Experiment: Vogels_fungal_media 0.2X; growth supplemented with 0.4X LB
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BPHYT_RS23635 and BPHYT_RS23640 are separated by 86 nucleotides BPHYT_RS23640 and BPHYT_RS23645 are separated by 113 nucleotides BPHYT_RS23645 and BPHYT_RS23650 are separated by 160 nucleotides
BPHYT_RS23635: BPHYT_RS23635 - phosphoesterase, at 872,681 to 874,687
_RS23635
BPHYT_RS23640: BPHYT_RS23640 - hypothetical protein, at 874,774 to 875,472
_RS23640
BPHYT_RS23645: BPHYT_RS23645 - phosphohydrolase, at 875,586 to 876,230
_RS23645
BPHYT_RS23650: BPHYT_RS23650 - hypothetical protein, at 876,391 to 876,729
_RS23650
Position (kb) 874
875
876 Strain fitness (log2 ratio) -1
0
1 at 873.853 kb on - strand, within BPHYT_RS23635 at 874.437 kb on + strand, within BPHYT_RS23635 at 874.438 kb on - strand, within BPHYT_RS23635 at 874.438 kb on - strand, within BPHYT_RS23635 at 874.665 kb on - strand at 874.665 kb on - strand at 874.674 kb on + strand at 874.726 kb on - strand at 874.860 kb on - strand, within BPHYT_RS23640 at 874.892 kb on + strand, within BPHYT_RS23640 at 874.893 kb on - strand, within BPHYT_RS23640 at 875.073 kb on + strand, within BPHYT_RS23640 at 875.074 kb on - strand, within BPHYT_RS23640 at 875.138 kb on + strand, within BPHYT_RS23640 at 875.501 kb on + strand at 875.560 kb on - strand at 875.770 kb on + strand, within BPHYT_RS23645 at 875.770 kb on + strand, within BPHYT_RS23645 at 875.771 kb on - strand, within BPHYT_RS23645 at 876.037 kb on - strand, within BPHYT_RS23645 at 876.170 kb on - strand at 876.260 kb on + strand at 876.324 kb on + strand at 876.325 kb on - strand at 876.327 kb on + strand at 876.412 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Vogels_fungal_media 0.2X; growth supplemented with 0.4X LB remove 873,853 - BPHYT_RS23635 0.58 -0.3 874,437 + BPHYT_RS23635 0.87 +0.4 874,438 - BPHYT_RS23635 0.88 +1.2 874,438 - BPHYT_RS23635 0.88 -0.1 874,665 - -1.0 874,665 - +0.0 874,674 + -0.1 874,726 - -1.0 874,860 - BPHYT_RS23640 0.12 -0.2 874,892 + BPHYT_RS23640 0.17 -0.1 874,893 - BPHYT_RS23640 0.17 -0.3 875,073 + BPHYT_RS23640 0.43 -0.8 875,074 - BPHYT_RS23640 0.43 -0.0 875,138 + BPHYT_RS23640 0.52 +0.3 875,501 + -1.4 875,560 - +1.4 875,770 + BPHYT_RS23645 0.29 +1.5 875,770 + BPHYT_RS23645 0.29 -0.0 875,771 - BPHYT_RS23645 0.29 +0.5 876,037 - BPHYT_RS23645 0.70 +0.4 876,170 - +0.3 876,260 + -1.1 876,324 + -1.7 876,325 - -1.2 876,327 + -0.2 876,412 - +0.1
Or see this region's nucleotide sequence