Experiment: RCH2_defined_noCarbon with Mixed culture; Ralstonia sp. UNC404CL21Col 3:1 starting OD to Klebsiella and Glu_DLac_Acetate_mix 1 x
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS07560 and BWI76_RS07565 are separated by 308 nucleotides BWI76_RS07565 and BWI76_RS07570 are separated by 24 nucleotides BWI76_RS07570 and BWI76_RS07575 are separated by 234 nucleotides
BWI76_RS07560: BWI76_RS07560 - aminotransferase regulator domain-containing protein, at 1,600,675 to 1,602,009
_RS07560
BWI76_RS07565: BWI76_RS07565 - LysE type translocator family protein, at 1,602,318 to 1,602,959
_RS07565
BWI76_RS07570: BWI76_RS07570 - GntR family transcriptional regulator with, at 1,602,984 to 1,604,450
_RS07570
BWI76_RS07575: BWI76_RS07575 - ABC transporter substrate-binding protein, at 1,604,685 to 1,605,536
_RS07575
Position (kb)
1602
1603
1604
1605 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1 at 1602.055 kb on + strand at 1602.233 kb on + strand at 1602.290 kb on + strand at 1602.290 kb on + strand at 1602.298 kb on - strand at 1602.298 kb on - strand at 1602.308 kb on - strand at 1602.334 kb on + strand at 1602.395 kb on + strand, within BWI76_RS07565 at 1602.419 kb on + strand, within BWI76_RS07565 at 1602.445 kb on - strand, within BWI76_RS07565 at 1602.457 kb on + strand, within BWI76_RS07565 at 1602.463 kb on + strand, within BWI76_RS07565 at 1602.773 kb on + strand, within BWI76_RS07565 at 1602.773 kb on + strand, within BWI76_RS07565 at 1602.781 kb on - strand, within BWI76_RS07565 at 1602.802 kb on - strand, within BWI76_RS07565 at 1602.821 kb on - strand, within BWI76_RS07565 at 1602.835 kb on + strand, within BWI76_RS07565 at 1603.016 kb on + strand at 1603.158 kb on - strand, within BWI76_RS07570 at 1603.162 kb on + strand, within BWI76_RS07570 at 1603.275 kb on - strand, within BWI76_RS07570 at 1603.376 kb on + strand, within BWI76_RS07570 at 1603.400 kb on - strand, within BWI76_RS07570 at 1603.631 kb on - strand, within BWI76_RS07570 at 1604.095 kb on - strand, within BWI76_RS07570 at 1604.144 kb on - strand, within BWI76_RS07570 at 1604.216 kb on + strand, within BWI76_RS07570 at 1604.224 kb on - strand, within BWI76_RS07570 at 1604.238 kb on + strand, within BWI76_RS07570 at 1604.290 kb on - strand, within BWI76_RS07570 at 1604.339 kb on - strand at 1604.439 kb on - strand at 1604.476 kb on + strand at 1604.476 kb on + strand at 1604.484 kb on - strand at 1604.517 kb on - strand at 1604.539 kb on - strand at 1604.598 kb on + strand at 1604.598 kb on + strand at 1604.708 kb on - strand at 1604.719 kb on + strand at 1604.727 kb on - strand at 1604.727 kb on - strand at 1604.789 kb on + strand, within BWI76_RS07575 at 1604.820 kb on + strand, within BWI76_RS07575 at 1604.820 kb on + strand, within BWI76_RS07575 at 1604.828 kb on - strand, within BWI76_RS07575 at 1604.828 kb on - strand, within BWI76_RS07575 at 1604.865 kb on + strand, within BWI76_RS07575 at 1604.914 kb on + strand, within BWI76_RS07575 at 1604.915 kb on - strand, within BWI76_RS07575 at 1604.920 kb on - strand, within BWI76_RS07575 at 1604.922 kb on - strand, within BWI76_RS07575 at 1604.940 kb on - strand, within BWI76_RS07575 at 1604.955 kb on + strand, within BWI76_RS07575 at 1604.964 kb on + strand, within BWI76_RS07575 at 1604.970 kb on + strand, within BWI76_RS07575 at 1604.970 kb on + strand, within BWI76_RS07575 at 1604.970 kb on + strand, within BWI76_RS07575 at 1604.970 kb on + strand, within BWI76_RS07575 at 1604.970 kb on + strand, within BWI76_RS07575 at 1604.978 kb on - strand, within BWI76_RS07575 at 1604.978 kb on - strand, within BWI76_RS07575 at 1604.978 kb on - strand, within BWI76_RS07575 at 1604.978 kb on - strand, within BWI76_RS07575 at 1604.978 kb on - strand, within BWI76_RS07575 at 1604.978 kb on - strand, within BWI76_RS07575 at 1604.978 kb on - strand, within BWI76_RS07575 at 