Experiment: RCH2_defined_noCarbon with Mixed culture; Ralstonia sp. UNC404CL21Col 3:1 starting OD to Klebsiella and Glu_DLac_Acetate_mix 1 x
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS10610 and BWI76_RS10615 are separated by 182 nucleotides BWI76_RS10615 and BWI76_RS10620 are separated by 130 nucleotides BWI76_RS10620 and BWI76_RS10625 are separated by 48 nucleotides BWI76_RS10625 and BWI76_RS10630 are separated by 200 nucleotides
BWI76_RS10610: BWI76_RS10610 - helix-turn-helix transcriptional regulator, at 2,241,450 to 2,241,998
_RS10610
BWI76_RS10615: BWI76_RS10615 - chorismate mutase, at 2,242,181 to 2,242,477
_RS10615
BWI76_RS10620: BWI76_RS10620 - hypothetical protein, at 2,242,608 to 2,243,672
_RS10620
BWI76_RS10625: BWI76_RS10625 - hypothetical protein, at 2,243,721 to 2,244,293
_RS10625
BWI76_RS10630: BWI76_RS10630 - LuxR family transcriptional regulator, at 2,244,494 to 2,245,567
_RS10630
Position (kb)
2242
2243
2244 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2
3 at 2241.611 kb on + strand, within BWI76_RS10610 at 2241.616 kb on + strand, within BWI76_RS10610 at 2241.725 kb on + strand, within BWI76_RS10610 at 2241.892 kb on + strand, within BWI76_RS10610 at 2242.116 kb on + strand at 2242.214 kb on - strand, within BWI76_RS10615 at 2242.302 kb on + strand, within BWI76_RS10615 at 2242.308 kb on - strand, within BWI76_RS10615 at 2242.408 kb on + strand, within BWI76_RS10615 at 2242.408 kb on - strand, within BWI76_RS10615 at 2242.422 kb on + strand at 2242.422 kb on + strand, within BWI76_RS10615 at 2242.430 kb on - strand, within BWI76_RS10615 at 2242.452 kb on + strand at 2242.545 kb on + strand at 2242.567 kb on - strand at 2242.662 kb on + strand at 2242.707 kb on + strand at 2242.760 kb on + strand, within BWI76_RS10620 at 2242.796 kb on + strand, within BWI76_RS10620 at 2242.888 kb on + strand, within BWI76_RS10620 at 2242.922 kb on - strand, within BWI76_RS10620 at 2242.938 kb on + strand, within BWI76_RS10620 at 2242.943 kb on + strand, within BWI76_RS10620 at 2242.949 kb on - strand, within BWI76_RS10620 at 2242.951 kb on - strand, within BWI76_RS10620 at 2243.086 kb on + strand at 2243.094 kb on - strand, within BWI76_RS10620 at 2243.133 kb on - strand, within BWI76_RS10620 at 2243.168 kb on - strand, within BWI76_RS10620 at 2243.194 kb on + strand, within BWI76_RS10620 at 2243.217 kb on - strand, within BWI76_RS10620 at 2243.449 kb on + strand, within BWI76_RS10620 at 2243.450 kb on + strand, within BWI76_RS10620 at 2243.464 kb on + strand, within BWI76_RS10620 at 2243.472 kb on + strand, within BWI76_RS10620 at 2243.472 kb on - strand, within BWI76_RS10620 at 2243.482 kb on - strand, within BWI76_RS10620 at 2243.484 kb on + strand, within BWI76_RS10620 at 2243.538 kb on - strand, within BWI76_RS10620 at 2243.595 kb on + strand