Experiment: RCH2_defined_noCarbon with Mixed culture; Ralstonia sp. UNC404CL21Col 3:1 starting OD to Klebsiella and Glu_DLac_Acetate_mix 1 x
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS04650 and BWI76_RS04655 are separated by 11 nucleotides BWI76_RS04655 and BWI76_RS04660 are separated by 10 nucleotides
BWI76_RS04650: BWI76_RS04650 - MFS transporter, at 968,531 to 969,745
_RS04650
BWI76_RS04655: BWI76_RS04655 - shikimate dehydrogenase, at 969,757 to 970,620
_RS04655
BWI76_RS04660: BWI76_RS04660 - 2,4-dienoyl-CoA reductase, at 970,631 to 972,664
_RS04660
Position (kb)
969
970
971 Strain fitness (log2 ratio)
-3
-2
-1
0
1 at 968.770 kb on - strand, within BWI76_RS04650 at 968.794 kb on - strand, within BWI76_RS04650 at 968.818 kb on - strand, within BWI76_RS04650 at 968.990 kb on - strand, within BWI76_RS04650 at 969.100 kb on + strand, within BWI76_RS04650 at 969.100 kb on + strand, within BWI76_RS04650 at 969.108 kb on - strand, within BWI76_RS04650 at 969.137 kb on - strand, within BWI76_RS04650 at 969.272 kb on + strand, within BWI76_RS04650 at 969.347 kb on - strand, within BWI76_RS04650 at 969.347 kb on - strand, within BWI76_RS04650 at 969.347 kb on - strand, within BWI76_RS04650 at 969.352 kb on + strand, within BWI76_RS04650 at 969.662 kb on - strand at 969.778 kb on - strand at 969.825 kb on + strand at 969.825 kb on + strand at 969.921 kb on + strand, within BWI76_RS04655 at 970.025 kb on - strand at 970.287 kb on - strand, within BWI76_RS04655 at 970.356 kb on + strand, within BWI76_RS04655 at 970.458 kb on - strand, within BWI76_RS04655 at 970.545 kb on - strand at 970.798 kb on + strand at 970.834 kb on + strand at 970.842 kb on - strand, within BWI76_RS04660 at 970.921 kb on + strand, within BWI76_RS04660 at 970.932 kb on + strand, within BWI76_RS04660 at 970.940 kb on - strand, within BWI76_RS04660 at 970.999 kb on + strand, within BWI76_RS04660 at 971.391 kb on - strand, within BWI76_RS04660 at 971.511 kb on - strand, within BWI76_RS04660 at 971.587 kb on - strand, within BWI76_RS04660
Per-strain Table
Position Strand Gene LocusTag Fraction RCH2_defined_noCarbon with Mixed culture; Ralstonia sp. UNC404CL21Col 3:1 starting OD to Klebsiella and Glu_DLac_Acetate_mix 1 x remove 968,770 - BWI76_RS04650 0.20 +0.2 968,794 - BWI76_RS04650 0.22 -0.6 968,818 - BWI76_RS04650 0.24 +0.6 968,990 - BWI76_RS04650 0.38 -1.6 969,100 + BWI76_RS04650 0.47 -1.3 969,100 + BWI76_RS04650 0.47 +0.6 969,108 - BWI76_RS04650 0.47 +0.6 969,137 - BWI76_RS04650 0.50 +0.1 969,272 + BWI76_RS04650 0.61 +1.6 969,347 - BWI76_RS04650 0.67 +0.6 969,347 - BWI76_RS04650 0.67 +1.0 969,347 - BWI76_RS04650 0.67 -0.6 969,352 + BWI76_RS04650 0.68 -0.2 969,662 - -0.6 969,778 - -0.7 969,825 + -1.0 969,825 + +0.6 969,921 + BWI76_RS04655 0.19 +0.6 970,025 - +1.0 970,287 - BWI76_RS04655 0.61 +1.4 970,356 + BWI76_RS04655 0.69 +1.0 970,458 - BWI76_RS04655 0.81 -2.0 970,545 - +1.6 970,798 + +0.6 970,834 + +0.4 970,842 - BWI76_RS04660 0.10 -0.2 970,921 + BWI76_RS04660 0.14 -0.3 970,932 + BWI76_RS04660 0.15 -1.6 970,940 - BWI76_RS04660 0.15 +0.3 970,999 + BWI76_RS04660 0.18 -0.5 971,391 - BWI76_RS04660 0.37 -3.1 971,511 - BWI76_RS04660 0.43 -0.6 971,587 - BWI76_RS04660 0.47 -2.3
Or see this region's nucleotide sequence