1604.999 kb on - strand, within BWI76_RS07575 at 1605.001 kb on + strand, within BWI76_RS07575 at 1605.002 kb on + strand, within BWI76_RS07575 at 1605.009 kb on - strand, within BWI76_RS07575 at 1605.066 kb on + strand, within BWI76_RS07575 at 1605.074 kb on - strand, within BWI76_RS07575 at 1605.088 kb on + strand, within BWI76_RS07575 at 1605.088 kb on + strand, within BWI76_RS07575 at 1605.091 kb on - strand, within BWI76_RS07575 at 1605.217 kb on + strand, within BWI76_RS07575 at 1605.225 kb on - strand, within BWI76_RS07575 at 1605.227 kb on - strand, within BWI76_RS07575 at 1605.229 kb on + strand, within BWI76_RS07575 at 1605.293 kb on + strand, within BWI76_RS07575 at 1605.299 kb on + strand, within BWI76_RS07575 at 1605.312 kb on - strand, within BWI76_RS07575 at 1605.332 kb on - strand, within BWI76_RS07575 at 1605.391 kb on - strand, within BWI76_RS07575
Per-strain Table
Position Strand Gene LocusTag Fraction RCH2_defined_noCarbon with Mixed culture; Ralstonia sp. UNC404CL21Col 3:1 starting OD to Klebsiella and Glu_DLac_Acetate_mix 1 x remove 1,602,055 + +1.3 1,602,233 + +1.1 1,602,290 + -1.6 1,602,290 + +0.4 1,602,298 - +0.3 1,602,298 - -2.3 1,602,308 - -2.0 1,602,334 + -0.9 1,602,395 + BWI76_RS07565 0.12 -1.2 1,602,419 + BWI76_RS07565 0.16 -0.0 1,602,445 - BWI76_RS07565 0.20 -0.7 1,602,457 + BWI76_RS07565 0.22 -0.1 1,602,463 + BWI76_RS07565 0.23 -0.7 1,602,773 + BWI76_RS07565 0.71 +0.0 1,602,773 + BWI76_RS07565 0.71 -0.4 1,602,781 - BWI76_RS07565 0.72 +0.5 1,602,802 - BWI76_RS07565 0.75 -0.9 1,602,821 - BWI76_RS07565 0.78 -0.1 1,602,835 + BWI76_RS07565 0.81 +0.5 1,603,016 + -0.0 1,603,158 - BWI76_RS07570 0.12 -2.5 1,603,162 + BWI76_RS07570 0.12 -0.1 1,603,275 - BWI76_RS07570 0.20 +0.6 1,603,376 + BWI76_RS07570 0.27 +0.5 1,603,400 - BWI76_RS07570 0.28 -1.0 1,603,631 - BWI76_RS07570 0.44 +0.4 1,604,095 - BWI76_RS07570 0.76 -0.1 1,604,144 - BWI76_RS07570 0.79 +0.4 1,604,216 + BWI76_RS07570 0.84 +0.9 1,604,224 - BWI76_RS07570 0.85 +0.4 1,604,238 + BWI76_RS07570 0.85 +0.8 1,604,290 - BWI76_RS07570 0.89 -3.6 1,604,339 - -0.7 1,604,439 - +0.8 1,604,476 + -0.0 1,604,476 + +0.9 1,604,484 - -0.9 1,604,517 - +0.3 1,604,539 - +0.7 1,604,598 + +0.9 1,604,598 + +0.6 1,604,708 - +0.6 1,604,719 + -0.0 1,604,727 - +0.4 1,604,727 - +1.5 1,604,789 + BWI76_RS07575 0.12 -0.8 1,604,820 + BWI76_RS07575 0.16 +0.0 1,604,820 + BWI76_RS07575 0.16 +1.1 1,604,828 - BWI76_RS07575 0.17 +0.0 1,604,828 - BWI76_RS07575 0.17 -0.7 1,604,865 + BWI76_RS07575 0.21 +0.2 1,604,914 + BWI76_RS07575 0.27 +0.4 1,604,915 - BWI76_RS07575 0.27 +1.5 1,604,920 - BWI76_RS07575 0.28 -1.7 1,604,922 - BWI76_RS07575 0.28 +1.3 1,604,940 - BWI76_RS07575 0.30 -0.9 1,604,955 + BWI76_RS07575 0.32 -1.5 1,604,964 + BWI76_RS07575 0.33 +0.9 1,604,970 + BWI76_RS07575 0.33 -2.5 1,604,970 + BWI76_RS07575 0.33 +1.5 1,604,970 + BWI76_RS07575 0.33 +0.2 1,604,970 + BWI76_RS07575 0.33 +0.5 1,604,970 + BWI76_RS07575 0.33 +1.7 1,604,978 - BWI76_RS07575 0.34 -0.5 1,604,978 - BWI76_RS07575 0.34 -2.6 1,604,978 - BWI76_RS07575 0.34 -0.1 1,604,978 - BWI76_RS07575 0.34 -0.1 1,604,978 - BWI76_RS07575 0.34 -0.6 1,604,978 - BWI76_RS07575 0.34 -0.3 1,604,978 - BWI76_RS07575 0.34 +1.0 1,604,999 - BWI76_RS07575 0.37 -1.5 1,605,001 + BWI76_RS07575 0.37 -3.2 1,605,002 + BWI76_RS07575 0.37 -3.9 1,605,009 - BWI76_RS07575 0.38 +0.4 1,605,066 + BWI76_RS07575 0.45 +0.3 1,605,074 - BWI76_RS07575 0.46 +0.0 1,605,088 + BWI76_RS07575 0.47 -0.5 1,605,088 + BWI76_RS07575 0.47 -0.1 1,605,091 - BWI76_RS07575 0.48 +0.6 1,605,217 + BWI76_RS07575 0.62 -0.3 1,605,225 - BWI76_RS07575 0.63 -0.1 1,605,227 - BWI76_RS07575 0.64 -0.3 1,605,229 + BWI76_RS07575 0.64 +0.9 1,605,293 + BWI76_RS07575 0.71 +0.4 1,605,299 + BWI76_RS07575 0.72 +0.1 1,605,312 - BWI76_RS07575 0.74 +0.7 1,605,332 - BWI76_RS07575 0.76 -0.0 1,605,391 - BWI76_RS07575 0.83 -0.3
Or see this region's nucleotide sequence