Experiment: RCH2_defined_noCarbon with Mixed culture; Ralstonia sp. UNC404CL21Col 3:1 starting OD to Klebsiella and Glu_DLac_Acetate_mix 1 x
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS04130 and BWI76_RS04135 are separated by 77 nucleotides BWI76_RS04135 and BWI76_RS04140 are separated by 52 nucleotides
BWI76_RS04130: BWI76_RS04130 - 2-deoxyribose-5-phosphate aldolase, at 849,568 to 850,347
_RS04130
BWI76_RS04135: BWI76_RS04135 - thymidine phosphorylase, at 850,425 to 851,747
_RS04135
BWI76_RS04140: BWI76_RS04140 - phosphopentomutase, at 851,800 to 853,023
_RS04140
Position (kb)
850
851
852 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1 at 849.483 kb on - strand at 849.607 kb on - strand at 849.727 kb on - strand, within BWI76_RS04130 at 849.761 kb on + strand, within BWI76_RS04130 at 849.776 kb on + strand, within BWI76_RS04130 at 849.828 kb on + strand, within BWI76_RS04130 at 849.870 kb on - strand, within BWI76_RS04130 at 849.928 kb on - strand, within BWI76_RS04130 at 849.933 kb on - strand, within BWI76_RS04130 at 850.241 kb on - strand, within BWI76_RS04130 at 850.396 kb on + strand at 850.652 kb on - strand, within BWI76_RS04135 at 850.836 kb on + strand, within BWI76_RS04135 at 851.062 kb on + strand, within BWI76_RS04135 at 851.062 kb on + strand, within BWI76_RS04135 at 851.077 kb on - strand, within BWI76_RS04135 at 851.136 kb on + strand at 851.331 kb on + strand, within BWI76_RS04135 at 851.339 kb on - strand, within BWI76_RS04135 at 851.339 kb on - strand, within BWI76_RS04135 at 851.661 kb on - strand at 851.663 kb on + strand at 851.752 kb on + strand at 851.755 kb on - strand at 851.760 kb on - strand at 851.896 kb on - strand at 851.972 kb on + strand, within BWI76_RS04140 at 851.980 kb on - strand, within BWI76_RS04140 at 851.990 kb on - strand, within BWI76_RS04140 at 852.095 kb on + strand, within BWI76_RS04140 at 852.195 kb on + strand, within BWI76_RS04140 at 852.378 kb on + strand, within BWI76_RS04140 at 852.585 kb on + strand, within BWI76_RS04140
Per-strain Table
Position Strand Gene LocusTag Fraction RCH2_defined_noCarbon with Mixed culture; Ralstonia sp. UNC404CL21Col 3:1 starting OD to Klebsiella and Glu_DLac_Acetate_mix 1 x remove 849,483 - -1.3 849,607 - +0.9 849,727 - BWI76_RS04130 0.20 +0.4 849,761 + BWI76_RS04130 0.25 +0.0 849,776 + BWI76_RS04130 0.27 +0.4 849,828 + BWI76_RS04130 0.33 +0.6 849,870 - BWI76_RS04130 0.39 -0.7 849,928 - BWI76_RS04130 0.46 +0.5 849,933 - BWI76_RS04130 0.47 +1.0 850,241 - BWI76_RS04130 0.86 +0.3 850,396 + +1.1 850,652 - BWI76_RS04135 0.17 +0.3 850,836 + BWI76_RS04135 0.31 -0.6 851,062 + BWI76_RS04135 0.48 -1.6 851,062 + BWI76_RS04135 0.48 -3.0 851,077 - BWI76_RS04135 0.49 -1.0 851,136 + -0.6 851,331 + BWI76_RS04135 0.68 +0.2 851,339 - BWI76_RS04135 0.69 -2.2 851,339 - BWI76_RS04135 0.69 -3.8 851,661 - -0.3 851,663 + -1.2 851,752 + -0.3 851,755 - +0.6 851,760 - +1.3 851,896 - -1.4 851,972 + BWI76_RS04140 0.14 +0.1 851,980 - BWI76_RS04140 0.15 -1.3 851,990 - BWI76_RS04140 0.16 -2.3 852,095 + BWI76_RS04140 0.24 +0.4 852,195 + BWI76_RS04140 0.32 -1.8 852,378 + BWI76_RS04140 0.47 -1.8 852,585 + BWI76_RS04140 0.64 -0.7
Or see this region's nucleotide sequence