Experiment: RCH2_defined_noCarbon with Mixed culture; Ralstonia sp. UNC404CL21Col 3:1 starting OD to Klebsiella and Glu_DLac_Acetate_mix 1 x
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS03865 and BWI76_RS03870 overlap by 4 nucleotides BWI76_RS03870 and BWI76_RS03875 overlap by 4 nucleotides BWI76_RS03875 and BWI76_RS03880 are separated by 284 nucleotides BWI76_RS03880 and BWI76_RS03885 are separated by 118 nucleotides
BWI76_RS03865: BWI76_RS03865 - 5-carboxymethyl-2-hydroxymuconate semialdehyde dehydrogenase, at 799,174 to 800,640
_RS03865
BWI76_RS03870: BWI76_RS03870 - 2-hydroxyhepta-2,4-diene-1,7-dioate isomerase, at 800,637 to 801,401
_RS03870
BWI76_RS03875: BWI76_RS03875 - 2-hydroxyhepta-2,4-diene-1,7-dioate isomerase, at 801,398 to 802,030
_RS03875
BWI76_RS03880: BWI76_RS03880 - homoprotocatechuate degradation operon regulator, HpaR, at 802,315 to 802,752
_RS03880
BWI76_RS03885: BWI76_RS03885 - hypothetical protein, at 802,871 to 804,085
_RS03885
Position (kb) 801
802
803 Strain fitness (log2 ratio) -2
-1
0
1
2 at 800.579 kb on - strand at 800.888 kb on - strand, within BWI76_RS03870 at 801.013 kb on + strand, within BWI76_RS03870 at 801.133 kb on - strand, within BWI76_RS03870 at 801.172 kb on - strand, within BWI76_RS03870 at 801.244 kb on - strand, within BWI76_RS03870 at 801.246 kb on + strand, within BWI76_RS03870 at 801.249 kb on - strand, within BWI76_RS03870 at 801.255 kb on - strand, within BWI76_RS03870 at 801.351 kb on + strand at 801.494 kb on + strand, within BWI76_RS03875 at 801.651 kb on + strand, within BWI76_RS03875 at 801.950 kb on - strand, within BWI76_RS03875 at 801.957 kb on + strand, within BWI76_RS03875 at 802.313 kb on - strand at 802.514 kb on + strand, within BWI76_RS03880 at 802.532 kb on + strand, within BWI76_RS03880 at 802.540 kb on - strand, within BWI76_RS03880 at 802.741 kb on + strand at 802.891 kb on - strand at 802.899 kb on + strand at 802.900 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction RCH2_defined_noCarbon with Mixed culture; Ralstonia sp. UNC404CL21Col 3:1 starting OD to Klebsiella and Glu_DLac_Acetate_mix 1 x remove 800,579 - +0.6 800,888 - BWI76_RS03870 0.33 +1.1 801,013 + BWI76_RS03870 0.49 +0.4 801,133 - BWI76_RS03870 0.65 +0.7 801,172 - BWI76_RS03870 0.70 +0.9 801,244 - BWI76_RS03870 0.79 +1.7 801,246 + BWI76_RS03870 0.80 +2.1 801,249 - BWI76_RS03870 0.80 +0.3 801,255 - BWI76_RS03870 0.81 +0.8 801,351 + +0.5 801,494 + BWI76_RS03875 0.15 +1.1 801,651 + BWI76_RS03875 0.40 +1.0 801,950 - BWI76_RS03875 0.87 -0.2 801,957 + BWI76_RS03875 0.88 +1.3 802,313 - +2.7 802,514 + BWI76_RS03880 0.45 -0.9 802,532 + BWI76_RS03880 0.50 +0.4 802,540 - BWI76_RS03880 0.51 +0.4 802,741 + +0.4 802,891 - +0.5 802,899 + -2.6 802,900 + -2.4
Or see this region's nucleotide sequence