Experiment: RCH2_defined_noCarbon with Mixed culture; Ralstonia sp. UNC404CL21Col 3:1 starting OD to Klebsiella and Glu_DLac_Acetate_mix 1 x
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS10975 and BWI76_RS10980 are separated by 124 nucleotides BWI76_RS10980 and BWI76_RS10985 are separated by 285 nucleotides BWI76_RS10985 and BWI76_RS10990 are separated by 72 nucleotides
BWI76_RS10975: BWI76_RS10975 - N-methyltryptophan oxidase, at 2,316,229 to 2,317,347
_RS10975
BWI76_RS10980: BWI76_RS10980 - biofilm formation regulatory protein BssS, at 2,317,472 to 2,317,726
_RS10980
BWI76_RS10985: BWI76_RS10985 - DNA damage-inducible protein I, at 2,318,012 to 2,318,257
_RS10985
BWI76_RS10990: BWI76_RS10990 - dihydroorotase, at 2,318,330 to 2,319,376
_RS10990
Position (kb)
2317
2318 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2
3 at 2316.539 kb on - strand, within BWI76_RS10975 at 2316.610 kb on - strand, within BWI76_RS10975 at 2317.096 kb on + strand, within BWI76_RS10975 at 2317.133 kb on + strand, within BWI76_RS10975 at 2317.198 kb on + strand, within BWI76_RS10975 at 2317.267 kb on + strand at 2317.274 kb on - strand at 2317.284 kb on + strand at 2317.284 kb on + strand at 2317.294 kb on + strand at 2317.337 kb on - strand at 2317.344 kb on + strand at 2317.352 kb on + strand at 2317.361 kb on - strand at 2317.367 kb on - strand at 2317.433 kb on - strand at 2317.492 kb on - strand at 2317.497 kb on - strand at 2317.517 kb on - strand, within BWI76_RS10980 at 2317.518 kb on - strand, within BWI76_RS10980 at 2317.638 kb on + strand, within BWI76_RS10980 at 2317.643 kb on + strand, within BWI76_RS10980 at 2317.670 kb on + strand, within BWI76_RS10980 at 2317.676 kb on - strand, within BWI76_RS10980 at 2317.680 kb on + strand, within BWI76_RS10980 at 2317.690 kb on + strand, within BWI76_RS10980 at 2317.697 kb on + strand, within BWI76_RS10980 at 2317.697 kb on + strand, within BWI76_RS10980 at 2317.698 kb on - strand, within BWI76_RS10980 at 2317.705 kb on - strand at 2317.705 kb on - strand at 2317.726 kb on + strand at 2317.927 kb on + strand at 2317.935 kb on - strand at 2317.945 kb on - strand at 2318.025 kb on + strand at 2318.033 kb on - strand at 2318.033 kb on - strand at 2318.035 kb on - strand at 2318.045 kb on - strand, within BWI76_RS10985 at 2318.134 kb on + strand, within BWI76_RS10985 at 2318.152 kb on - strand, within BWI76_RS10985 at 2318.193 kb on - strand, within BWI76_RS10985 at 2318.193 kb on - strand, within BWI76_RS10985 at 2318.439 kb on + strand, within BWI76_RS10990 at 2318.567 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction RCH2_defined_noCarbon with Mixed culture; Ralstonia sp. UNC404CL21Col 3:1 starting OD to Klebsiella and Glu_DLac_Acetate_mix 1 x remove 2,316,539 - BWI76_RS10975 0.28 +0.2 2,316,610 - BWI76_RS10975 0.34 -3.0 2,317,096 + BWI76_RS10975 0.77 -0.7 2,317,133 + BWI76_RS10975 0.81 -0.5 2,317,198 + BWI76_RS10975 0.87 +0.0 2,317,267 + -0.2 2,317,274 - +1.0 2,317,284 + +0.8 2,317,284 + -0.7 2,317,294 + -0.1 2,317,337 - +0.2 2,317,344 + -1.2 2,317,352 + +0.1 2,317,361 - +0.9 2,317,367 - +1.9 2,317,433 - -0.7 2,317,492 - +0.9 2,317,497 - -0.3 2,317,517 - BWI76_RS10980 0.18 +0.3 2,317,518 - BWI76_RS10980 0.18 -0.3 2,317,638 + BWI76_RS10980 0.65 +2.9 2,317,643 + BWI76_RS10980 0.67 -1.5 2,317,670 + BWI76_RS10980 0.78 -1.4 2,317,676 - BWI76_RS10980 0.80 -3.4 2,317,680 + BWI76_RS10980 0.82 +0.5 2,317,690 + BWI76_RS10980 0.85 -0.2 2,317,697 + BWI76_RS10980 0.88 +0.1 2,317,697 + BWI76_RS10980 0.88 -3.4 2,317,698 - BWI76_RS10980 0.89 -1.2 2,317,705 - -0.2 2,317,705 - -4.5 2,317,726 + +0.2 2,317,927 + -2.3 2,317,935 - +0.6 2,317,945 - -0.1 2,318,025 + -0.3 2,318,033 - -1.6 2,318,033 - -1.1 2,318,035 - +0.7 2,318,045 - BWI76_RS10985 0.13 +0.0 2,318,134 + BWI76_RS10985 0.50 -0.6 2,318,152 - BWI76_RS10985 0.57 +0.4 2,318,193 - BWI76_RS10985 0.74 +0.5 2,318,193 - BWI76_RS10985 0.74 -0.4 2,318,439 + BWI76_RS10990 0.10 -1.7 2,318,567 + +0.9
Or see this region's nucleotide sequence