Experiment: RCH2_defined_noCarbon with Mixed culture; Ralstonia sp. UNC404CL21Col 3:1 starting OD to Klebsiella and Glu_DLac_Acetate_mix 1 x
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS10650 and BWI76_RS10655 are separated by 15 nucleotides BWI76_RS10655 and BWI76_RS10660 overlap by 35 nucleotides BWI76_RS10660 and BWI76_RS10665 are separated by 51 nucleotides BWI76_RS10665 and BWI76_RS10670 are separated by 20 nucleotides
BWI76_RS10650: BWI76_RS10650 - PTS fructose-like transporter subunit EIIB, at 2,248,865 to 2,249,206
_RS10650
BWI76_RS10655: BWI76_RS10655 - [formate-C-acetyltransferase]-activating enzyme, at 2,249,222 to 2,250,097
_RS10655
BWI76_RS10660: BWI76_RS10660 - formate C-acetyltransferase/glycerol dehydratase family glycyl radical enzyme, at 2,250,063 to 2,252,360
_RS10660
BWI76_RS10665: BWI76_RS10665 - PTS system fructose-like transporter subunit EIIB, at 2,252,412 to 2,252,732
_RS10665
BWI76_RS10670: BWI76_RS10670 - PTS fructose transporter subunit IIC, at 2,252,753 to 2,253,829
_RS10670
Position (kb)
2250
2251
2252
2253 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2
3 at 2249.148 kb on + strand, within BWI76_RS10650 at 2249.150 kb on + strand, within BWI76_RS10650 at 2249.156 kb on - strand, within BWI76_RS10650 at 2249.156 kb on - strand, within BWI76_RS10650 at 2249.389 kb on + strand, within BWI76_RS10655 at 2249.389 kb on + strand, within BWI76_RS10655 at 2249.389 kb on + strand, within BWI76_RS10655 at 2249.397 kb on - strand, within BWI76_RS10655 at 2249.397 kb on - strand, within BWI76_RS10655 at 2250.094 kb on - strand at 2250.097 kb on + strand at 2250.243 kb on - strand at 2250.342 kb on - strand, within BWI76_RS10660 at 2250.378 kb on - strand, within BWI76_RS10660 at 2250.881 kb on - strand, within BWI76_RS10660 at 2250.931 kb on + strand, within BWI76_RS10660 at 2250.993 kb on - strand, within BWI76_RS10660 at 2251.003 kb on - strand, within BWI76_RS10660 at 2251.246 kb on - strand, within BWI76_RS10660 at 2251.384 kb on - strand, within BWI76_RS10660 at 2251.606 kb on - strand, within BWI76_RS10660 at 2251.744 kb on - strand, within BWI76_RS10660 at 2251.746 kb on + strand, within BWI76_RS10660 at 2251.766 kb on - strand, within BWI76_RS10660 at 2251.904 kb on - strand, within BWI76_RS10660 at 2251.904 kb on - strand, within BWI76_RS10660 at 2251.948 kb on + strand, within BWI76_RS10660 at 2252.107 kb on + strand, within BWI76_RS10660 at 2252.677 kb on + strand, within BWI76_RS10665 at 2252.682 kb on + strand, within BWI76_RS10665 at 2252.685 kb on - strand, within BWI76_RS10665 at 2252.790 kb on + strand at 2252.823 kb on + strand at 2252.841 kb on - strand at 2252.934 kb on + strand, within BWI76_RS10670 at 2252.934 kb on + strand, within BWI76_RS10670 at 2252.980 kb on - strand, within BWI76_RS10670 at 2253.276 kb on + strand, within BWI76_RS10670
Per-strain Table
Position Strand Gene LocusTag Fraction RCH2_defined_noCarbon with Mixed culture; Ralstonia sp. UNC404CL21Col 3:1 starting OD to Klebsiella and Glu_DLac_Acetate_mix 1 x remove 2,249,148 + BWI76_RS10650 0.83 +0.3 2,249,150 + BWI76_RS10650 0.83 -1.4 2,249,156 - BWI76_RS10650 0.85 -1.1 2,249,156 - BWI76_RS10650 0.85 +0.4 2,249,389 + BWI76_RS10655 0.19 -0.1 2,249,389 + BWI76_RS10655 0.19 +0.9 2,249,389 + BWI76_RS10655 0.19 +3.5 2,249,397 - BWI76_RS10655 0.20 -3.9 2,249,397 - BWI76_RS10655 0.20 +1.3 2,250,094 - -1.0 2,250,097 + -1.7 2,250,243 - -0.0 2,250,342 - BWI76_RS10660 0.12 +1.0 2,250,378 - BWI76_RS10660 0.14 +0.3 2,250,881 - BWI76_RS10660 0.36 -0.1 2,250,931 + BWI76_RS10660 0.38 -0.7 2,250,993 - BWI76_RS10660 0.40 +1.3 2,251,003 - BWI76_RS10660 0.41 -0.5 2,251,246 - BWI76_RS10660 0.51 -1.4 2,251,384 - BWI76_RS10660 0.57 -3.1 2,251,606 - BWI76_RS10660 0.67 +0.1 2,251,744 - BWI76_RS10660 0.73 +1.3 2,251,746 + BWI76_RS10660 0.73 +0.7 2,251,766 - BWI76_RS10660 0.74 +0.8 2,251,904 - BWI76_RS10660 0.80 -1.4 2,251,904 - BWI76_RS10660 0.80 +0.2 2,251,948 + BWI76_RS10660 0.82 -1.9 2,252,107 + BWI76_RS10660 0.89 +0.9 2,252,677 + BWI76_RS10665 0.83 -0.2 2,252,682 + BWI76_RS10665 0.84 -1.9 2,252,685 - BWI76_RS10665 0.85 +0.9 2,252,790 + +0.3 2,252,823 + -0.7 2,252,841 - -2.4 2,252,934 + BWI76_RS10670 0.17 +0.7 2,252,934 + BWI76_RS10670 0.17 -1.9 2,252,980 - BWI76_RS10670 0.21 +0.4 2,253,276 + BWI76_RS10670 0.49 -0.1
Or see this region's nucleotide sequence