Strain Fitness in Burkholderia phytofirmans PsJN around BPHYT_RS27240

Experiment: Vogels_fungal_media 0.2X; growth supplemented with 0.4X LB

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntBPHYT_RS27230 and BPHYT_RS27235 are separated by 186 nucleotidesBPHYT_RS27235 and BPHYT_RS27240 are separated by 142 nucleotidesBPHYT_RS27240 and BPHYT_RS27245 are separated by 330 nucleotidesBPHYT_RS27245 and BPHYT_RS27250 are separated by 154 nucleotides BPHYT_RS27230: BPHYT_RS27230 - hypothetical protein, at 1,686,022 to 1,686,363 _RS27230 BPHYT_RS27235: BPHYT_RS27235 - hypothetical protein, at 1,686,550 to 1,686,906 _RS27235 BPHYT_RS27240: BPHYT_RS27240 - N-ethylmaleimide reductase, at 1,687,049 to 1,688,152 _RS27240 BPHYT_RS27245: BPHYT_RS27245 - hypothetical protein, at 1,688,483 to 1,688,725 _RS27245 BPHYT_RS27250: BPHYT_RS27250 - NAD-dependent epimerase/dehydratase, at 1,688,880 to 1,689,518 _RS27250 Position (kb) 1687 1688 1689Strain fitness (log2 ratio) -2 -1 0 1 2at 1686.076 kb on + strand, within BPHYT_RS27230at 1686.362 kb on - strandat 1687.360 kb on - strand, within BPHYT_RS27240at 1687.360 kb on - strand, within BPHYT_RS27240at 1687.953 kb on + strand, within BPHYT_RS27240at 1688.115 kb on + strandat 1688.115 kb on + strandat 1688.315 kb on - strandat 1688.315 kb on - strandat 1688.356 kb on + strandat 1688.357 kb on - strandat 1688.424 kb on + strandat 1688.428 kb on + strandat 1689.145 kb on + strand, within BPHYT_RS27250

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Per-strain Table

Position Strand Gene LocusTag Fraction Vogels_fungal_media 0.2X; growth supplemented with 0.4X LB
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1,686,076 + BPHYT_RS27230 0.16 +0.7
1,686,362 - +1.1
1,687,360 - BPHYT_RS27240 0.28 +0.7
1,687,360 - BPHYT_RS27240 0.28 +0.7
1,687,953 + BPHYT_RS27240 0.82 +0.7
1,688,115 + +0.3
1,688,115 + +2.0
1,688,315 - +1.0
1,688,315 - +0.4
1,688,356 + +0.9
1,688,357 - -1.3
1,688,424 + +1.9
1,688,428 + -2.2
1,689,145 + BPHYT_RS27250 0.41 -0.6

Or see this region's nucleotide sequence