Experiment: RCH2_defined_noCarbon with Mixed culture; Ralstonia sp. UNC404CL21Col 3:1 starting OD to Klebsiella and Glu_DLac_Acetate_mix 1 x
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS03225 and BWI76_RS03230 are separated by 48 nucleotides BWI76_RS03230 and BWI76_RS03235 overlap by 4 nucleotides
BWI76_RS03225: BWI76_RS03225 - transcriptional regulator, at 664,764 to 665,570
_RS03225
BWI76_RS03230: BWI76_RS03230 - hypothetical protein, at 665,619 to 666,524
_RS03230
BWI76_RS03235: BWI76_RS03235 - Neopullulanase (EC 3.2.1.135) <small>(from data)</small>, at 666,521 to 668,317
_RS03235
Position (kb) 665
666
667 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1
2
3
4 at 664.627 kb on - strand at 664.649 kb on + strand at 664.649 kb on + strand at 664.658 kb on + strand at 664.660 kb on + strand at 664.660 kb on + strand at 664.660 kb on + strand at 664.660 kb on + strand at 664.660 kb on + strand at 664.660 kb on + strand at 664.660 kb on + strand at 664.660 kb on + strand at 664.660 kb on + strand at 664.660 kb on + strand at 664.666 kb on + strand at 664.668 kb on + strand at 664.668 kb on + strand at 664.668 kb on + strand at 664.668 kb on - strand at 664.668 kb on - strand at 664.668 kb on - strand at 664.668 kb on - strand at 664.668 kb on - strand at 664.668 kb on - strand at 664.668 kb on - strand at 664.668 kb on - strand at 664.668 kb on - strand at 664.668 kb on - strand at 664.668 kb on - strand at 664.668 kb on - strand at 664.668 kb on - strand at 664.668 kb on - strand at 664.668 kb on - strand at 664.668 kb on - strand at 664.671 kb on + strand at 664.679 kb on - strand at 664.679 kb on - strand at 664.681 kb on + strand at 664.690 kb on + strand at 664.696 kb on - strand at 664.700 kb on + strand at 664.719 kb on + strand at 664.738 kb on + strand at 664.738 kb on + strand at 664.748 kb on - strand at 664.748 kb on - strand at 664.793 kb on - strand at 664.795 kb on + strand at 664.798 kb on - strand at 664.820 kb on + strand at 664.836 kb on - strand at 665.006 kb on - strand, within BWI76_RS03225 at 665.091 kb on + strand, within BWI76_RS03225 at 665.100 kb on + strand, within BWI76_RS03225 at 665.100 kb on + strand, within BWI76_RS03225 at 665.108 kb on - strand at 665.108 kb on - strand, within BWI76_RS03225 at 665.130 kb on - strand, within BWI76_RS03225 at 665.130 kb on - strand, within BWI76_RS03225 at 665.155 kb on - strand, within BWI76_RS03225 at 665.155 kb on - strand, within BWI76_RS03225 at 665.155 kb on - strand, within BWI76_RS03225 at 665.155 kb on - strand, within BWI76_RS03225 at 665.158 kb on + strand, within BWI76_RS03225 at 665.190 kb on - strand, within BWI76_RS03225 at 665.261 kb on - strand, within BWI76_RS03225 at 665.261 kb on - strand, within BWI76_RS03225 at 665.275 kb on + strand, within BWI76_RS03225 at 665.279 kb on - strand, within BWI76_RS03225 at 665.307 kb on - strand, within BWI76_RS03225 at 665.443 kb on - strand, within BWI76_RS03225 at 665.445 kb on - strand, within BWI76_RS03225 at 665.476 kb on - strand, within BWI76_RS03225 at 665.477 kb on - strand, within BWI76_RS03225 at 665.513 kb on - strand at 665.513 kb on - strand at 665.513 kb on - strand at 665.513 kb on - strand at 665.513 kb on - strand at 665.543 kb on - strand at 665.543 kb on - strand at 665.543 kb on - strand at 665.559 kb on - strand at 665.579 kb on + strand at 665.580 kb on - strand at 665.580 kb on - strand at 665.587 kb on - strand at 665.630 kb on + strand at 665.630 kb on + strand at 665.630 kb on + strand at 665.630 kb on + strand at 665.630 kb on + strand at 665.630 kb on + strand at 665.630 kb on + strand at 665.630 kb on + strand at 665.630 kb on + strand at 665.630 kb on + strand at 665.630 kb on + strand at 665.630 kb on + strand at 665.630 kb on + strand at 665.630 kb on + strand at 665.630 kb on + strand at 665.631 kb on + strand at 665.635 kb on + strand at 665.635 kb on + strand at 665.638 kb on - strand at 665.638 kb on - strand at 665.638 kb on - strand at 665.638 kb on - strand at 665.638 kb on - strand at 665.638 kb on - strand at 665.638 kb on - strand at 665.638 kb on - strand at 665.638 kb on - strand at 665.638 kb on - strand at 665.638 kb on - strand at 665.638 kb on - strand at 665.638 kb on - strand at 665.640 kb on - strand at 665.645 kb on - strand at 665.648 kb on - strand at 665.648 kb on - strand at 665.648 kb on - strand at 665.662 kb on + strand at 665.677 kb on - strand at 665.739 kb on + strand, within BWI76_RS03230 at 665.753 kb on - strand, within BWI76_RS03230 at 665.753 kb on - strand, within BWI76_RS03230 at 665.775 kb on + strand, within BWI76_RS03230 at 665.805 kb on - strand, within BWI76_RS03230 at 665.812 kb on - strand, within BWI76_RS03230 at 665.841 kb on + strand, within BWI76_RS03230 at 665.843 kb on + strand, within BWI76_RS03230 at 665.848 kb on + strand, within BWI76_RS03230 at 665.848 kb on + strand, within BWI76_RS03230 at 665.848 kb on + strand, within BWI76_RS03230 at 665.851 kb on - strand, within BWI76_RS03230 at 665.874 kb on - strand, within BWI76_RS03230 at 665.875 kb on + strand, within BWI76_RS03230 at 665.878 kb on - strand, within BWI76_RS03230 at 665.904 kb on + strand, within BWI76_RS03230 at 665.912 kb on - strand, within BWI76_RS03230 at 665.932 kb on + strand, within BWI76_RS03230 at 665.943 kb on - strand, within BWI76_RS03230 at 666.032 kb on + strand, within BWI76_RS03230 at 666.040 kb on - strand, within BWI76_RS03230 at 666.087 kb on - strand, within BWI76_RS03230 at 666.136 kb on + strand, within BWI76_RS03230 at 666.161 kb on + strand, within BWI76_RS03230 at 666.201 kb on - strand, within BWI76_RS03230 at 666.214 kb on - strand, within BWI76_RS03230 