Experiment: Vogels_fungal_media 0.1X
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BPHYT_RS10065 and BPHYT_RS10070 are separated by 44 nucleotides BPHYT_RS10070 and BPHYT_RS10075 are separated by 98 nucleotides BPHYT_RS10075 and BPHYT_RS10080 are separated by 62 nucleotides BPHYT_RS10080 and BPHYT_RS10085 are separated by 54 nucleotides
BPHYT_RS10065: BPHYT_RS10065 - cytochrome C oxidase subunit I, at 2,292,662 to 2,293,306
_RS10065
BPHYT_RS10070: BPHYT_RS10070 - hypothetical protein, at 2,293,351 to 2,293,734
_RS10070
BPHYT_RS10075: BPHYT_RS10075 - alcohol dehydrogenase, at 2,293,833 to 2,295,134
_RS10075
BPHYT_RS10080: BPHYT_RS10080 - hypothetical protein, at 2,295,197 to 2,295,415
_RS10080
BPHYT_RS10085: BPHYT_RS10085 - thiamine pyrophosphate protein, at 2,295,470 to 2,297,311
_RS10085
Position (kb)
2293
2294
2295
2296 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1 at 2293.005 kb on + strand, within BPHYT_RS10065 at 2293.080 kb on - strand, within BPHYT_RS10065 at 2293.102 kb on + strand, within BPHYT_RS10065 at 2293.514 kb on + strand, within BPHYT_RS10070 at 2293.515 kb on - strand, within BPHYT_RS10070 at 2293.749 kb on - strand at 2294.668 kb on - strand, within BPHYT_RS10075 at 2294.703 kb on + strand, within BPHYT_RS10075 at 2295.132 kb on + strand at 2295.156 kb on + strand at 2295.471 kb on + strand at 2295.472 kb on - strand at 2295.598 kb on + strand at 2295.598 kb on + strand at 2295.711 kb on + strand, within BPHYT_RS10085 at 2295.712 kb on - strand, within BPHYT_RS10085 at 2295.767 kb on - strand, within BPHYT_RS10085
Per-strain Table
Position Strand Gene LocusTag Fraction Vogels_fungal_media 0.1X remove 2,293,005 + BPHYT_RS10065 0.53 -0.4 2,293,080 - BPHYT_RS10065 0.65 +0.3 2,293,102 + BPHYT_RS10065 0.68 -0.4 2,293,514 + BPHYT_RS10070 0.42 -4.6 2,293,515 - BPHYT_RS10070 0.43 +0.4 2,293,749 - +1.1 2,294,668 - BPHYT_RS10075 0.64 -1.8 2,294,703 + BPHYT_RS10075 0.67 +0.0 2,295,132 + +0.2 2,295,156 + -1.0 2,295,471 + -0.0 2,295,472 - +0.2 2,295,598 + +0.2 2,295,598 + -0.2 2,295,711 + BPHYT_RS10085 0.13 +1.5 2,295,712 - BPHYT_RS10085 0.13 +0.2 2,295,767 - BPHYT_RS10085 0.16 +1.4
Or see this region's nucleotide sequence