Experiment: Vogels_fungal_media 0.2X; growth supplemented with 0.4X LB
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BPHYT_RS27230 and BPHYT_RS27235 are separated by 186 nucleotides BPHYT_RS27235 and BPHYT_RS27240 are separated by 142 nucleotides BPHYT_RS27240 and BPHYT_RS27245 are separated by 330 nucleotides BPHYT_RS27245 and BPHYT_RS27250 are separated by 154 nucleotides
BPHYT_RS27230: BPHYT_RS27230 - hypothetical protein, at 1,686,022 to 1,686,363
_RS27230
BPHYT_RS27235: BPHYT_RS27235 - hypothetical protein, at 1,686,550 to 1,686,906
_RS27235
BPHYT_RS27240: BPHYT_RS27240 - N-ethylmaleimide reductase, at 1,687,049 to 1,688,152
_RS27240
BPHYT_RS27245: BPHYT_RS27245 - hypothetical protein, at 1,688,483 to 1,688,725
_RS27245
BPHYT_RS27250: BPHYT_RS27250 - NAD-dependent epimerase/dehydratase, at 1,688,880 to 1,689,518
_RS27250
Position (kb)
1687
1688
1689 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 1686.076 kb on + strand, within BPHYT_RS27230 at 1686.362 kb on - strand at 1687.360 kb on - strand, within BPHYT_RS27240 at 1687.360 kb on - strand, within BPHYT_RS27240 at 1687.953 kb on + strand, within BPHYT_RS27240 at 1688.115 kb on + strand at 1688.115 kb on + strand at 1688.315 kb on - strand at 1688.315 kb on - strand at 1688.356 kb on + strand at 1688.357 kb on - strand at 1688.424 kb on + strand at 1688.428 kb on + strand at 1689.145 kb on + strand, within BPHYT_RS27250
Per-strain Table
Position Strand Gene LocusTag Fraction Vogels_fungal_media 0.2X; growth supplemented with 0.4X LB remove 1,686,076 + BPHYT_RS27230 0.16 +0.7 1,686,362 - +1.1 1,687,360 - BPHYT_RS27240 0.28 +0.7 1,687,360 - BPHYT_RS27240 0.28 +0.7 1,687,953 + BPHYT_RS27240 0.82 +0.7 1,688,115 + +0.3 1,688,115 + +2.0 1,688,315 - +1.0 1,688,315 - +0.4 1,688,356 + +0.9 1,688,357 - -1.3 1,688,424 + +1.9 1,688,428 + -2.2 1,689,145 + BPHYT_RS27250 0.41 -0.6
Or see this region's nucleotide sequence