at 666.237 kb on + strand, within BWI76_RS03230 at 666.238 kb on - strand, within BWI76_RS03230 at 666.303 kb on + strand, within BWI76_RS03230 at 666.303 kb on + strand, within BWI76_RS03230 at 666.316 kb on + strand, within BWI76_RS03230 at 666.319 kb on - strand, within BWI76_RS03230 at 666.326 kb on - strand, within BWI76_RS03230 at 666.326 kb on - strand, within BWI76_RS03230 at 666.330 kb on + strand, within BWI76_RS03230 at 666.330 kb on + strand, within BWI76_RS03230 at 666.330 kb on + strand, within BWI76_RS03230 at 666.330 kb on + strand, within BWI76_RS03230 at 666.333 kb on - strand, within BWI76_RS03230 at 666.335 kb on + strand, within BWI76_RS03230 at 666.335 kb on + strand, within BWI76_RS03230 at 666.335 kb on + strand, within BWI76_RS03230 at 666.335 kb on + strand, within BWI76_RS03230 at 666.338 kb on - strand, within BWI76_RS03230 at 666.338 kb on - strand, within BWI76_RS03230 at 666.338 kb on - strand, within BWI76_RS03230 at 666.338 kb on - strand, within BWI76_RS03230 at 666.338 kb on - strand, within BWI76_RS03230 at 666.343 kb on - strand, within BWI76_RS03230 at 666.343 kb on - strand, within BWI76_RS03230 at 666.343 kb on - strand, within BWI76_RS03230 at 666.343 kb on - strand, within BWI76_RS03230 at 666.406 kb on + strand, within BWI76_RS03230 at 666.415 kb on - strand, within BWI76_RS03230 at 666.415 kb on - strand, within BWI76_RS03230 at 666.415 kb on - strand, within BWI76_RS03230 at 666.416 kb on - strand, within BWI76_RS03230 at 666.436 kb on - strand at 666.442 kb on - strand at 666.451 kb on - strand at 666.451 kb on - strand at 666.518 kb on - strand at 666.528 kb on + strand at 666.549 kb on - strand at 666.564 kb on - strand at 666.570 kb on - strand at 666.571 kb on + strand at 666.579 kb on - strand at 666.583 kb on + strand at 666.585 kb on + strand at 666.585 kb on + strand at 666.591 kb on - strand at 666.593 kb on - strand at 666.593 kb on - strand at 666.593 kb on - strand at 666.593 kb on - strand at 666.638 kb on + strand at 666.654 kb on - strand at 666.764 kb on - strand, within BWI76_RS03235 at 666.775 kb on + strand, within BWI76_RS03235 at 666.811 kb on + strand, within BWI76_RS03235 at 666.820 kb on + strand, within BWI76_RS03235 at 666.828 kb on - strand, within BWI76_RS03235 at 666.838 kb on - strand, within BWI76_RS03235 at 666.844 kb on - strand, within BWI76_RS03235 at 666.870 kb on + strand, within BWI76_RS03235 at 666.896 kb on - strand, within BWI76_RS03235 at 666.896 kb on - strand, within BWI76_RS03235 at 666.904 kb on - strand, within BWI76_RS03235 at 666.911 kb on + strand, within BWI76_RS03235 at 666.919 kb on - strand, within BWI76_RS03235 at 666.920 kb on - strand, within BWI76_RS03235 at 666.935 kb on - strand, within BWI76_RS03235 at 666.945 kb on - strand, within BWI76_RS03235 at 666.945 kb on - strand, within BWI76_RS03235 at 666.945 kb on - strand, within BWI76_RS03235 at 666.985 kb on - strand, within BWI76_RS03235 at 666.996 kb on - strand, within BWI76_RS03235 at 667.085 kb on - strand, within BWI76_RS03235 at 667.096 kb on - strand, within BWI76_RS03235 at 667.096 kb on - strand, within BWI76_RS03235 at 667.096 kb on - strand, within BWI76_RS03235 at 667.101 kb on - strand, within BWI76_RS03235 at 667.115 kb on + strand at 667.115 kb on + strand, within BWI76_RS03235 at 667.118 kb on - strand, within BWI76_RS03235 at 667.123 kb on - strand, within BWI76_RS03235 at 667.123 kb on - strand, within BWI76_RS03235 at 667.128 kb on - strand, within BWI76_RS03235 at 667.130 kb on + strand, within BWI76_RS03235 at 667.132 kb on + strand, within BWI76_RS03235 at 667.132 kb on + strand, within BWI76_RS03235 at 667.132 kb on + strand, within BWI76_RS03235 at 667.132 kb on + strand, within BWI76_RS03235 at 667.133 kb on - strand, within BWI76_RS03235 at 667.138 kb on - strand, within BWI76_RS03235 at 667.140 kb on - strand, within BWI76_RS03235 at 667.140 kb on - strand, within BWI76_RS03235 at 667.140 kb on - strand, within BWI76_RS03235 at 667.140 kb on - strand, within BWI76_RS03235 at 667.140 kb on - strand, within BWI76_RS03235 at 667.180 kb on + strand, within BWI76_RS03235 at 667.238 kb on + strand, within BWI76_RS03235 at 667.242 kb on + strand, within BWI76_RS03235 at 667.243 kb on + strand, within BWI76_RS03235 at 667.243 kb on + strand, within BWI76_RS03235 at 667.243 kb on + strand at 667.251 kb on - strand, within BWI76_RS03235 at 667.314 kb on + strand, within BWI76_RS03235 at 667.449 kb on + strand, within BWI76_RS03235 at 667.480 kb on + strand, within BWI76_RS03235 at 667.488 kb on - strand, within BWI76_RS03235 at 667.488 kb on - strand, within BWI76_RS03235 at 667.514 kb on + strand, within BWI76_RS03235
Per-strain Table
Position Strand Gene LocusTag Fraction RCH2_defined_noCarbon with Mixed culture; Ralstonia sp. UNC404CL21Col 3:1 starting OD to Klebsiella and Glu_DLac_Acetate_mix 1 x remove 664,627 - +0.1 664,649 + +1.0 664,649 + +0.4 664,658 + -0.5 664,660 + -0.5 664,660 + +2.5 664,660 + -1.9 664,660 + -0.9 664,660 + +0.9 664,660 + +0.1 664,660 + +0.6 664,660 + -1.0 664,660 + -0.5 664,660 + +1.3 664,666 + -0.8 664,668 + -1.5 664,668 + +0.1 664,668 + -0.5 664,668 - -3.2 664,668 - -1.5 664,668 - -2.2 664,668 - +1.1 664,668 - -3.0 664,668 - +1.1 664,668 - -4.4 664,668 - +0.1 664,668 - +0.9 664,668 - -1.7 664,668 - -0.2 664,668 - +1.1 664,668 - -1.3 664,668 - +1.5 664,668 - -0.9 664,668 - -0.3 664,671 + -1.5 664,679 - -0.5 664,679 - -1.8 664,681 + -0.7 664,690 + -0.5 664,696 - -1.7 664,700 + +0.9 664,719 + +0.9 664,738 + -2.5 664,738 + -0.4 664,748 - -2.4 664,748 - -1.5 664,793 - +0.8 664,795 + -2.7 664,798 - -4.3 664,820 + -0.5 664,836 - -1.4 665,006 - BWI76_RS03225 