at 2243.599 kb on + strand at 2243.608 kb on + strand at 2243.608 kb on + strand at 2243.618 kb on - strand at 2243.618 kb on - strand at 2243.629 kb on + strand at 2243.655 kb on - strand at 2243.710 kb on - strand at 2243.726 kb on - strand at 2243.734 kb on + strand at 2243.740 kb on - strand at 2243.742 kb on - strand at 2243.888 kb on - strand, within BWI76_RS10625 at 2243.956 kb on + strand, within BWI76_RS10625 at 2243.956 kb on + strand, within BWI76_RS10625 at 2243.966 kb on + strand, within BWI76_RS10625 at 2243.966 kb on + strand, within BWI76_RS10625 at 2243.966 kb on + strand, within BWI76_RS10625 at 2243.966 kb on + strand, within BWI76_RS10625 at 2243.966 kb on + strand, within BWI76_RS10625 at 2243.966 kb on + strand, within BWI76_RS10625 at 2243.966 kb on + strand at 2243.974 kb on - strand, within BWI76_RS10625 at 2243.974 kb on - strand, within BWI76_RS10625 at 2243.974 kb on - strand, within BWI76_RS10625 at 2243.974 kb on - strand, within BWI76_RS10625 at 2243.974 kb on - strand, within BWI76_RS10625 at 2243.974 kb on - strand, within BWI76_RS10625 at 2243.974 kb on - strand, within BWI76_RS10625 at 2244.023 kb on - strand, within BWI76_RS10625 at 2244.024 kb on - strand, within BWI76_RS10625 at 2244.024 kb on - strand, within BWI76_RS10625 at 2244.031 kb on + strand, within BWI76_RS10625 at 2244.031 kb on + strand, within BWI76_RS10625 at 2244.032 kb on - strand, within BWI76_RS10625 at 2244.039 kb on - strand at 2244.039 kb on - strand, within BWI76_RS10625 at 2244.039 kb on - strand, within BWI76_RS10625 at 2244.039 kb on - strand, within BWI76_RS10625 at 2244.047 kb on - strand, within BWI76_RS10625 at 2244.082 kb on + strand, within BWI76_RS10625 at 2244.087 kb on + strand, within BWI76_RS10625 at 2244.090 kb on - strand, within BWI76_RS10625 at 2244.090 kb on - strand, within BWI76_RS10625 at 2244.090 kb on - strand, within BWI76_RS10625 at 2244.090 kb on - strand, within BWI76_RS10625 at 2244.144 kb on - strand, within BWI76_RS10625 at 2244.188 kb on + strand, within BWI76_RS10625 at 2244.201 kb on - strand, within BWI76_RS10625 at 2244.212 kb on + strand, within BWI76_RS10625 at 2244.348 kb on - strand at 2244.387 kb on + strand at 2244.387 kb on + strand at 2244.387 kb on + strand at 2244.387 kb on + strand at 2244.387 kb on + strand at 2244.387 kb on + strand at 2244.388 kb on + strand at 2244.395 kb on - strand at 2244.395 kb on - strand at 2244.395 kb on - strand at 2244.396 kb on - strand at 2244.400 kb on + strand at 2244.400 kb on + strand at 2244.400 kb on + strand at 2244.400 kb on + strand at 2244.400 kb on + strand at 2244.400 kb on + strand at 2244.400 kb on + strand at 2244.400 kb on + strand at 2244.400 kb on + strand at 2244.400 kb on + strand at 2244.400 kb on + strand at 2244.400 kb on + strand at 2244.400 kb on + strand at 2244.400 kb on + strand at 2244.400 kb on + strand at 2244.400 kb on + strand at 2244.400 kb on + strand at 2244.400 kb on + strand at 2244.400 kb on + strand at 2244.400 kb on + strand at 2244.400 kb on + strand at 2244.400 kb on + strand at 2244.400 kb on + strand at 2244.400 kb on + strand at 2244.400 kb on + strand at 2244.400 kb on + strand at 2244.400 kb on + strand at 2244.400 kb on + strand at 2244.400 kb on + strand at 2244.400 kb on + strand at 2244.400 kb on + strand at 2244.400 kb on + strand at 2244.400 kb on + strand at 2244.400 kb on + strand at 2244.400 kb on + strand at 2244.400 kb on + strand at 2244.408 kb on - strand at 2244.408 kb on - strand at 2244.408 kb on - strand at 2244.408 kb on - strand at 2244.408 kb on - strand at 2244.408 kb on - strand at 2244.408 kb on - strand at 2244.408 kb on - strand at 2244.408 kb on - strand at 2244.408 kb on - strand at 2244.408 kb on - strand at 2244.408 kb on - strand at 2244.408 kb on - strand at 2244.408 kb on - strand at 2244.408 kb on - strand at 2244.408 kb on - strand at 2244.408 kb on - strand at 2244.408 kb on - strand at 2244.408 kb on - strand at 2244.408 kb on - strand at 2244.408 kb on - strand at 2244.408 kb on - strand at 2244.408 kb on - strand at 2244.432 kb on + strand at 2244.432 kb on + strand at 2244.432 kb on + strand at 2244.432 kb on - strand at 2244.432 kb on - strand at 2244.437 kb on + strand at 2244.437 kb on + strand at 2244.437 kb on + strand at 2244.437 kb on + strand at 2244.437 kb on + strand at 2244.437 kb on + strand at 2244.437 kb on + strand at 2244.437 kb on + strand at 2244.440 kb on - strand at 2244.442 kb on + strand at 2244.445 kb on - strand at 2244.445 kb on - strand at 2244.445 kb on - strand at 2244.445 kb on - strand at 2244.445 kb on - strand at 2244.445 kb on - strand at 2244.445 kb on - strand at 2244.445 kb on - strand at 2244.445 kb on - strand at 2244.445 kb on - strand at 2244.445 kb on - strand at 2244.445 kb on - strand at 2244.445 kb on - strand at 2244.445 kb on - strand at 2244.445 kb on - strand at 2244.445 kb on - strand at 2244.445 kb on - strand at 2244.445 kb on - strand at 2244.445 kb on - strand at 2244.445 kb on - strand at 2244.445 kb on - strand at 2244.445 kb on - strand at 2244.450 kb on - strand at 2244.450 kb on - strand at 2244.455 kb on + strand at 2244.502 kb on + strand at 2244.526 kb on + strand at 2244.535 kb on + strand at 2244.535 kb on + strand at 2244.541 kb on + strand at 2244.541 kb on + strand at 2244.542 kb on + strand at 2244.542 kb on + strand at 2244.552 kb on + strand at 2244.558 kb on - strand at 2244.564 kb on + strand at 2244.564 kb on + strand at 2244.566 kb on + strand at 2244.576 kb on + strand at 2244.600 kb on + strand at 2244.600 kb on + strand at 2244.610 kb on + strand, within BWI76_RS10630 at 