Strain Fitness in Klebsiella michiganensis M5al around BWI76_RS20340

Experiment: Fungal_growth_media_mCAFEs_v1 with Vancomycin 0.01 mg/mL

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntBWI76_RS20335 and BWI76_RS20340 are separated by 257 nucleotidesBWI76_RS20340 and BWI76_RS20345 are separated by 291 nucleotidesBWI76_RS20345 and BWI76_RS20350 are separated by 136 nucleotides BWI76_RS20335: BWI76_RS20335 - zinc transporter, at 4,257,019 to 4,257,801 _RS20335 BWI76_RS20340: BWI76_RS20340 - histidine ABC transporter substrate-binding protein HisJ, at 4,258,059 to 4,258,841 _RS20340 BWI76_RS20345: BWI76_RS20345 - 3-octaprenyl-4-hydroxybenzoate carboxy-lyase, at 4,259,133 to 4,259,702 _RS20345 BWI76_RS20350: BWI76_RS20350 - amidophosphoribosyltransferase, at 4,259,839 to 4,261,356 _RS20350 Position (kb) 4258 4259Strain fitness (log2 ratio) -1 0 1 2at 4257.586 kb on - strand, within BWI76_RS20335at 4257.598 kb on + strand, within BWI76_RS20335at 4257.680 kb on + strand, within BWI76_RS20335at 4257.705 kb on - strand, within BWI76_RS20335at 4257.814 kb on - strandat 4258.239 kb on + strand, within BWI76_RS20340at 4258.417 kb on - strand, within BWI76_RS20340at 4258.419 kb on + strand, within BWI76_RS20340at 4258.829 kb on - strandat 4258.848 kb on + strand

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Per-strain Table

Position Strand Gene LocusTag Fraction Fungal_growth_media_mCAFEs_v1 with Vancomycin 0.01 mg/mL
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4,257,586 - BWI76_RS20335 0.72 -0.2
4,257,598 + BWI76_RS20335 0.74 +0.4
4,257,680 + BWI76_RS20335 0.84 -0.3
4,257,705 - BWI76_RS20335 0.88 +0.4
4,257,814 - +0.0
4,258,239 + BWI76_RS20340 0.23 +2.4
4,258,417 - BWI76_RS20340 0.46 +1.4
4,258,419 + BWI76_RS20340 0.46 +0.1
4,258,829 - +1.0
4,258,848 + -0.9

Or see this region's nucleotide sequence