0.30 -1.3 665,091 + BWI76_RS03225 0.41 +0.1 665,100 + BWI76_RS03225 0.42 -0.9 665,100 + BWI76_RS03225 0.42 +1.6 665,108 - -1.2 665,108 - BWI76_RS03225 0.43 +0.6 665,130 - BWI76_RS03225 0.45 -0.7 665,130 - BWI76_RS03225 0.45 +0.6 665,155 - BWI76_RS03225 0.48 -0.5 665,155 - BWI76_RS03225 0.48 -2.4 665,155 - BWI76_RS03225 0.48 -2.2 665,155 - BWI76_RS03225 0.48 +4.3 665,158 + BWI76_RS03225 0.49 -0.9 665,190 - BWI76_RS03225 0.53 -3.1 665,261 - BWI76_RS03225 0.62 -0.5 665,261 - BWI76_RS03225 0.62 -0.5 665,275 + BWI76_RS03225 0.63 +2.1 665,279 - BWI76_RS03225 0.64 +1.1 665,307 - BWI76_RS03225 0.67 +1.1 665,443 - BWI76_RS03225 0.84 -0.0 665,445 - BWI76_RS03225 0.84 -3.0 665,476 - BWI76_RS03225 0.88 -2.7 665,477 - BWI76_RS03225 0.88 +1.1 665,513 - -2.6 665,513 - -2.9 665,513 - -1.4 665,513 - -1.5 665,513 - -4.7 665,543 - +0.7 665,543 - -2.2 665,543 - +0.2 665,559 - -3.5 665,579 + +0.1 665,580 - +0.1 665,580 - -1.7 665,587 - -1.7 665,630 + -2.9 665,630 + +0.9 665,630 + +3.7 665,630 + +1.3 665,630 + -0.9 665,630 + +0.7 665,630 + +0.2 665,630 + -0.9 665,630 + +1.1 665,630 + -0.2 665,630 + +2.3 665,630 + -2.1 665,630 + +0.2 665,630 + -0.9 665,630 + -1.7 665,631 + -3.2 665,635 + +0.0 665,635 + -2.2 665,638 - -0.5 665,638 - -1.8 665,638 - -1.7 665,638 - -1.5 665,638 - -0.1 665,638 - +0.2 665,638 - -1.9 665,638 - -0.9 665,638 - -1.2 665,638 - -1.9 665,638 - +1.9 665,638 - -1.4 665,638 - +0.2 665,640 - -3.7 665,645 - -0.5 665,648 - -1.7 665,648 - -0.9 665,648 - +0.1 665,662 + -1.6 665,677 - -0.5 665,739 + BWI76_RS03230 0.13 +0.1 665,753 - BWI76_RS03230 0.15 -1.9 665,753 - BWI76_RS03230 0.15 -0.1 665,775 + BWI76_RS03230 0.17 +0.1 665,805 - BWI76_RS03230 0.21 +1.1 665,812 - BWI76_RS03230 0.21 +0.1 665,841 + BWI76_RS03230 0.25 -3.2 665,843 + BWI76_RS03230 0.25 -0.5 665,848 + BWI76_RS03230 0.25 +0.1 665,848 + BWI76_RS03230 0.25 -3.0 665,848 + BWI76_RS03230 0.25 -0.6 665,851 - BWI76_RS03230 0.26 -0.9 665,874 - BWI76_RS03230 0.28 +1.4 665,875 + BWI76_RS03230 0.28 -3.5 665,878 - BWI76_RS03230 0.29 +0.1 665,904 + BWI76_RS03230 0.31 -0.2 665,912 - BWI76_RS03230 0.32 +0.2 665,932 + BWI76_RS03230 0.35 +0.1 665,943 - BWI76_RS03230 0.36 -2.3 666,032 + BWI76_RS03230 0.46 -1.5 666,040 - BWI76_RS03230 0.46 +0.1 666,087 - BWI76_RS03230 0.52 -0.7 666,136 + BWI76_RS03230 0.57 -0.5 666,161 + BWI76_RS03230 0.60 -4.4 666,201 - BWI76_RS03230 0.64 -1.5 666,214 - BWI76_RS03230 0.66 -1.7 666,237 + BWI76_RS03230 0.68 +1.1 666,238 - BWI76_RS03230 0.68 -0.5 666,303 + BWI76_RS03230 0.75 +0.7 666,303 + BWI76_RS03230 0.75 -1.2 666,316 + BWI76_RS03230 0.77 +0.1 666,319 - BWI76_RS03230 0.77 +2.1 666,326 - BWI76_RS03230 0.78 +1.3 666,326 - BWI76_RS03230 0.78 -1.3 666,330 + BWI76_RS03230 0.78 +1.1 666,330 + BWI76_RS03230 0.78 +1.1 666,330 + BWI76_RS03230 0.78 -1.5 666,330 + BWI76_RS03230 0.78 -0.9 666,333 - BWI76_RS03230 0.79 -0.9 