Experiment: Vogels_fungal_media 0.2X; growth supplemented with 0.4X LB
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BPHYT_RS01105 and BPHYT_RS01110 are separated by 114 nucleotides BPHYT_RS01110 and BPHYT_RS01115 are separated by 195 nucleotides BPHYT_RS01115 and BPHYT_RS01120 are separated by 139 nucleotides BPHYT_RS01120 and BPHYT_RS01125 are separated by 162 nucleotides
BPHYT_RS01105: BPHYT_RS01105 - hypothetical protein, at 255,197 to 255,916
_RS01105
BPHYT_RS01110: BPHYT_RS01110 - hypothetical protein, at 256,031 to 256,237
_RS01110
BPHYT_RS01115: BPHYT_RS01115 - hypothetical protein, at 256,433 to 256,678
_RS01115
BPHYT_RS01120: BPHYT_RS01120 - plasmid partitioning protein ParB, at 256,818 to 258,902
_RS01120
BPHYT_RS01125: BPHYT_RS01125 - integrase family protein, at 259,065 to 260,291
_RS01125
Position (kb) 256
257
258
259 Strain fitness (log2 ratio) -3
-2
-1
0
1 at 256.566 kb on + strand, within BPHYT_RS01115 at 256.584 kb on + strand, within BPHYT_RS01115 at 256.617 kb on + strand, within BPHYT_RS01115 at 256.681 kb on + strand at 256.681 kb on + strand at 256.681 kb on + strand at 256.712 kb on - strand at 256.809 kb on + strand at 256.810 kb on - strand at 257.932 kb on - strand, within BPHYT_RS01120 at 258.010 kb on + strand, within BPHYT_RS01120 at 258.271 kb on + strand, within BPHYT_RS01120 at 258.410 kb on - strand, within BPHYT_RS01120 at 258.611 kb on - strand, within BPHYT_RS01120 at 258.778 kb on + strand at 259.007 kb on + strand at 259.007 kb on + strand at 259.008 kb on - strand at 259.014 kb on - strand at 259.080 kb on + strand at 259.080 kb on + strand at 259.110 kb on + strand at 259.325 kb on - strand, within BPHYT_RS01125 at 259.325 kb on - strand, within BPHYT_RS01125 at 259.390 kb on + strand, within BPHYT_RS01125 at 259.391 kb on - strand, within BPHYT_RS01125 at 259.411 kb on - strand, within BPHYT_RS01125 at 259.466 kb on + strand, within BPHYT_RS01125 at 259.489 kb on + strand, within BPHYT_RS01125 at 259.489 kb on + strand, within BPHYT_RS01125 at 259.551 kb on + strand, within BPHYT_RS01125 at 259.552 kb on - strand, within BPHYT_RS01125 at 259.603 kb on + strand, within BPHYT_RS01125 at 259.622 kb on - strand, within BPHYT_RS01125 at 259.638 kb on - strand, within BPHYT_RS01125 at 259.692 kb on + strand, within BPHYT_RS01125 at 259.734 kb on - strand, within BPHYT_RS01125 at 259.848 kb on - strand, within BPHYT_RS01125 at 259.848 kb on - strand, within BPHYT_RS01125
Per-strain Table
Position Strand Gene LocusTag Fraction Vogels_fungal_media 0.2X; growth supplemented with 0.4X LB remove 256,566 + BPHYT_RS01115 0.54 +1.2 256,584 + BPHYT_RS01115 0.61 +0.3 256,617 + BPHYT_RS01115 0.75 -1.0 256,681 + -0.6 256,681 + +0.4 256,681 + -0.1 256,712 - -0.0 256,809 + -0.8 256,810 - +0.9 257,932 - BPHYT_RS01120 0.53 -0.8 258,010 + BPHYT_RS01120 0.57 -1.1 258,271 + BPHYT_RS01120 0.70 -0.9 258,410 - BPHYT_RS01120 0.76 -0.7 258,611 - BPHYT_RS01120 0.86 -1.5 258,778 + -1.6 259,007 + +0.5 259,007 + -3.0 259,008 - +0.5 259,014 - +0.3 259,080 + -0.3 259,080 + +0.8 259,110 + +1.2 259,325 - BPHYT_RS01125 0.21 -0.5 259,325 - BPHYT_RS01125 0.21 -0.7 259,390 + BPHYT_RS01125 0.26 -0.5 259,391 - BPHYT_RS01125 0.27 +0.2 259,411 - BPHYT_RS01125 0.28 +0.0 259,466 + BPHYT_RS01125 0.33 -0.3 259,489 + BPHYT_RS01125 0.35 -0.3 259,489 + BPHYT_RS01125 0.35 +0.4 259,551 + BPHYT_RS01125 0.40 +0.2 259,552 - BPHYT_RS01125 0.40 +0.2 259,603 + BPHYT_RS01125 0.44 -2.2 259,622 - BPHYT_RS01125 0.45 -0.1 259,638 - BPHYT_RS01125 0.47 +1.0 259,692 + BPHYT_RS01125 0.51 +0.7 259,734 - BPHYT_RS01125 0.55 -0.0 259,848 - BPHYT_RS01125 0.64 -0.1 259,848 - BPHYT_RS01125 0.64 +0.4
Or see this region's nucleotide sequence