Experiment: RCH2_defined_noCarbon with Mixed culture; Ralstonia sp. UNC404CL21Col 3:1 starting OD to Klebsiella and Glu_DLac_Acetate_mix 1 x
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS04245 and BWI76_RS04250 are separated by 1 nucleotides BWI76_RS04250 and BWI76_RS04255 are separated by 3 nucleotides
BWI76_RS04245: BWI76_RS04245 - bifunctional aspartate kinase/homoserine dehydrogenase I, at 872,525 to 874,987
_RS04245
BWI76_RS04250: BWI76_RS04250 - homoserine kinase, at 874,989 to 875,918
_RS04250
BWI76_RS04255: BWI76_RS04255 - threonine synthase, at 875,922 to 877,202
_RS04255
Position (kb) 874
875
876 Strain fitness (log2 ratio) -6
-5
-4
-3
-2
-1
0
1 at 874.039 kb on + strand, within BWI76_RS04245 at 874.039 kb on + strand, within BWI76_RS04245 at 874.082 kb on - strand, within BWI76_RS04245 at 874.282 kb on + strand, within BWI76_RS04245 at 874.290 kb on + strand, within BWI76_RS04245 at 874.435 kb on - strand, within BWI76_RS04245 at 874.480 kb on - strand, within BWI76_RS04245 at 874.490 kb on - strand, within BWI76_RS04245 at 874.654 kb on - strand, within BWI76_RS04245 at 874.675 kb on - strand, within BWI76_RS04245 at 874.905 kb on + strand at 874.949 kb on + strand at 875.101 kb on - strand, within BWI76_RS04250 at 875.266 kb on + strand, within BWI76_RS04250 at 875.274 kb on - strand, within BWI76_RS04250 at 875.422 kb on - strand, within BWI76_RS04250 at 875.516 kb on - strand, within BWI76_RS04250 at 875.539 kb on - strand at 875.587 kb on + strand, within BWI76_RS04250 at 875.595 kb on - strand, within BWI76_RS04250 at 875.599 kb on + strand, within BWI76_RS04250 at 875.615 kb on - strand, within BWI76_RS04250 at 875.846 kb on - strand at 875.962 kb on + strand at 875.965 kb on - strand at 875.970 kb on - strand at 875.987 kb on - strand at 876.169 kb on - strand, within BWI76_RS04255 at 876.213 kb on - strand, within BWI76_RS04255 at 876.223 kb on + strand, within BWI76_RS04255 at 876.398 kb on + strand, within BWI76_RS04255 at 876.428 kb on + strand, within BWI76_RS04255 at 876.620 kb on + strand, within BWI76_RS04255 at 876.620 kb on + strand, within BWI76_RS04255 at 876.644 kb on + strand, within BWI76_RS04255 at 876.644 kb on + strand, within BWI76_RS04255 at 876.812 kb on + strand, within BWI76_RS04255 at 876.812 kb on + strand, within BWI76_RS04255 at 876.820 kb on - strand, within BWI76_RS04255 at 876.820 kb on - strand, within BWI76_RS04255
Per-strain Table
Position Strand Gene LocusTag Fraction RCH2_defined_noCarbon with Mixed culture; Ralstonia sp. UNC404CL21Col 3:1 starting OD to Klebsiella and Glu_DLac_Acetate_mix 1 x remove 874,039 + BWI76_RS04245 0.61 -0.7 874,039 + BWI76_RS04245 0.61 -5.2 874,082 - BWI76_RS04245 0.63 -2.4 874,282 + BWI76_RS04245 0.71 -2.3 874,290 + BWI76_RS04245 0.72 -3.4 874,435 - BWI76_RS04245 0.78 -5.4 874,480 - BWI76_RS04245 0.79 -5.5 874,490 - BWI76_RS04245 0.80 -3.1 874,654 - BWI76_RS04245 0.86 -4.9 874,675 - BWI76_RS04245 0.87 -5.4 874,905 + -1.8 874,949 + -3.4 875,101 - BWI76_RS04250 0.12 -5.2 875,266 + BWI76_RS04250 0.30 -3.5 875,274 - BWI76_RS04250 0.31 -5.4 875,422 - BWI76_RS04250 0.47 -5.7 875,516 - BWI76_RS04250 0.57 -4.8 875,539 - +1.0 875,587 + BWI76_RS04250 0.64 -1.6 875,595 - BWI76_RS04250 0.65 -3.0 875,599 + BWI76_RS04250 0.66 -5.5 875,615 - BWI76_RS04250 0.67 -1.3 875,846 - -3.4 875,962 + -2.8 875,965 - +0.0 875,970 - -3.6 875,987 - -5.1 876,169 - BWI76_RS04255 0.19 -5.3 876,213 - BWI76_RS04255 0.23 -3.4 876,223 + BWI76_RS04255 0.23 -6.2 876,398 + BWI76_RS04255 0.37 -2.7 876,428 + BWI76_RS04255 0.40 +1.0 876,620 + BWI76_RS04255 0.54 -4.4 876,620 + BWI76_RS04255 0.54 -2.1 876,644 + BWI76_RS04255 0.56 -1.8 876,644 + BWI76_RS04255 0.56 +0.0 876,812 + BWI76_RS04255 0.69 -3.2 876,812 + BWI76_RS04255 0.69 -2.6 876,820 - BWI76_RS04255 0.70 -2.4 876,820 - BWI76_RS04255 0.70 -4.0
Or see this region's nucleotide sequence