Experiment: RCH2_defined_noCarbon with Mixed culture; Ralstonia sp. UNC404CL21Col 3:1 starting OD to Klebsiella and Glu_DLac_Acetate_mix 1 x
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS01700 and BWI76_RS01705 are separated by 236 nucleotides BWI76_RS01705 and BWI76_RS01710 overlap by 1 nucleotides BWI76_RS01710 and BWI76_RS01715 are separated by 31 nucleotides BWI76_RS01715 and BWI76_RS01720 overlap by 8 nucleotides
BWI76_RS01700: BWI76_RS01700 - dipeptidase E, at 350,137 to 350,826
_RS01700
BWI76_RS01705: BWI76_RS01705 - CcdB antidote CcdA, at 351,063 to 351,356
_RS01705
BWI76_RS01710: BWI76_RS01710 - DNA gyrase inhibitor, at 351,356 to 351,673
_RS01710
BWI76_RS01715: BWI76_RS01715 - shikimate 5-dehydrogenase, at 351,705 to 352,514
_RS01715
BWI76_RS01720: BWI76_RS01720 - L-sorbose 1-phosphate reductase (EC 1.1.1.-) <small>(from data)</small>, at 352,507 to 353,736
_RS01720
Position (kb) 351
352
353 Strain fitness (log2 ratio) -2
-1
0
1
2
3 at 350.722 kb on - strand, within BWI76_RS01700 at 350.780 kb on - strand at 350.908 kb on - strand at 350.909 kb on + strand at 350.922 kb on - strand at 350.922 kb on - strand at 350.963 kb on - strand at 351.034 kb on - strand at 351.258 kb on + strand, within BWI76_RS01705 at 351.310 kb on + strand, within BWI76_RS01705 at 351.443 kb on + strand, within BWI76_RS01710 at 351.458 kb on - strand, within BWI76_RS01710 at 351.622 kb on + strand, within BWI76_RS01710 at 351.622 kb on + strand, within BWI76_RS01710 at 351.627 kb on + strand, within BWI76_RS01710 at 351.627 kb on + strand, within BWI76_RS01710 at 351.627 kb on + strand, within BWI76_RS01710 at 351.630 kb on - strand, within BWI76_RS01710 at 351.630 kb on - strand, within BWI76_RS01710 at 351.630 kb on - strand, within BWI76_RS01710 at 351.635 kb on - strand, within BWI76_RS01710 at 351.682 kb on + strand at 351.682 kb on + strand at 351.690 kb on - strand at 351.722 kb on - strand at 351.777 kb on + strand at 351.834 kb on + strand, within BWI76_RS01715 at 351.988 kb on - strand, within BWI76_RS01715 at 351.999 kb on - strand, within BWI76_RS01715 at 352.145 kb on - strand, within BWI76_RS01715 at 352.218 kb on - strand, within BWI76_RS01715 at 352.249 kb on + strand, within BWI76_RS01715 at 352.280 kb on + strand, within BWI76_RS01715 at 352.309 kb on - strand, within BWI76_RS01715 at 352.346 kb on - strand, within BWI76_RS01715 at 352.391 kb on - strand, within BWI76_RS01715 at 352.392 kb on + strand, within BWI76_RS01715 at 352.416 kb on - strand, within BWI76_RS01715 at 352.486 kb on + strand at 352.687 kb on + strand, within BWI76_RS01720 at 352.722 kb on + strand, within BWI76_RS01720 at 352.724 kb on - strand, within BWI76_RS01720 at 352.930 kb on + strand, within BWI76_RS01720 at 353.173 kb on + strand, within BWI76_RS01720 at 353.189 kb on + strand, within BWI76_RS01720 at 353.450 kb on - strand, within BWI76_RS01720 at 353.467 kb on + strand, within BWI76_RS01720 at 353.467 kb on + strand, within BWI76_RS01720
Per-strain Table
Position Strand Gene LocusTag Fraction RCH2_defined_noCarbon with Mixed culture; Ralstonia sp. UNC404CL21Col 3:1 starting OD to Klebsiella and Glu_DLac_Acetate_mix 1 x remove 350,722 - BWI76_RS01700 0.85 -1.6 350,780 - +1.9 350,908 - +0.5 350,909 + +0.7 350,922 - -0.0 350,922 - +0.8 350,963 - +0.8 351,034 - +0.7 351,258 + BWI76_RS01705 0.66 -1.4 351,310 + BWI76_RS01705 0.84 +1.1 351,443 + BWI76_RS01710 0.27 +0.3 351,458 - BWI76_RS01710 0.32 -0.4 351,622 + BWI76_RS01710 0.84 +0.7 351,622 + BWI76_RS01710 0.84 +1.5 351,627 + BWI76_RS01710 0.85 +0.4 351,627 + BWI76_RS01710 0.85 +0.7 351,627 + BWI76_RS01710 0.85 +0.4 351,630 - BWI76_RS01710 0.86 -1.2 351,630 - BWI76_RS01710 0.86 +0.2 351,630 - BWI76_RS01710 0.86 -1.6 351,635 - BWI76_RS01710 0.88 +0.5 351,682 + +3.0 351,682 + -0.2 351,690 - +0.4 351,722 - -0.2 351,777 + -0.1 351,834 + BWI76_RS01715 0.16 -0.8 351,988 - BWI76_RS01715 0.35 +0.5 351,999 - BWI76_RS01715 0.36 +1.1 352,145 - BWI76_RS01715 0.54 +0.7 352,218 - BWI76_RS01715 0.63 +1.0 352,249 + BWI76_RS01715 0.67 +2.2 352,280 + BWI76_RS01715 0.71 +1.1 352,309 - BWI76_RS01715 0.75 +1.7 352,346 - BWI76_RS01715 0.79 +1.4 352,391 - BWI76_RS01715 0.85 +1.2 352,392 + BWI76_RS01715 0.85 +0.8 352,416 - BWI76_RS01715 0.88 +1.9 352,486 + +0.4 352,687 + BWI76_RS01720 0.15 +0.9 352,722 + BWI76_RS01720 0.17 +1.4 352,724 - BWI76_RS01720 0.18 -2.0 352,930 + BWI76_RS01720 0.34 +1.7 353,173 + BWI76_RS01720 0.54 +0.1 353,189 + BWI76_RS01720 0.55 +0.2 353,450 - BWI76_RS01720 0.77 +0.5 353,467 + BWI76_RS01720 0.78 +1.4 353,467 + BWI76_RS01720 0.78 -0.4
Or see this region's nucleotide sequence