Experiment: RCH2_defined_noCarbon with Mixed culture; Ralstonia sp. UNC404CL21Col 3:1 starting OD to Klebsiella and Glu_DLac_Acetate_mix 1 x
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS04550 and BWI76_RS04555 are separated by 323 nucleotides BWI76_RS04555 and BWI76_RS04560 are separated by 168 nucleotides BWI76_RS04560 and BWI76_RS04565 are separated by 81 nucleotides
BWI76_RS04550: BWI76_RS04550 - two-component sensor kinase, at 942,017 to 943,642
_RS04550
BWI76_RS04555: BWI76_RS04555 - citrate/acetate antiporter, at 943,966 to 945,321
_RS04555
BWI76_RS04560: BWI76_RS04560 - holo-ACP synthase CitX, at 945,490 to 946,029
_RS04560
BWI76_RS04565: BWI76_RS04565 - bifunctional tRNA pseudouridine(32) synthase/ribosomal large subunit pseudouridine synthase RluA, at 946,111 to 946,770
_RS04565
Position (kb)
943
944
945
946 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2
3 at 943.022 kb on - strand, within BWI76_RS04550 at 943.082 kb on + strand, within BWI76_RS04550 at 943.243 kb on + strand, within BWI76_RS04550 at 943.280 kb on + strand, within BWI76_RS04550 at 943.331 kb on + strand, within BWI76_RS04550 at 943.336 kb on + strand, within BWI76_RS04550 at 943.360 kb on + strand, within BWI76_RS04550 at 943.406 kb on - strand, within BWI76_RS04550 at 943.455 kb on + strand, within BWI76_RS04550 at 943.537 kb on + strand at 943.537 kb on + strand at 943.537 kb on + strand at 943.566 kb on + strand at 943.566 kb on + strand at 943.566 kb on + strand at 943.566 kb on + strand at 943.566 kb on + strand at 943.567 kb on - strand at 943.574 kb on - strand at 943.574 kb on - strand at 943.574 kb on - strand at 943.574 kb on - strand at 943.574 kb on - strand at 943.618 kb on + strand at 943.625 kb on - strand at 943.645 kb on + strand at 943.645 kb on + strand at 943.645 kb on + strand at 943.645 kb on + strand at 943.645 kb on + strand at 943.645 kb on + strand at 943.653 kb on - strand at 943.653 kb on - strand at 943.653 kb on - strand at 943.653 kb on - strand at 943.653 kb on - strand at 943.657 kb on + strand at 943.657 kb on + strand at 943.657 kb on + strand at 943.657 kb on + strand at 943.657 kb on + strand at 943.657 kb on + strand at 943.665 kb on - strand at 943.665 kb on - strand at 943.665 kb on - strand at 943.665 kb on - strand at 943.665 kb on - strand at 943.703 kb on + strand at 943.703 kb on + strand at 943.708 kb on + strand at 943.710 kb on + strand at 943.713 kb on + strand at 943.716 kb on - strand at 943.718 kb on + strand at 943.733 kb on + strand at 943.733 kb on + strand at 943.733 kb on + strand at 943.734 kb on + strand at 943.741 kb on - strand at 943.741 kb on - strand at 943.741 kb on - strand at 943.748 kb on + strand at 943.774 kb on - strand at 943.776 kb on + strand at 943.823 kb on + strand at 943.862 kb on - strand at 943.881 kb on - strand at 943.902 kb on + strand at 943.905 kb on + strand at 943.905 kb on + strand at 943.907 kb on + strand at 943.907 kb on + strand at 943.907 kb on + strand at 943.907 kb on + strand at 943.907 kb on + strand at 943.907 kb on + strand at 943.915 kb on - strand at 943.915 kb on - strand at 943.915 kb on - strand at 943.915 kb on - strand at 943.915 kb on - strand at 943.915 kb on - strand at 943.915 kb on - strand at 943.917 kb on + strand at 943.917 kb on + strand at 943.917 kb on + strand at 943.925 kb on - strand at 943.925 kb on - strand at 943.926 kb on + strand at 943.926 kb on + strand at 943.926 kb on + strand at 943.934 kb on - strand at 943.934 kb on - strand at 943.959 kb on - strand at 943.973 kb on + strand at 943.973 kb on + strand at 943.973 kb on + strand at 943.988 kb on - strand at 943.990 kb on - strand at 944.042 kb on + strand at 944.060 kb on + strand at 944.072 kb on + strand at 944.180 kb on + strand, within BWI76_RS04555 at 944.240 kb on - strand, within BWI76_RS04555 at 944.240 kb on - strand, within BWI76_RS04555 at 944.250 kb on - strand, within BWI76_RS04555 at 944.267 kb on + strand, within BWI76_RS04555 at 944.288 kb on + strand, within BWI76_RS04555 at 944.390 kb on + strand, within BWI76_RS04555 at 