS11190 at 2359.895 kb on - strand, within BWI76_RS11190 at 2360.202 kb on + strand at 2360.420 kb on + strand, within BWI76_RS11195 at 2360.529 kb on - strand at 2360.737 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction RCH2_defined_noCarbon with Mixed culture; Ralstonia sp. UNC404CL21Col 3:1 starting OD to Klebsiella and Glu_DLac_Acetate_mix 1 x remove 2,358,678 - BWI76_RS11185 0.86 -0.7 2,359,082 + +0.2 2,359,090 - -1.3 2,359,095 - -0.5 2,359,256 - +1.3 2,359,399 + BWI76_RS11190 0.11 -4.5 2,359,895 - BWI76_RS11190 0.89 -4.0 2,360,202 + +0.6 2,360,420 + BWI76_RS11195 0.80 +0.4 2,360,529 - +0.5 2,360,737 + +0.8
Or see this region's nucleotide sequence