Experiment: RCH2_defined_noCarbon with Mixed culture; Ralstonia sp. UNC404CL21Col 3:1 starting OD to Klebsiella and Glu_DLac_Acetate_mix 1 x
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS04895 and BWI76_RS04900 are separated by 50 nucleotides BWI76_RS04900 and BWI76_RS04905 are separated by 10 nucleotides BWI76_RS04905 and BWI76_RS04910 are separated by 334 nucleotides
BWI76_RS04895: BWI76_RS04895 - hypothetical protein, at 1,026,368 to 1,026,652
_RS04895
BWI76_RS04900: BWI76_RS04900 - DUF2950 domain-containing protein, at 1,026,703 to 1,027,497
_RS04900
BWI76_RS04905: BWI76_RS04905 - DUF3300 domain-containing protein, at 1,027,508 to 1,029,094
_RS04905
BWI76_RS04910: BWI76_RS04910 - bifunctional aconitate hydratase 2/2-methylisocitrate dehydratase, at 1,029,429 to 1,032,047
_RS04910
Position (kb)
1027
1028
1029
1030 Strain fitness (log2 ratio)
-3
-2
-1
0
1 at 1026.944 kb on - strand, within BWI76_RS04900 at 1027.291 kb on - strand, within BWI76_RS04900 at 1027.562 kb on - strand at 1027.630 kb on - strand at 1027.667 kb on - strand, within BWI76_RS04905 at 1027.786 kb on + strand, within BWI76_RS04905 at 1028.048 kb on - strand, within BWI76_RS04905 at 1028.268 kb on + strand, within BWI76_RS04905 at 1028.712 kb on - strand, within BWI76_RS04905 at 1028.831 kb on - strand, within BWI76_RS04905 at 1028.846 kb on + strand, within BWI76_RS04905 at 1028.926 kb on - strand, within BWI76_RS04905 at 1028.926 kb on - strand, within BWI76_RS04905 at 1028.926 kb on - strand, within BWI76_RS04905 at 1028.962 kb on - strand at 1029.131 kb on + strand at 1029.241 kb on + strand at 1029.249 kb on - strand at 1029.276 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction RCH2_defined_noCarbon with Mixed culture; Ralstonia sp. UNC404CL21Col 3:1 starting OD to Klebsiella and Glu_DLac_Acetate_mix 1 x remove 1,026,944 - BWI76_RS04900 0.30 -0.4 1,027,291 - BWI76_RS04900 0.74 -3.0 1,027,562 - -1.3 1,027,630 - +1.0 1,027,667 - BWI76_RS04905 0.10 +0.4 1,027,786 + BWI76_RS04905 0.18 +0.5 1,028,048 - BWI76_RS04905 0.34 -0.4 1,028,268 + BWI76_RS04905 0.48 -1.0 1,028,712 - BWI76_RS04905 0.76 +0.7 1,028,831 - BWI76_RS04905 0.83 +1.3 1,028,846 + BWI76_RS04905 0.84 -2.2 1,028,926 - BWI76_RS04905 0.89 +0.0 1,028,926 - BWI76_RS04905 0.89 -0.5 1,028,926 - BWI76_RS04905 0.89 -1.9 1,028,962 - +1.7 1,029,131 + -1.0 1,029,241 + -3.2 1,029,249 - -0.9 1,029,276 - +0.8
Or see this region's nucleotide sequence