Experiment: RCH2_defined_noCarbon with Mixed culture; Ralstonia sp. UNC404CL21Col 3:1 starting OD to Klebsiella and Glu_DLac_Acetate_mix 1 x
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS07720 and BWI76_RS07725 overlap by 4 nucleotides BWI76_RS07725 and BWI76_RS07730 overlap by 4 nucleotides BWI76_RS07730 and BWI76_RS07735 are separated by 112 nucleotides
BWI76_RS07720: BWI76_RS07720 - iron-enterobactin transporter ATP-binding protein, at 1,637,776 to 1,638,570
_RS07720
BWI76_RS07725: BWI76_RS07725 - iron-enterobactin transporter permease, at 1,638,567 to 1,639,559
_RS07725
BWI76_RS07730: BWI76_RS07730 - iron-enterobactin transporter membrane protein, at 1,639,556 to 1,640,563
_RS07730
BWI76_RS07735: BWI76_RS07735 - MFS transporter, at 1,640,676 to 1,641,917
_RS07735
Position (kb) 1639
1640
1641 Strain fitness (log2 ratio) -2
-1
0
1 at 1638.661 kb on + strand at 1639.293 kb on + strand, within BWI76_RS07725 at 1640.043 kb on + strand, within BWI76_RS07730 at 1640.527 kb on + strand at 1640.628 kb on + strand at 1640.681 kb on - strand at 1641.025 kb on + strand, within BWI76_RS07735 at 1641.368 kb on + strand, within BWI76_RS07735 at 1641.376 kb on - strand, within BWI76_RS07735 at 1641.389 kb on + strand, within BWI76_RS07735 at 1641.498 kb on + strand, within BWI76_RS07735
Per-strain Table
Position Strand Gene LocusTag Fraction RCH2_defined_noCarbon with Mixed culture; Ralstonia sp. UNC404CL21Col 3:1 starting OD to Klebsiella and Glu_DLac_Acetate_mix 1 x remove 1,638,661 + -2.5 1,639,293 + BWI76_RS07725 0.73 +0.8 1,640,043 + BWI76_RS07730 0.48 -2.1 1,640,527 + -1.5 1,640,628 + -1.7 1,640,681 - +0.2 1,641,025 + BWI76_RS07735 0.28 +0.9 1,641,368 + BWI76_RS07735 0.56 +0.9 1,641,376 - BWI76_RS07735 0.56 +0.9 1,641,389 + BWI76_RS07735 0.57 +0.7 1,641,498 + BWI76_RS07735 0.66 +0.7
Or see this region's nucleotide sequence