Experiment: RCH2_defined_noCarbon with Mixed culture; Ralstonia sp. UNC404CL21Col 3:1 starting OD to Klebsiella and Glu_DLac_Acetate_mix 1 x
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS04190 and BWI76_RS04195 overlap by 20 nucleotides BWI76_RS04195 and BWI76_RS04200 are separated by 114 nucleotides BWI76_RS04200 and BWI76_RS04205 overlap by 4 nucleotides
BWI76_RS04190: BWI76_RS04190 - Trp operon repressor, at 863,384 to 863,716
_RS04190
BWI76_RS04195: BWI76_RS04195 - non-canonical purine NTP phosphatase, at 863,697 to 864,218
_RS04195
BWI76_RS04200: BWI76_RS04200 - phosphoglycerate mutase GpmB, at 864,333 to 864,980
_RS04200
BWI76_RS04205: BWI76_RS04205 - right oriC-binding transcriptional activator, at 864,977 to 865,846
_RS04205
Position (kb) 864
865 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3 at 864.304 kb on + strand at 864.305 kb on - strand at 864.313 kb on - strand at 864.581 kb on + strand, within BWI76_RS04200 at 864.589 kb on - strand, within BWI76_RS04200 at 864.892 kb on + strand, within BWI76_RS04200 at 865.206 kb on + strand, within BWI76_RS04205 at 865.409 kb on + strand, within BWI76_RS04205 at 865.410 kb on + strand, within BWI76_RS04205 at 865.416 kb on - strand, within BWI76_RS04205 at 865.420 kb on + strand, within BWI76_RS04205 at 865.435 kb on + strand, within BWI76_RS04205 at 865.538 kb on + strand, within BWI76_RS04205 at 865.551 kb on - strand, within BWI76_RS04205 at 865.653 kb on - strand, within BWI76_RS04205 at 865.759 kb on - strand, within BWI76_RS04205 at 865.767 kb on + strand at 865.800 kb on + strand at 865.809 kb on + strand at 865.811 kb on + strand at 865.811 kb on + strand at 865.811 kb on + strand at 865.811 kb on + strand at 865.811 kb on + strand at 865.811 kb on + strand at 865.811 kb on + strand at 865.819 kb on - strand at 865.819 kb on - strand at 865.819 kb on - strand at 865.819 kb on - strand at 865.819 kb on - strand at 865.859 kb on + strand at 865.859 kb on + strand at 865.859 kb on + strand at 865.866 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction RCH2_defined_noCarbon with Mixed culture; Ralstonia sp. UNC404CL21Col 3:1 starting OD to Klebsiella and Glu_DLac_Acetate_mix 1 x remove 864,304 + +0.6 864,305 - +0.0 864,313 - +0.0 864,581 + BWI76_RS04200 0.38 +0.7 864,589 - BWI76_RS04200 0.40 +3.0 864,892 + BWI76_RS04200 0.86 +0.1 865,206 + BWI76_RS04205 0.26 +0.2 865,409 + BWI76_RS04205 0.50 -0.1 865,410 + BWI76_RS04205 0.50 +0.9 865,416 - BWI76_RS04205 0.50 +1.6 865,420 + BWI76_RS04205 0.51 -3.2 865,435 + BWI76_RS04205 0.53 -0.6 865,538 + BWI76_RS04205 0.64 +0.5 865,551 - BWI76_RS04205 0.66 -0.1 865,653 - BWI76_RS04205 0.78 +0.3 865,759 - BWI76_RS04205 0.90 +1.1 865,767 + -1.0 865,800 + +0.4 865,809 + +0.3 865,811 + +0.8 865,811 + +0.8 865,811 + -1.8 865,811 + -1.0 865,811 + -0.1 865,811 + -0.4 865,811 + +0.4 865,819 - -2.9 865,819 - +0.3 865,819 - +1.0 865,819 - +1.0 865,819 - -2.3 865,859 + +1.9 865,859 + +1.2 865,859 + +1.3 865,866 + +0.9
Or see this region's nucleotide sequence