Experiment: RCH2_defined_noCarbon with Mixed culture; Ralstonia sp. UNC404CL21Col 3:1 starting OD to Klebsiella and Glu_DLac_Acetate_mix 1 x
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS03640 and BWI76_RS03645 overlap by 4 nucleotides BWI76_RS03645 and BWI76_RS03650 are separated by 451 nucleotides BWI76_RS03650 and BWI76_RS03655 overlap by 2 nucleotides BWI76_RS03655 and BWI76_RS03660 overlap by 29 nucleotides
BWI76_RS03640: BWI76_RS03640 - amino acid permease-associated protein, at 751,683 to 753,026
_RS03640
BWI76_RS03645: BWI76_RS03645 - hypothetical protein, at 753,023 to 753,934
_RS03645
BWI76_RS03650: BWI76_RS03650 - endoribonuclease SymE, at 754,386 to 754,721
_RS03650
BWI76_RS03655: BWI76_RS03655 - hypothetical protein, at 754,720 to 754,920
_RS03655
BWI76_RS03660: BWI76_RS03660 - hypothetical protein, at 754,892 to 756,757
_RS03660
Position (kb)
753
754 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2
3
4 at 752.242 kb on + strand, within BWI76_RS03640 at 752.243 kb on - strand, within BWI76_RS03640 at 752.243 kb on - strand, within BWI76_RS03640 at 752.366 kb on - strand, within BWI76_RS03640 at 752.412 kb on - strand, within BWI76_RS03640 at 752.460 kb on - strand, within BWI76_RS03640 at 752.582 kb on + strand, within BWI76_RS03640 at 752.590 kb on - strand, within BWI76_RS03640 at 752.597 kb on + strand, within BWI76_RS03640 at 752.626 kb on - strand, within BWI76_RS03640 at 752.697 kb on - strand, within BWI76_RS03640 at 752.697 kb on - strand, within BWI76_RS03640 at 752.782 kb on - strand, within BWI76_RS03640 at 752.789 kb on - strand, within BWI76_RS03640 at 752.961 kb on - strand at 753.069 kb on + strand at 753.077 kb on - strand at 753.140 kb on + strand, within BWI76_RS03645 at 753.148 kb on - strand, within BWI76_RS03645 at 753.580 kb on + strand, within BWI76_RS03645 at 753.673 kb on - strand, within BWI76_RS03645 at 753.684 kb on - strand, within BWI76_RS03645 at 753.730 kb on + strand, within BWI76_RS03645 at 753.744 kb on + strand, within BWI76_RS03645 at 753.773 kb on - strand, within BWI76_RS03645 at 753.774 kb on - strand, within BWI76_RS03645 at 753.848 kb on - strand at 753.848 kb on - strand at 753.858 kb on - strand at 753.858 kb on - strand at 753.866 kb on - strand at 753.874 kb on - strand at 753.874 kb on - strand at 753.874 kb on - strand at 753.885 kb on + strand at 753.887 kb on - strand at 753.887 kb on - strand at 753.887 kb on - strand at 753.888 kb on - strand at 753.891 kb on + strand at 753.893 kb on - strand at 753.902 kb on + strand at 753.903 kb on + strand at 753.903 kb on + strand at 753.903 kb on + strand at 753.903 kb on + strand at 753.903 kb on + strand at 753.903 kb on + strand at 753.910 kb on - strand at 753.910 kb on - strand at 753.910 kb on - strand at 753.910 kb on - strand at 753.911 kb on - strand at 753.911 kb on - strand at 753.911 kb on - strand at 753.911 kb on - strand at 753.911 kb on - strand at 753.911 kb on - strand at 753.911 kb on - strand at 753.911 kb on - strand at 753.911 kb on - strand at 753.937 kb on + strand at 753.954 kb on - strand at 753.956 kb on - strand at 753.987 kb on + strand at 754.018 kb on - strand at 754.093 kb on - strand at 754.170 kb on + strand at 754.177 kb on + strand at 754.177 kb on + strand at 754.177 kb on + strand at 754.185 kb on + strand at 754.198 kb on + strand at 754.198 kb on + strand at 754.203 kb on + strand at 754.211 kb on + strand at 754.219 kb on - strand at 754.219 kb on - strand at 754.243 kb on + strand at 754.243 kb on + strand at 754.258 kb on - strand at 754.261 kb on + strand at 754.261 kb on + strand at 754.261 kb on + strand at 754.261 kb on + strand at 754.261 kb on + strand at 754.261 kb on + strand at 754.261 kb on + strand at 754.261 kb on + strand at 754.261 kb on + strand at 754.261 kb on + strand at 754.261 kb on + strand at 754.261 kb on + strand at 754.261 kb on + strand at 754.261 kb on + strand at 754.261 kb on + strand at 754.261 kb on + strand at 754.261 kb on + strand at 754.262 kb on + strand at 754.262 kb on + strand at 754.262 kb on - strand at 754.269 kb on - strand at 754.269 kb on - strand at 754.269 kb on - strand at 754.269 kb on - strand at 754.269 kb on - strand at 754.270 kb on - strand at 754.276 kb on - strand at 754.288 kb on + strand at 754.303 kb on + strand at 754.313 kb on + strand at 754.320 kb on - strand at 754.320 kb on - strand at 754.320 kb on - strand at 754.333 kb on + strand at 754.351 kb on + strand at 754.351 kb on + strand at 754.351 kb on + strand at 754.351 kb on + strand at 754.354 kb on - strand at 754.354 kb on - strand at 754.356 kb on + strand at 754.359 kb on - strand at 754.359 kb on - strand at 754.359 kb on - strand at 754.361 kb on + strand at 754.361 kb on + strand at 754.361 kb on + strand at 754.361 kb on + strand at 754.364 kb on - strand at 754.364 kb on - strand at 754.392 kb on - strand at 754.394 kb on + strand at 754.394 kb on + strand at 754.400 kb on - strand at 754.402 kb on - strand at 754.402 kb on - strand at 754.402 kb on - strand at 754.402 kb on - strand at 754.407 kb on - strand at 754.422 kb on + strand, within BWI76_RS03650 at 754.422 kb on + strand, within BWI76_RS03650 at 754.430 kb on - strand, within BWI76_RS03650 at 754.430 kb on - strand, within BWI76_RS03650 at 754.430 kb on - strand, within BWI76_RS03650 at 754.430 kb on - strand, within BWI76_RS03650 at 754.430 kb on - strand, within BWI76_RS03650 at 754.439 kb on + strand, within BWI76_RS03650 at 754.439 kb on + strand, within BWI76_RS03650 at 754.488 kb on - strand, within BWI76_RS03650 at 754.488 kb on - strand, within BWI76_RS03650 at 754.498 kb on - strand, within BWI76_RS03650 at 754.520 kb on + strand, within BWI76_RS03650 at 754.520 kb on + strand, within BWI76_RS03650 at 754.520 kb on + strand, within BWI76_RS03650 at 754.523 kb on - strand, within BWI76_RS03650 at 754.523 kb on - strand, within