Experiment: Vogels_fungal_media 0.1X
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BPHYT_RS20620 and BPHYT_RS20625 are separated by 60 nucleotides BPHYT_RS20625 and BPHYT_RS20630 are separated by 218 nucleotides BPHYT_RS20630 and BPHYT_RS20635 are separated by 625 nucleotides
BPHYT_RS20620: BPHYT_RS20620 - 4-oxalocrotonate tautomerase, at 185,095 to 185,331
_RS20620
BPHYT_RS20625: BPHYT_RS20625 - 3-oxoacyl-ACP reductase, at 185,392 to 186,102
_RS20625
BPHYT_RS20630: BPHYT_RS20630 - patatin, at 186,321 to 187,484
_RS20630
BPHYT_RS20635: BPHYT_RS20635 - RND transporter, at 188,110 to 189,792
_RS20635
Position (kb) 186
187
188 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 185.325 kb on + strand at 185.609 kb on + strand, within BPHYT_RS20625 at 185.829 kb on - strand, within BPHYT_RS20625 at 185.829 kb on - strand, within BPHYT_RS20625 at 186.118 kb on + strand at 186.123 kb on - strand at 186.123 kb on - strand at 186.165 kb on + strand at 186.322 kb on + strand at 186.938 kb on - strand, within BPHYT_RS20630 at 187.634 kb on + strand at 187.785 kb on + strand at 187.804 kb on + strand at 187.804 kb on + strand at 187.805 kb on - strand at 187.983 kb on + strand at 188.147 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Vogels_fungal_media 0.1X remove 185,325 + -1.0 185,609 + BPHYT_RS20625 0.31 +1.5 185,829 - BPHYT_RS20625 0.61 -0.1 185,829 - BPHYT_RS20625 0.61 -1.0 186,118 + -2.2 186,123 - +0.0 186,123 - -3.0 186,165 + -0.3 186,322 + +1.0 186,938 - BPHYT_RS20630 0.53 -1.5 187,634 + -0.4 187,785 + +0.3 187,804 + -0.9 187,804 + +2.4 187,805 - -0.0 187,983 + +0.3 188,147 + +0.5
Or see this region's nucleotide sequence