Experiment: RCH2_defined_noCarbon with Mixed culture; Ralstonia sp. UNC404CL21Col 3:1 starting OD to Klebsiella and Glu_DLac_Acetate_mix 1 x
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS00735 and BWI76_RS00740 are separated by 60 nucleotides BWI76_RS00740 and BWI76_RS00745 are separated by 92 nucleotides BWI76_RS00745 and BWI76_RS00750 are separated by 244 nucleotides
BWI76_RS00735: BWI76_RS00735 - ATP-dependent 6-phosphofructokinase, at 149,499 to 150,461
_RS00735
BWI76_RS00740: BWI76_RS00740 - ribonuclease E activity regulator RraA, at 150,522 to 151,007
_RS00740
BWI76_RS00745: BWI76_RS00745 - 1,4-dihydroxy-2-naphthoate polyprenyltransferase, at 151,100 to 152,026
_RS00745
BWI76_RS00750: BWI76_RS00750 - ATP-dependent protease ATP-binding subunit HslU, at 152,271 to 153,605
_RS00750
Position (kb) 151
152
153 Strain fitness (log2 ratio) -3
-2
-1
0
1 at 150.559 kb on - strand at 150.683 kb on - strand, within BWI76_RS00740 at 150.827 kb on + strand, within BWI76_RS00740 at 150.827 kb on - strand, within BWI76_RS00740 at 150.867 kb on - strand, within BWI76_RS00740 at 150.867 kb on - strand, within BWI76_RS00740 at 150.963 kb on - strand at 151.169 kb on - strand at 151.176 kb on - strand at 151.316 kb on - strand, within BWI76_RS00745 at 151.316 kb on - strand, within BWI76_RS00745 at 151.443 kb on + strand, within BWI76_RS00745 at 151.489 kb on + strand, within BWI76_RS00745 at 151.489 kb on + strand, within BWI76_RS00745 at 151.497 kb on - strand, within BWI76_RS00745 at 151.497 kb on - strand, within BWI76_RS00745 at 152.095 kb on + strand at 152.172 kb on + strand at 152.206 kb on - strand at 152.239 kb on + strand at 152.239 kb on + strand at 152.243 kb on - strand at 152.247 kb on - strand at 152.481 kb on + strand, within BWI76_RS00750 at 152.533 kb on + strand, within BWI76_RS00750 at 152.745 kb on - strand, within BWI76_RS00750 at 152.784 kb on - strand, within BWI76_RS00750 at 152.931 kb on - strand, within BWI76_RS00750
Per-strain Table
Position Strand Gene LocusTag Fraction RCH2_defined_noCarbon with Mixed culture; Ralstonia sp. UNC404CL21Col 3:1 starting OD to Klebsiella and Glu_DLac_Acetate_mix 1 x remove 150,559 - +0.3 150,683 - BWI76_RS00740 0.33 +0.5 150,827 + BWI76_RS00740 0.63 +0.3 150,827 - BWI76_RS00740 0.63 +0.8 150,867 - BWI76_RS00740 0.71 -0.4 150,867 - BWI76_RS00740 0.71 +0.9 150,963 - +0.7 151,169 - -0.8 151,176 - +1.3 151,316 - BWI76_RS00745 0.23 +0.3 151,316 - BWI76_RS00745 0.23 -0.1 151,443 + BWI76_RS00745 0.37 -0.7 151,489 + BWI76_RS00745 0.42 -1.0 151,489 + BWI76_RS00745 0.42 -1.4 151,497 - BWI76_RS00745 0.43 +0.3 151,497 - BWI76_RS00745 0.43 -3.7 152,095 + +0.4 152,172 + -1.6 152,206 - +0.3 152,239 + -0.5 152,239 + -1.2 152,243 - +1.5 152,247 - -0.6 152,481 + BWI76_RS00750 0.16 -2.7 152,533 + BWI76_RS00750 0.20 -0.2 152,745 - BWI76_RS00750 0.36 -0.9 152,784 - BWI76_RS00750 0.38 -0.4 152,931 - BWI76_RS00750 0.49 +1.5
Or see this region's nucleotide sequence