Experiment: RCH2_defined_noCarbon with Mixed culture; Ralstonia sp. UNC404CL21Col 3:1 starting OD to Klebsiella and Glu_DLac_Acetate_mix 1 x
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS09995 and BWI76_RS10000 are separated by 28 nucleotides BWI76_RS10000 and BWI76_RS10005 are separated by 154 nucleotides
BWI76_RS09995: BWI76_RS09995 - DNA helicase IV, at 2,103,951 to 2,106,005
_RS09995
BWI76_RS10000: BWI76_RS10000 - methylglyoxal synthase, at 2,106,034 to 2,106,492
_RS10000
BWI76_RS10005: BWI76_RS10005 - CoA-binding protein, at 2,106,647 to 2,107,060
_RS10005
Position (kb)
2106
2107 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2 at 2105.437 kb on - strand, within BWI76_RS09995 at 2105.444 kb on + strand, within BWI76_RS09995 at 2105.444 kb on + strand, within BWI76_RS09995 at 2105.534 kb on - strand, within BWI76_RS09995 at 2105.879 kb on - strand at 2105.910 kb on + strand at 2105.910 kb on + strand at 2105.918 kb on - strand at 2105.918 kb on - strand at 2106.056 kb on - strand at 2106.128 kb on + strand, within BWI76_RS10000 at 2106.128 kb on + strand, within BWI76_RS10000 at 2106.197 kb on - strand, within BWI76_RS10000 at 2106.197 kb on - strand, within BWI76_RS10000 at 2106.269 kb on + strand, within BWI76_RS10000 at 2106.277 kb on - strand, within BWI76_RS10000 at 2106.323 kb on - strand, within BWI76_RS10000 at 2106.323 kb on - strand, within BWI76_RS10000 at 2106.323 kb on - strand, within BWI76_RS10000 at 2106.447 kb on + strand at 2106.455 kb on - strand at 2106.524 kb on - strand at 2106.582 kb on + strand at 2106.584 kb on - strand at 2106.626 kb on - strand at 2106.708 kb on - strand, within BWI76_RS10005 at 2106.708 kb on - strand, within BWI76_RS10005 at 2106.720 kb on - strand, within BWI76_RS10005 at 2106.772 kb on + strand, within BWI76_RS10005 at 2106.801 kb on + strand, within BWI76_RS10005 at 2106.804 kb on + strand, within BWI76_RS10005 at 2106.817 kb on - strand, within BWI76_RS10005 at 2106.882 kb on + strand, within BWI76_RS10005 at 2106.905 kb on + strand, within BWI76_RS10005 at 2106.949 kb on + strand, within BWI76_RS10005 at 2106.985 kb on - strand, within BWI76_RS10005 at 2106.998 kb on - strand, within BWI76_RS10005 at 2107.007 kb on + strand, within BWI76_RS10005 at 2107.042 kb on + strand at 2107.046 kb on + strand at 2107.061 kb on + strand at 2107.061 kb on + strand at 2107.090 kb on - strand at 2107.091 kb on - strand at 2107.091 kb on - strand at 2107.188 kb on - strand at 2107.196 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction RCH2_defined_noCarbon with Mixed culture; Ralstonia sp. UNC404CL21Col 3:1 starting OD to Klebsiella and Glu_DLac_Acetate_mix 1 x remove 2,105,437 - BWI76_RS09995 0.72 -0.5 2,105,444 + BWI76_RS09995 0.73 +0.6 2,105,444 + BWI76_RS09995 0.73 +0.5 2,105,534 - BWI76_RS09995 0.77 -2.2 2,105,879 - -0.1 2,105,910 + +0.9 2,105,910 + +0.6 2,105,918 - -1.0 2,105,918 - -2.7 2,106,056 - -0.6 2,106,128 + BWI76_RS10000 0.20 -0.2 2,106,128 + BWI76_RS10000 0.20 -1.4 2,106,197 - BWI76_RS10000 0.36 -1.0 2,106,197 - BWI76_RS10000 0.36 +0.1 2,106,269 + BWI76_RS10000 0.51 -1.6 2,106,277 - BWI76_RS10000 0.53 +0.3 2,106,323 - BWI76_RS10000 0.63 +2.1 2,106,323 - BWI76_RS10000 0.63 -3.1 2,106,323 - BWI76_RS10000 0.63 -0.1 2,106,447 + +0.9 2,106,455 - -0.0 2,106,524 - +0.2 2,106,582 + -3.4 2,106,584 - +0.2 2,106,626 - -3.1 2,106,708 - BWI76_RS10005 0.15 +0.7 2,106,708 - BWI76_RS10005 0.15 -3.6 2,106,720 - BWI76_RS10005 0.18 -4.5 2,106,772 + BWI76_RS10005 0.30 -2.9 2,106,801 + BWI76_RS10005 0.37 +0.5 2,106,804 + BWI76_RS10005 0.38 -0.3 2,106,817 - BWI76_RS10005 0.41 -0.7 2,106,882 + BWI76_RS10005 0.57 -0.2 2,106,905 + BWI76_RS10005 0.62 +0.8 2,106,949 + BWI76_RS10005 0.73 -0.2 2,106,985 - BWI76_RS10005 0.82 -0.1 2,106,998 - BWI76_RS10005 0.85 +0.9 2,107,007 + BWI76_RS10005 0.87 -0.6 2,107,042 + +1.8 2,107,046 + +1.5 2,107,061 + +0.7 2,107,061 + -1.0 2,107,090 - -0.7 2,107,091 - +2.2 2,107,091 - -0.4 2,107,188 - -0.1 2,107,196 - -2.3
Or see this region's nucleotide sequence