Strain Fitness in Klebsiella michiganensis M5al around BWI76_RS05280

Experiment: RCH2_defined_noCarbon with Mixed culture; Ralstonia sp. UNC404CL21Col 3:1 starting OD to Klebsiella and Glu_DLac_Acetate_mix 1 x

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntBWI76_RS05270 and BWI76_RS05275 are separated by 3 nucleotidesBWI76_RS05275 and BWI76_RS05280 overlap by 1 nucleotidesBWI76_RS05280 and BWI76_RS05285 overlap by 4 nucleotidesBWI76_RS05285 and BWI76_RS05290 are separated by 38 nucleotides BWI76_RS05270: BWI76_RS05270 - beta-hydroxyacyl-ACP dehydratase, at 1,109,902 to 1,110,357 _RS05270 BWI76_RS05275: BWI76_RS05275 - acyl-[acyl-carrier-protein]--UDP-N- acetylglucosamine O-acyltransferase, at 1,110,361 to 1,111,149 _RS05275 BWI76_RS05280: BWI76_RS05280 - lipid-A-disaccharide synthase, at 1,111,149 to 1,112,297 _RS05280 BWI76_RS05285: BWI76_RS05285 - ribonuclease HII, at 1,112,294 to 1,112,890 _RS05285 BWI76_RS05290: BWI76_RS05290 - DNA polymerase III subunit alpha, at 1,112,929 to 1,116,411 _RS05290 Position (kb) 1111 1112 1113Strain fitness (log2 ratio) -3 -2 -1 0 1at 1112.362 kb on + strand, within BWI76_RS05285at 1112.850 kb on + strand

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Per-strain Table

Position Strand Gene LocusTag Fraction RCH2_defined_noCarbon with Mixed culture; Ralstonia sp. UNC404CL21Col 3:1 starting OD to Klebsiella and Glu_DLac_Acetate_mix 1 x
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1,112,362 + BWI76_RS05285 0.11 -1.6
1,112,850 + -2.9

Or see this region's nucleotide sequence