BWI76_RS03650 at 754.523 kb on - strand, within BWI76_RS03650 at 754.527 kb on + strand, within BWI76_RS03650 at 754.528 kb on - strand, within BWI76_RS03650 at 754.528 kb on - strand, within BWI76_RS03650 at 754.528 kb on - strand, within BWI76_RS03650 at 754.535 kb on - strand, within BWI76_RS03650 at 754.610 kb on + strand, within BWI76_RS03650 at 754.632 kb on - strand, within BWI76_RS03650 at 754.632 kb on - strand, within BWI76_RS03650 at 754.649 kb on - strand, within BWI76_RS03650 at 754.655 kb on - strand, within BWI76_RS03650 at 754.679 kb on + strand, within BWI76_RS03650 at 754.679 kb on + strand, within BWI76_RS03650 at 754.679 kb on + strand, within BWI76_RS03650 at 754.687 kb on - strand, within BWI76_RS03650 at 754.687 kb on - strand, within BWI76_RS03650 at 754.687 kb on - strand, within BWI76_RS03650 at 754.687 kb on - strand, within BWI76_RS03650 at 754.715 kb on - strand at 754.717 kb on + strand at 754.725 kb on - strand at 754.733 kb on - strand at 754.755 kb on - strand, within BWI76_RS03655 at 754.755 kb on - strand, within BWI76_RS03655 at 754.768 kb on + strand, within BWI76_RS03655 at 754.769 kb on + strand, within BWI76_RS03655 at 754.770 kb on - strand, within BWI76_RS03655 at 754.776 kb on - strand, within BWI76_RS03655 at 754.810 kb on - strand, within BWI76_RS03655 at 754.861 kb on + strand, within BWI76_RS03655 at 754.874 kb on - strand, within BWI76_RS03655 at 754.876 kb on - strand, within BWI76_RS03655 at 754.877 kb on + strand, within BWI76_RS03655
Per-strain Table
Position Strand Gene LocusTag Fraction RCH2_defined_noCarbon with Mixed culture; Ralstonia sp. UNC404CL21Col 3:1 starting OD to Klebsiella and Glu_DLac_Acetate_mix 1 x remove 752,242 + BWI76_RS03640 0.42 +0.5 752,243 - BWI76_RS03640 0.42 -2.6 752,243 - BWI76_RS03640 0.42 -0.2 752,366 - BWI76_RS03640 0.51 +0.9 752,412 - BWI76_RS03640 0.54 -2.6 752,460 - BWI76_RS03640 0.58 +0.2 752,582 + BWI76_RS03640 0.67 -1.9 752,590 - BWI76_RS03640 0.67 -1.7 752,597 + BWI76_RS03640 0.68 -1.7 752,626 - BWI76_RS03640 0.70 +1.1 752,697 - BWI76_RS03640 0.75 +0.7 752,697 - BWI76_RS03640 0.75 +1.1 752,782 - BWI76_RS03640 0.82 +0.1 752,789 - BWI76_RS03640 0.82 +0.4 752,961 - +1.7 753,069 + -1.5 753,077 - +1.1 753,140 + BWI76_RS03645 0.13 +1.7 753,148 - BWI76_RS03645 0.14 +3.8 753,580 + BWI76_RS03645 0.61 +0.8 753,673 - BWI76_RS03645 0.71 -0.2 753,684 - BWI76_RS03645 0.72 +0.3 753,730 + BWI76_RS03645 0.78 -0.0 753,744 + BWI76_RS03645 0.79 -0.1 753,773 - BWI76_RS03645 0.82 -0.7 753,774 - BWI76_RS03645 0.82 +0.7 753,848 - -1.1 753,848 - -0.1 753,858 - -1.2 753,858 - +0.5 753,866 - -0.8 753,874 - -1.9 753,874 - -1.4 753,874 - +0.6 753,885 + +0.1 753,887 - +0.3 753,887 - +1.7 753,887 - -2.3 753,888 - +1.3 753,891 + -2.8 753,893 - +1.1 753,902 + +2.7 753,903 + -0.6 753,903 + +1.3 753,903 + -0.1 753,903 + -0.5 753,903 + +1.1 753,903 + +0.1 753,910 - -0.1 753,910 - +0.8 753,910 - -1.0 753,910 - +0.2 753,911 - +0.4 753,911 - +0.4 753,911 - -0.9 753,911 - -1.9 753,911 - +1.1 753,911 - -1.6 753,911 - +1.0 753,911 - +0.4 753,911 - -1.8 753,937 + -1.2 753,954 - -1.0 753,956 - +2.4 753,987 + -1.7 754,018 - -3.9 754,093 - -3.7 754,170 + +0.9 754,177 + +1.9 754,177 + -1.9 754,177 + -0.8 754,185 + +0.1 754,198 + -1.4 754,198 + +0.5 754,203 + -0.7 754,211 + -1.3 754,219 - -1.5 754,219 - -0.9 754,243 + -2.5 754,243 + -0.1 754,258 - +1.3 754,261 + -0.4 754,261 + -2.6 754,261 + -0.1 754,261 + +1.1 754,261 + -0.4 754,261 + +0.1 754,261 + -1.5 754,261 + +0.1 754,261 + -0.4 754,261 + +0.3 754,261 + +1.1 754,261 + -2.6 754,261 + +0.4 754,261 + +0.7 754,261 + -0.0 754,261 + -0.3 754,261 + +1.6 754,262 + +2.4 754,262 + -0.4 754,262 - +1.1 754,269 - -0.0 754,269 - +0.7 754,269 - +1.1 754,269 - -2.2 754,269 - -0.5 754,270 - -4.0 754,276 - +1.8 754,288 + +0.1 754,303 + -0.6 754,313 + -0.1 754,320 - -1.1 754,320 - -1.9 754,320 - -0.3 754,333 + -2.2 754,351 + -2.5 754,351 + +0.9 754,351 + +0.7 754,351 + -3.8 754,354 - -1.7 754,354 - +0.3 754,356 + -0.1 754,359 - +0.8 754,359 - +0.0 754,359 - -0.9 754,361 + +1.4 754,361 + +0.7 754,361 + -0.9 754,361 + -1.2 754,364 - -1.0 754,364 - +0.9 754,392 - +1.1 754,394 + -3.7 754,394 + +0.3 754,400 - +0.3 754,402 - -0.3 754,402 - -2.4 754,402 - -1.2 754,402 - +0.7 754,407 - +1.1 754,422 + BWI76_RS03650 0.11 -0.0 754,422 + BWI76_RS03650 0.11 +0.3 754,430 - BWI76_RS03650 0.13 -2.8 754,430 - BWI76_RS03650 0.13 -0.0 754,430 - BWI76_RS03650 0.13 +3.4 754,430 - BWI76_RS03650 0.13 -0.2 754,430 - BWI76_RS03650 0.13 -0.9 754,439 + BWI76_RS03650 0.16 +3.3 754,439 + BWI76_RS03650 0.16 -1.3 754,488 - BWI76_RS03650 0.30 +1.1 754,488 - BWI76_RS03650 0.30 -0.9 754,498 - BWI76_RS03650 0.33 -2.4 754,520 + BWI76_RS03650 0.40 +1.2 754,520 + BWI76_RS03650 0.40 -4.6 754,520 + BWI76_RS03650 0.40 -1.6 754,523 - BWI76_RS03650 0.41 +0.4 754,523 - BWI76_RS03650 0.41 +0.5 754,523 - BWI76_RS03650 0.41 +0.5 754,527 + BWI76_RS03650 0.42 -1.7 754,528 - BWI76_RS03650 0.42 +1.0 754,528 - BWI76_RS03650 0.42 -4.1 754,528 - BWI76_RS03650 0.42 +0.1 754,535 - BWI76_RS03650 0.44 +0.1 754,610 + BWI76_RS03650 0.67 -2.5 754,632 - BWI76_RS03650 0.73 -2.9 754,632 - BWI76_RS03650 0.73 -0.3 754,649 - BWI76_RS03650 0.78 +1.4 754,655 - BWI76_RS03650 0.80 +0.4 754,679 + BWI76_RS03650 0.87 -0.7 754,679 + BWI76_RS03650 0.87 +0.6 754,679 + BWI76_RS03650 0.87 -1.5 754,687 - BWI76_RS03650 0.90 -1.7 754,687 - BWI76_RS03650 0.90 -3.1 754,687 - BWI76_RS03650 0.90 +0.5 754,687 - BWI76_RS03650 0.90 -3.2 754,715 - -0.5 754,717 + -1.1 754,725 - -0.1 754,733 - -0.5 754,755 - BWI76_RS03655 0.17 +1.0 754,755 - BWI76_RS03655 0.17 -1.2 754,768 + BWI76_RS03655 0.24 +1.1 754,769 + BWI76_RS03655 0.24 -2.7 754,770 - BWI76_RS03655 0.25 -0.9 754,776 - BWI76_RS03655 0.28 -1.1 754,810 - BWI76_RS03655 0.45 -1.7 754,861 + BWI76_RS03655 0.70 -1.7 754,874 - BWI76_RS03655 0.77 -0.4 754,876 - BWI76_RS03655 0.78 +0.3 754,877 + BWI76_RS03655 0.78 -0.9
Or see this region's nucleotide sequence