2244.618 kb on - strand, within BWI76_RS10630 at 2244.619 kb on + strand, within BWI76_RS10630 at 2244.633 kb on + strand, within BWI76_RS10630 at 2244.633 kb on + strand, within BWI76_RS10630 at 2244.641 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction RCH2_defined_noCarbon with Mixed culture; Ralstonia sp. UNC404CL21Col 3:1 starting OD to Klebsiella and Glu_DLac_Acetate_mix 1 x remove 2,241,611 + BWI76_RS10610 0.29 +1.1 2,241,616 + BWI76_RS10610 0.30 -0.7 2,241,725 + BWI76_RS10610 0.50 +1.1 2,241,892 + BWI76_RS10610 0.81 +0.9 2,242,116 + -1.9 2,242,214 - BWI76_RS10615 0.11 +1.6 2,242,302 + BWI76_RS10615 0.41 -2.9 2,242,308 - BWI76_RS10615 0.43 +0.1 2,242,408 + BWI76_RS10615 0.76 -3.6 2,242,408 - BWI76_RS10615 0.76 +0.5 2,242,422 + -1.7 2,242,422 + BWI76_RS10615 0.81 +0.0 2,242,430 - BWI76_RS10615 0.84 +0.3 2,242,452 + -0.1 2,242,545 + +1.2 2,242,567 - +0.2 2,242,662 + +0.9 2,242,707 + -0.7 2,242,760 + BWI76_RS10620 0.14 -1.9 2,242,796 + BWI76_RS10620 0.18 +0.3 2,242,888 + BWI76_RS10620 0.26 +0.6 2,242,922 - BWI76_RS10620 0.29 +0.1 2,242,938 + BWI76_RS10620 0.31 -3.7 2,242,943 + BWI76_RS10620 0.31 -1.1 2,242,949 - BWI76_RS10620 0.32 -0.7 2,242,951 - BWI76_RS10620 0.32 -2.3 2,243,086 + -0.1 2,243,094 - BWI76_RS10620 0.46 +0.0 2,243,133 - BWI76_RS10620 0.49 -1.7 2,243,168 - BWI76_RS10620 0.53 +0.9 2,243,194 + BWI76_RS10620 0.55 -0.1 2,243,217 - BWI76_RS10620 0.57 -1.9 2,243,449 + BWI76_RS10620 0.79 -2.1 2,243,450 + BWI76_RS10620 0.79 -2.1 2,243,464 + BWI76_RS10620 0.80 -0.2 2,243,472 + BWI76_RS10620 0.81 +0.3 2,243,472 - BWI76_RS10620 0.81 +0.3 2,243,482 - BWI76_RS10620 0.82 -0.1 2,243,484 + BWI76_RS10620 0.82 -0.1 2,243,538 - BWI76_RS10620 0.87 -1.3 2,243,595 + -1.1 2,243,599 + -1.1 2,243,608 + -0.8 2,243,608 + +0.9 2,243,618 - -0.1 2,243,618 - +1.0 2,243,629 + -2.3 2,243,655 - -2.9 2,243,710 - +0.0 2,243,726 - +0.0 2,243,734 + +0.9 2,243,740 - -3.3 2,243,742 - +0.4 2,243,888 - BWI76_RS10625 0.29 -2.3 2,243,956 + BWI76_RS10625 0.41 -0.4 2,243,956 + BWI76_RS10625 0.41 -2.7 2,243,966 + BWI76_RS10625 0.43 -2.5 2,243,966 + BWI76_RS10625 0.43 -1.7 2,243,966 + BWI76_RS10625 0.43 -1.9 2,243,966 + BWI76_RS10625 0.43 -1.4 2,243,966 + BWI76_RS10625 0.43 -2.1 2,243,966 + BWI76_RS10625 0.43 -1.7 2,243,966 + +0.9 2,243,974 - BWI76_RS10625 0.44 -0.7 2,243,974 - BWI76_RS10625 0.44 -0.5 2,243,974 - BWI76_RS10625 0.44 -0.7 2,243,974 - BWI76_RS10625 0.44 -1.1 2,243,974 - BWI76_RS10625 0.44 +0.4 2,243,974 - BWI76_RS10625 0.44 +3.6 2,243,974 - BWI76_RS10625 0.44 -2.5 2,244,023 - BWI76_RS10625 0.53 -1.8 2,244,024 - BWI76_RS10625 0.53 -2.0 2,244,024 - BWI76_RS10625 0.53 +0.8 2,244,031 + BWI76_RS10625 0.54 -1.7 2,244,031 + BWI76_RS10625 0.54 -0.4 2,244,032 - BWI76_RS10625 0.54 -0.7 2,244,039 - -0.1 2,244,039 - BWI76_RS10625 0.55 -1.7 2,244,039 - BWI76_RS10625 0.55 -1.2 2,244,039 - BWI76_RS10625 0.55 -1.4 2,244,047 - BWI76_RS10625 0.57 -1.3 2,244,082 + BWI76_RS10625 0.63 +0.9 2,244,087 + BWI76_RS10625 0.64 -0.1 2,244,090 - BWI76_RS10625 0.64 -1.1 2,244,090 - BWI76_RS10625 0.64 -1.9 2,244,090 - BWI76_RS10625 0.64 -2.3 2,244,090 - BWI76_RS10625 0.64 +1.7 2,244,144 - BWI76_RS10625 0.74 +2.9 2,244,188 + BWI76_RS10625 0.82 -2.3 2,244,201 - BWI76_RS10625 0.84 -1.9 2,244,212 + BWI76_RS10625 0.86 -1.1 2,244,348 - -0.7 2,244,387 + +1.6 2,244,387 + +0.9 2,244,387 + -2.1 2,244,387 + +3.7 2,244,387 + -1.3 2,244,387 + -1.1 2,244,388 + -2.1 2,244,395 - -1.4 2,244,395 - -1.1 2,244,395 - -1.1 2,244,396 - -3.9 2,244,400 + -1.1 2,244,400 + +1.1 2,244,400 + -2.9 2,244,400 + -0.7 2,244,400 + -2.7 2,244,400 + -1.1 2,244,400 + -0.1 2,244,400 + -2.9 2,244,400 + -1.1 2,244,400 + -1.1 2,244,400 + +2.9 2,244,400 + -1.1 2,244,400 + -1.0 2,244,400 + -0.7 2,244,400 + +0.8 2,244,400 + +2.1 2,244,400 + +1.2 2,244,400 + -0.7 2,244,400 + -0.1 2,244,400 + -3.2 2,244,400 + -2.8 2,244,400 + -1.9 2,244,400 + -3.0 2,244,400 + -1.1 2,244,400 + +0.3 2,244,400 + -3.3 2,244,400 + -0.7 2,244,400 + +0.9 2,244,400 + -0.7 2,244,400 + -0.1 2,244,400 + -0.5 2,244,400 + -3.5 2,244,400 + +1.0 2,244,400 + -2.8 2,244,400 + -0.1 2,244,400 + -1.1 2,244,408 - -1.7 2,244,408 - +1.3 2,244,408 - +0.9 2,244,408 - -0.7 2,244,408 - -1.9 2,244,408 - -0.1 2,244,408 - -1.4 2,244,408 - -0.7 2,244,408 - -0.1 2,244,408 - -0.1 2,244,408 - -2.5 2,244,408 - -2.1 2,244,408 - -2.2 2,244,408 - +1.5 2,244,408 - -0.1 2,244,408 - +0.9 2,244,408 - +0.9 2,244,408 - -2.1 2,244,408 - +0.9 2,244,408 - +0.2 2,244,408 - -0.8 2,244,408 - -0.3 2,244,408 - -0.7 2,244,432 + -0.1 2,244,432 + -2.7 2,244,432 + -0.1 2,244,432 - -0.1 2,244,432 - +1.2 2,244,437 + +0.4 2,244,437 + -1.4 2,244,437 + -0.8 2,244,437 + -2.8 2,244,437 + -0.1 2,244,437 + +1.4 2,244,437 + +0.9 2,244,437 + -2.4 2,244,440 - +0.4 2,244,442 + -1.4 2,244,445 - +0.5 2,244,445 - +0.4 2,244,445 - +0.6 2,244,445 - -0.1 2,244,445 - -0.5 2,244,445 - +0.7 2,244,445 - -1.7 2,244,445 - +0.6 2,244,445 - +0.9 2,244,445 - +0.7 2,244,445 - +0.9 2,244,445 - -0.5 2,244,445 - -0.9 2,244,445 - +1.1 2,244,445 - -2.3 2,244,445 - -3.5 2,244,445 - +0.8 2,244,445 - -0.1 2,244,445 - +0.4 2,244,445 - -0.7 2,244,445 - -1.4 2,244,445 - -0.1 2,244,450 - +0.9 2,244,450 - -1.9 2,244,455 + -1.0 2,244,502 + +1.6 2,244,526 + -2.7 2,244,535 + +2.0 2,244,535 + -0.8 2,244,541 + -1.4 2,244,541 + +0.9 2,244,542 + -1.8 2,244,542 + -0.8 2,244,552 + -3.2 2,244,558 - +0.9 2,244,564 + -2.1 2,244,564 + +1.7 2,244,566 + -1.1 2,244,576 + +0.4 2,244,600 + -0.5 2,244,600 + -1.1 2,244,610 + BWI76_RS10630 0.11 -2.1 2,244,618 - BWI76_RS10630 0.12 -1.1 2,244,619 + BWI76_RS10630 0.12 -0.5 2,244,633 + BWI76_RS10630 0.13 -3.0 2,244,633 + BWI76_RS10630 0.13 +0.2 2,244,641 - +0.9
Or see this region's nucleotide sequence