Strain Fitness in Burkholderia phytofirmans PsJN around BPHYT_RS14175

Experiment: Vogels_fungal_media 0.2X; growth supplemented with 0.4X LB

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntBPHYT_RS14170 and BPHYT_RS14175 are separated by 183 nucleotidesBPHYT_RS14175 and BPHYT_RS14180 are separated by 145 nucleotidesBPHYT_RS14180 and BPHYT_RS14185 are separated by 20 nucleotides BPHYT_RS14170: BPHYT_RS14170 - ribosomal RNA large subunit methyltransferase E, at 3,237,018 to 3,237,680 _RS14170 BPHYT_RS14175: BPHYT_RS14175 - RNA-binding protein, at 3,237,864 to 3,238,391 _RS14175 BPHYT_RS14180: BPHYT_RS14180 - membrane protein, at 3,238,537 to 3,239,046 _RS14180 BPHYT_RS14185: BPHYT_RS14185 - transcription elongation factor GreA, at 3,239,067 to 3,239,543 _RS14185 Position (kb) 3237 3238 3239Strain fitness (log2 ratio) -2 -1 0 1 2 3at 3236.892 kb on - strandat 3237.020 kb on - strandat 3237.717 kb on - strandat 3237.724 kb on - strandat 3238.059 kb on + strand, within BPHYT_RS14175at 3238.060 kb on - strand, within BPHYT_RS14175at 3238.060 kb on - strand, within BPHYT_RS14175at 3238.103 kb on + strand, within BPHYT_RS14175at 3238.104 kb on - strand, within BPHYT_RS14175at 3238.390 kb on - strandat 3238.502 kb on + strandat 3238.503 kb on - strandat 3238.649 kb on + strand, within BPHYT_RS14180at 3238.695 kb on - strand, within BPHYT_RS14180at 3238.753 kb on - strand, within BPHYT_RS14180at 3238.983 kb on - strand, within BPHYT_RS14180at 3239.074 kb on + strandat 3239.074 kb on + strandat 3239.075 kb on - strandat 3239.095 kb on + strandat 3239.231 kb on - strand, within BPHYT_RS14185

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Per-strain Table

Position Strand Gene LocusTag Fraction Vogels_fungal_media 0.2X; growth supplemented with 0.4X LB
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3,236,892 - -1.9
3,237,020 - +0.1
3,237,717 - -0.4
3,237,724 - -0.1
3,238,059 + BPHYT_RS14175 0.37 -1.0
3,238,060 - BPHYT_RS14175 0.37 -2.1
3,238,060 - BPHYT_RS14175 0.37 -1.2
3,238,103 + BPHYT_RS14175 0.45 -1.0
3,238,104 - BPHYT_RS14175 0.45 -1.0
3,238,390 - +0.0
3,238,502 + -0.2
3,238,503 - -1.1
3,238,649 + BPHYT_RS14180 0.22 +0.0
3,238,695 - BPHYT_RS14180 0.31 -0.0
3,238,753 - BPHYT_RS14180 0.42 -1.1
3,238,983 - BPHYT_RS14180 0.87 -1.8
3,239,074 + -1.6
3,239,074 + -0.5
3,239,075 - -0.7
3,239,095 + +3.2
3,239,231 - BPHYT_RS14185 0.34 -0.1

Or see this region's nucleotide sequence