944.399 kb on + strand, within BWI76_RS04555 at 944.409 kb on + strand, within BWI76_RS04555 at 944.474 kb on + strand, within BWI76_RS04555 at 944.665 kb on - strand, within BWI76_RS04555 at 944.872 kb on - strand, within BWI76_RS04555 at 944.905 kb on + strand, within BWI76_RS04555 at 944.910 kb on + strand, within BWI76_RS04555 at 944.910 kb on + strand, within BWI76_RS04555 at 944.919 kb on + strand, within BWI76_RS04555 at 944.970 kb on - strand, within BWI76_RS04555 at 944.983 kb on + strand, within BWI76_RS04555 at 944.991 kb on - strand, within BWI76_RS04555 at 944.991 kb on - strand, within BWI76_RS04555 at 944.991 kb on - strand, within BWI76_RS04555 at 945.058 kb on - strand, within BWI76_RS04555 at 945.058 kb on - strand, within BWI76_RS04555 at 945.072 kb on + strand, within BWI76_RS04555 at 945.187 kb on - strand at 945.525 kb on - strand at 945.626 kb on - strand, within BWI76_RS04560 at 945.644 kb on + strand, within BWI76_RS04560 at 945.773 kb on - strand, within BWI76_RS04560 at 945.809 kb on - strand, within BWI76_RS04560 at 946.053 kb on - strand at 946.098 kb on - strand at 946.108 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction RCH2_defined_noCarbon with Mixed culture; Ralstonia sp. UNC404CL21Col 3:1 starting OD to Klebsiella and Glu_DLac_Acetate_mix 1 x remove 943,022 - BWI76_RS04550 0.62 +1.7 943,082 + BWI76_RS04550 0.65 +1.0 943,243 + BWI76_RS04550 0.75 +0.8 943,280 + BWI76_RS04550 0.78 +0.2 943,331 + BWI76_RS04550 0.81 -0.7 943,336 + BWI76_RS04550 0.81 -1.5 943,360 + BWI76_RS04550 0.83 +0.5 943,406 - BWI76_RS04550 0.85 +0.4 943,455 + BWI76_RS04550 0.88 +1.1 943,537 + -0.5 943,537 + +0.5 943,537 + -2.4 943,566 + +0.5 943,566 + -0.6 943,566 + -1.6 943,566 + +0.4 943,566 + +2.2 943,567 - +0.6 943,574 - -1.0 943,574 - +0.1 943,574 - -0.4 943,574 - +0.1 943,574 - -0.1 943,618 + -1.0 943,625 - -1.1 943,645 + +1.7 943,645 + +0.5 943,645 + -1.4 943,645 + -2.7 943,645 + -0.2 943,645 + +0.9 943,653 - -0.6 943,653 - -2.0 943,653 - +0.1 943,653 - -0.8 943,653 - -0.6 943,657 + -0.9 943,657 + -0.6 943,657 + -2.2 943,657 + -1.3 943,657 + -0.6 943,657 + +0.1 943,665 - -2.0 943,665 - -0.0 943,665 - +0.2 943,665 - -0.3 943,665 - +0.1 943,703 + -0.6 943,703 + -1.3 943,708 + +1.1 943,710 + +0.8 943,713 + -0.3 943,716 - +0.2 943,718 + -2.2 943,733 + -3.2 943,733 + +0.7 943,733 + -0.6 943,734 + -3.4 943,741 - -2.8 943,741 - -3.5 943,741 - +1.0 943,748 + -0.9 943,774 - +1.3 943,776 + -0.9 943,823 + -1.0 943,862 - +1.2 943,881 - -1.0 943,902 + -2.2 943,905 + -0.4 943,905 + -1.5 943,907 + -0.3 943,907 + +0.8 943,907 + +1.1 943,907 + -3.4 943,907 + +0.9 943,907 + +0.4 943,915 - +0.4 943,915 - +0.3 943,915 - +0.9 943,915 - -1.3 943,915 - -0.6 943,915 - +1.3 943,915 - +0.7 943,917 + +0.9 943,917 + -0.2 943,917 + -0.2 943,925 - -0.2 943,925 - -2.6 943,926 + +0.1 943,926 + +1.1 943,926 + -1.7 943,934 - +0.4 943,934 - +1.6 943,959 - -2.9 943,973 + -1.0 943,973 + -0.2 943,973 + +0.2 943,988 - -3.4 943,990 - -2.7 944,042 + -0.2 944,060 + -1.6 944,072 + +3.0 944,180 + BWI76_RS04555 0.16 +0.6 944,240 - BWI76_RS04555 0.20 +1.0 944,240 - BWI76_RS04555 0.20 -2.0 944,250 - BWI76_RS04555 0.21 +1.1 944,267 + BWI76_RS04555 0.22 +0.6 944,288 + BWI76_RS04555 0.24 +0.6 944,390 + BWI76_RS04555 0.31 -0.5 944,399 + BWI76_RS04555 0.32 +0.9 944,409 + BWI76_RS04555 0.33 +0.5 944,474 + BWI76_RS04555 0.37 +2.5 944,665 - BWI76_RS04555 0.52 +0.2 944,872 - BWI76_RS04555 0.67 +0.1 944,905 + BWI76_RS04555 0.69 +0.3 944,910 + BWI76_RS04555 0.70 +1.2 944,910 + BWI76_RS04555 0.70 -1.8 944,919 + BWI76_RS04555 0.70 +0.1 944,970 - BWI76_RS04555 0.74 -4.1 944,983 + BWI76_RS04555 0.75 +0.6 944,991 - BWI76_RS04555 0.76 -3.5 944,991 - BWI76_RS04555 0.76 -2.0 944,991 - BWI76_RS04555 0.76 +0.2 945,058 - BWI76_RS04555 0.81 +0.3 945,058 - BWI76_RS04555 0.81 -0.1 945,072 + BWI76_RS04555 0.82 +1.4 945,187 - +0.9 945,525 - +0.1 945,626 - BWI76_RS04560 0.25 -0.6 945,644 + BWI76_RS04560 0.29 -2.5 945,773 - BWI76_RS04560 0.52 +1.4 945,809 - BWI76_RS04560 0.59 +2.2 946,053 - -1.8 946,098 - -0.9 946,108 - +0.2
Or see this region's nucleotide sequence