666,335 + BWI76_RS03230 0.79 -0.3 666,335 + BWI76_RS03230 0.79 -1.2 666,335 + BWI76_RS03230 0.79 +1.1 666,335 + BWI76_RS03230 0.79 +0.5 666,338 - BWI76_RS03230 0.79 +1.1 666,338 - BWI76_RS03230 0.79 +0.1 666,338 - BWI76_RS03230 0.79 -4.5 666,338 - BWI76_RS03230 0.79 -0.8 666,338 - BWI76_RS03230 0.79 +1.4 666,343 - BWI76_RS03230 0.80 -1.9 666,343 - BWI76_RS03230 0.80 -1.2 666,343 - BWI76_RS03230 0.80 -0.5 666,343 - BWI76_RS03230 0.80 +1.1 666,406 + BWI76_RS03230 0.87 -0.5 666,415 - BWI76_RS03230 0.88 -0.5 666,415 - BWI76_RS03230 0.88 -2.0 666,415 - BWI76_RS03230 0.88 +1.1 666,416 - BWI76_RS03230 0.88 -3.2 666,436 - -1.7 666,442 - +1.1 666,451 - -1.0 666,451 - -0.5 666,518 - +0.1 666,528 + -1.2 666,549 - -0.5 666,564 - -0.3 666,570 - +2.7 666,571 + -0.5 666,579 - -1.5 666,583 + -1.5 666,585 + -0.9 666,585 + -1.2 666,591 - +0.1 666,593 - +1.7 666,593 - -2.4 666,593 - -0.2 666,593 - -4.1 666,638 + -2.8 666,654 - -1.9 666,764 - BWI76_RS03235 0.14 -0.5 666,775 + BWI76_RS03235 0.14 +1.1 666,811 + BWI76_RS03235 0.16 +0.7 666,820 + BWI76_RS03235 0.17 +1.1 666,828 - BWI76_RS03235 0.17 +2.7 666,838 - BWI76_RS03235 0.18 -0.5 666,844 - BWI76_RS03235 0.18 -1.0 666,870 + BWI76_RS03235 0.19 +0.3 666,896 - BWI76_RS03235 0.21 +0.3 666,896 - BWI76_RS03235 0.21 -0.4 666,904 - BWI76_RS03235 0.21 -0.2 666,911 + BWI76_RS03235 0.22 -0.5 666,919 - BWI76_RS03235 0.22 -1.3 666,920 - BWI76_RS03235 0.22 -1.5 666,935 - BWI76_RS03235 0.23 -0.9 666,945 - BWI76_RS03235 0.24 +0.1 666,945 - BWI76_RS03235 0.24 -0.4 666,945 - BWI76_RS03235 0.24 +0.1 666,985 - BWI76_RS03235 0.26 +0.1 666,996 - BWI76_RS03235 0.26 -0.2 667,085 - BWI76_RS03235 0.31 -0.5 667,096 - BWI76_RS03235 0.32 +1.0 667,096 - BWI76_RS03235 0.32 -1.7 667,096 - BWI76_RS03235 0.32 +0.8 667,101 - BWI76_RS03235 0.32 -0.4 667,115 + +1.1 667,115 + BWI76_RS03235 0.33 -2.6 667,118 - BWI76_RS03235 0.33 +0.1 667,123 - BWI76_RS03235 0.34 -0.2 667,123 - BWI76_RS03235 0.34 -3.1 667,128 - BWI76_RS03235 0.34 +0.1 667,130 + BWI76_RS03235 0.34 +1.1 667,132 + BWI76_RS03235 0.34 +0.1 667,132 + BWI76_RS03235 0.34 -1.2 667,132 + BWI76_RS03235 0.34 -0.5 667,132 + BWI76_RS03235 0.34 +1.1 667,133 - BWI76_RS03235 0.34 +2.4 667,138 - BWI76_RS03235 0.34 -3.0 667,140 - BWI76_RS03235 0.34 -1.9 667,140 - BWI76_RS03235 0.34 +0.1 667,140 - BWI76_RS03235 0.34 -0.5 667,140 - BWI76_RS03235 0.34 +1.5 667,140 - BWI76_RS03235 0.34 +0.1 667,180 + BWI76_RS03235 0.37 -2.7 667,238 + BWI76_RS03235 0.40 -0.5 667,242 + BWI76_RS03235 0.40 -0.5 667,243 + BWI76_RS03235 0.40 +0.1 667,243 + BWI76_RS03235 0.40 -2.1 667,243 + +0.1 667,251 - BWI76_RS03235 0.41 -0.2 667,314 + BWI76_RS03235 0.44 +1.1 667,449 + BWI76_RS03235 0.52 +1.2 667,480 + BWI76_RS03235 0.53 +1.1 667,488 - BWI76_RS03235 0.54 -1.2 667,488 - BWI76_RS03235 0.54 -2.4 667,514 + BWI76_RS03235 0.55 +0.1
Or see this region's nucleotide sequence