Experiment: RCH2_defined_noCarbon with Mixed culture; Ralstonia sp. UNC404CL21Col 3:1 starting OD to Klebsiella and Glu_DLac_Acetate_mix 1 x 
Only strains with sufficient reads to estimate fitness are shown,
                   but the strain fitness values are still rather noisy and may be biased towards zero.
                   Strains near the edge of a gene are not shown as being associated with that
                   gene (they are in grey).
                   Strains in the central 10-90% of a gene are color coded by the insertion's strand.
                   Usually, "+" means that the selectable marker is encoded on the forward strand,
                   i.e., transcribed rightward. 
    
        
         
    
        
         
    
        
         
    
        
         
    
         
     
500 nt BWI76_RS10930 and BWI76_RS10935 overlap by 8 nucleotides BWI76_RS10935 and BWI76_RS10940 are separated by 66 nucleotides BWI76_RS10940 and BWI76_RS10945 are separated by 0 nucleotides BWI76_RS10945 and BWI76_RS10950 are separated by 79 nucleotides 
        BWI76_RS10930: BWI76_RS10930 - glucan biosynthesis protein G, at 2,307,601 to 2,309,154 
        _RS10930 
         
        
        BWI76_RS10935: BWI76_RS10935 - glucan biosynthesis glucosyltransferase H, at 2,309,147 to 2,311,675 
        _RS10935 
         
        
        BWI76_RS10940: BWI76_RS10940 - YceK/YidQ family lipoprotein, at 2,311,742 to 2,311,969 
        _RS10940 
         
        
        BWI76_RS10945: BWI76_RS10945 - SecY/SecA suppressor protein, at 2,311,970 to 2,312,356 
        _RS10945 
         
        
        BWI76_RS10950: BWI76_RS10950 - MFS transporter, at 2,312,436 to 2,313,686 
        _RS10950 
         Position (kb) 2309 
2310 
2311 
2312 Strain fitness (log2 ratio) -3 
-2 
-1 
0 
1 at 2308.325 kb on - strand, within BWI76_RS10930 at 2308.504 kb on - strand, within BWI76_RS10930 at 2308.544 kb on + strand, within BWI76_RS10930 at 2308.633 kb on - strand, within BWI76_RS10930 at 2308.882 kb on + strand, within BWI76_RS10930 at 2309.377 kb on + strand at 2309.383 kb on - strand at 2309.655 kb on - strand, within BWI76_RS10935 at 2309.778 kb on - strand, within BWI76_RS10935 at 2309.847 kb on + strand, within BWI76_RS10935 at 2310.070 kb on - strand, within BWI76_RS10935 at 2310.385 kb on + strand, within BWI76_RS10935 at 2310.395 kb on - strand, within BWI76_RS10935 at 2310.924 kb on - strand, within BWI76_RS10935 at 2311.155 kb on - strand, within BWI76_RS10935 at 2312.304 kb on - strand, within BWI76_RS10945 at 2312.539 kb on - strand  
 
 
Per-strain Table 
Position  Strand  Gene  LocusTag  Fraction RCH2_defined_noCarbon with Mixed culture; Ralstonia sp. UNC404CL21Col 3:1 starting OD to Klebsiella and Glu_DLac_Acetate_mix 1 x remove 2,308,325 -  BWI76_RS10930 0.47  -0.1 2,308,504 -  BWI76_RS10930 0.58  -0.7 2,308,544 +  BWI76_RS10930 0.61  -3.3 2,308,633 -  BWI76_RS10930 0.66  -2.5 2,308,882 +  BWI76_RS10930 0.82  -2.6 2,309,377 +  -1.7 2,309,383 -  -2.6 2,309,655 -  BWI76_RS10935 0.20  -1.9 2,309,778 -  BWI76_RS10935 0.25  -3.4 2,309,847 +  BWI76_RS10935 0.28  -1.9 2,310,070 -  BWI76_RS10935 0.36  +0.9 2,310,385 +  BWI76_RS10935 0.49  -1.9 2,310,395 -  BWI76_RS10935 0.49  -0.1 2,310,924 -  BWI76_RS10935 0.70  -1.1 2,311,155 -  BWI76_RS10935 0.79  +0.9 2,312,304 -  BWI76_RS10945 0.86  +0.4 2,312,539 -  +0.2 
Or see this region's nucleotide sequence