Experiment: RCH2_defined_noCarbon with Mixed culture; Ralstonia sp. UNC404CL21Col 3:1 starting OD to Klebsiella and Glu_DLac_Acetate_mix 1 x
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS03070 and BWI76_RS03075 are separated by 18 nucleotides BWI76_RS03075 and BWI76_RS03080 are separated by 196 nucleotides
BWI76_RS03070: BWI76_RS03070 - Methylmalonate-semialdehyde dehydrogenase [inositol] (EC 1.2.1.27) <small>(from data)</small>, at 629,200 to 630,705
_RS03070
BWI76_RS03075: BWI76_RS03075 - 5-deoxy-D-glucuronate isomerase (EC 5.3.1.30) <small>(from data)</small>, at 630,724 to 631,533
_RS03075
BWI76_RS03080: BWI76_RS03080 - (Fe-S)-cluster assembly protein, at 631,730 to 632,587
_RS03080
Position (kb) 630
631
632 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1
2 at 629.832 kb on + strand, within BWI76_RS03070 at 629.837 kb on + strand, within BWI76_RS03070 at 629.840 kb on - strand, within BWI76_RS03070 at 629.845 kb on - strand, within BWI76_RS03070 at 629.947 kb on + strand, within BWI76_RS03070 at 630.110 kb on - strand, within BWI76_RS03070 at 630.152 kb on + strand, within BWI76_RS03070 at 630.239 kb on + strand, within BWI76_RS03070 at 630.394 kb on + strand, within BWI76_RS03070 at 630.516 kb on + strand, within BWI76_RS03070 at 630.517 kb on - strand, within BWI76_RS03070 at 630.819 kb on - strand, within BWI76_RS03075 at 630.869 kb on + strand, within BWI76_RS03075 at 630.899 kb on - strand, within BWI76_RS03075 at 630.926 kb on + strand, within BWI76_RS03075 at 630.990 kb on + strand, within BWI76_RS03075 at 630.990 kb on + strand, within BWI76_RS03075 at 630.998 kb on - strand, within BWI76_RS03075 at 631.193 kb on - strand, within BWI76_RS03075 at 631.350 kb on + strand, within BWI76_RS03075 at 631.367 kb on + strand, within BWI76_RS03075 at 631.471 kb on + strand at 631.508 kb on + strand at 631.639 kb on - strand at 631.711 kb on + strand at 631.852 kb on + strand, within BWI76_RS03080 at 631.860 kb on - strand, within BWI76_RS03080 at 631.932 kb on + strand, within BWI76_RS03080 at 632.081 kb on - strand, within BWI76_RS03080 at 632.086 kb on - strand, within BWI76_RS03080 at 632.231 kb on - strand, within BWI76_RS03080
Per-strain Table
Position Strand Gene LocusTag Fraction RCH2_defined_noCarbon with Mixed culture; Ralstonia sp. UNC404CL21Col 3:1 starting OD to Klebsiella and Glu_DLac_Acetate_mix 1 x remove 629,832 + BWI76_RS03070 0.42 +1.1 629,837 + BWI76_RS03070 0.42 +1.2 629,840 - BWI76_RS03070 0.42 -0.9 629,845 - BWI76_RS03070 0.43 -3.1 629,947 + BWI76_RS03070 0.50 +1.4 630,110 - BWI76_RS03070 0.60 -1.4 630,152 + BWI76_RS03070 0.63 -0.4 630,239 + BWI76_RS03070 0.69 +0.0 630,394 + BWI76_RS03070 0.79 -2.6 630,516 + BWI76_RS03070 0.87 +1.1 630,517 - BWI76_RS03070 0.87 +0.2 630,819 - BWI76_RS03075 0.12 +0.1 630,869 + BWI76_RS03075 0.18 -2.9 630,899 - BWI76_RS03075 0.22 -1.5 630,926 + BWI76_RS03075 0.25 +1.1 630,990 + BWI76_RS03075 0.33 +0.3 630,990 + BWI76_RS03075 0.33 +0.1 630,998 - BWI76_RS03075 0.34 -0.2 631,193 - BWI76_RS03075 0.58 +0.7 631,350 + BWI76_RS03075 0.77 +2.7 631,367 + BWI76_RS03075 0.79 +0.1 631,471 + +2.1 631,508 + -0.3 631,639 - +0.4 631,711 + +1.3 631,852 + BWI76_RS03080 0.14 -0.9 631,860 - BWI76_RS03080 0.15 -3.9 631,932 + BWI76_RS03080 0.24 -1.4 632,081 - BWI76_RS03080 0.41 -4.6 632,086 - BWI76_RS03080 0.41 -1.7 632,231 - BWI76_RS03080 0.58 +1.1
Or see this region's nucleotide sequence