Experiment: Fungal_growth_media_mCAFEs_v1 with Vancomycin 0.01 mg/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS12420 and BWI76_RS12425 are separated by 363 nucleotides BWI76_RS12425 and BWI76_RS12430 are separated by 169 nucleotides BWI76_RS12430 and BWI76_RS12435 are separated by 169 nucleotides
BWI76_RS12420: BWI76_RS12420 - peptide ABC transporter substrate-binding protein SapA, at 2,594,005 to 2,595,648
_RS12420
BWI76_RS12425: BWI76_RS12425 - hypothetical protein, at 2,596,012 to 2,596,296
_RS12425
BWI76_RS12430: BWI76_RS12430 - AraC family transcriptional regulator, at 2,596,466 to 2,598,772
_RS12430
BWI76_RS12435: BWI76_RS12435 - putative amino acid amidohydrolase, at 2,598,942 to 2,600,111
_RS12435
Position (kb)
2596
2597
2598
2599 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3
4 at 2595.881 kb on + strand at 2595.889 kb on - strand at 2595.889 kb on - strand at 2595.889 kb on - strand at 2596.004 kb on - strand at 2596.086 kb on - strand, within BWI76_RS12425 at 2596.105 kb on - strand, within BWI76_RS12425 at 2596.115 kb on - strand, within BWI76_RS12425 at 2596.244 kb on + strand, within BWI76_RS12425 at 2596.252 kb on - strand, within BWI76_RS12425 at 2596.594 kb on + strand at 2596.728 kb on + strand, within BWI76_RS12430 at 2597.157 kb on - strand, within BWI76_RS12430 at 2597.377 kb on - strand, within BWI76_RS12430 at 2597.428 kb on + strand, within BWI76_RS12430 at 2597.566 kb on - strand, within BWI76_RS12430 at 2597.648 kb on - strand, within BWI76_RS12430 at 2597.733 kb on + strand, within BWI76_RS12430 at 2597.844 kb on + strand, within BWI76_RS12430 at 2597.875 kb on - strand, within BWI76_RS12430 at 2597.889 kb on + strand, within BWI76_RS12430 at 2598.015 kb on - strand, within BWI76_RS12430 at 2598.102 kb on + strand, within BWI76_RS12430 at 2598.151 kb on + strand, within BWI76_RS12430 at 2598.151 kb on + strand, within BWI76_RS12430 at 2598.344 kb on - strand, within BWI76_RS12430 at 2598.459 kb on - strand, within BWI76_RS12430 at 2598.543 kb on - strand at 2598.719 kb on + strand at 2598.920 kb on + strand at 2598.969 kb on - strand at 2599.137 kb on - strand, within BWI76_RS12435 at 2599.211 kb on + strand, within BWI76_RS12435 at 2599.219 kb on - strand, within BWI76_RS12435 at 2599.285 kb on - strand, within BWI76_RS12435 at 2599.366 kb on - strand, within BWI76_RS12435 at 2599.366 kb on - strand, within BWI76_RS12435 at 2599.366 kb on - strand, within BWI76_RS12435 at 2599.381 kb on - strand, within BWI76_RS12435 at 2599.657 kb on + strand, within BWI76_RS12435 at 2599.664 kb on + strand, within BWI76_RS12435
Per-strain Table
Position Strand Gene LocusTag Fraction Fungal_growth_media_mCAFEs_v1 with Vancomycin 0.01 mg/mL remove 2,595,881 + -2.9 2,595,889 - -0.7 2,595,889 - +0.3 2,595,889 - +0.7 2,596,004 - +2.3 2,596,086 - BWI76_RS12425 0.26 +0.2 2,596,105 - BWI76_RS12425 0.33 +0.4 2,596,115 - BWI76_RS12425 0.36 +0.9 2,596,244 + BWI76_RS12425 0.81 -0.7 2,596,252 - BWI76_RS12425 0.84 +2.0 2,596,594 + +0.2 2,596,728 + BWI76_RS12430 0.11 +0.2 2,597,157 - BWI76_RS12430 0.30 -0.4 2,597,377 - BWI76_RS12430 0.39 +1.1 2,597,428 + BWI76_RS12430 0.42 +0.1 2,597,566 - BWI76_RS12430 0.48 +1.6 2,597,648 - BWI76_RS12430 0.51 +0.8 2,597,733 + BWI76_RS12430 0.55 +0.7 2,597,844 + BWI76_RS12430 0.60 +2.8 2,597,875 - BWI76_RS12430 0.61 -0.3 2,597,889 + BWI76_RS12430 0.62 +1.0 2,598,015 - BWI76_RS12430 0.67 +2.2 2,598,102 + BWI76_RS12430 0.71 -0.2 2,598,151 + BWI76_RS12430 0.73 +1.2 2,598,151 + BWI76_RS12430 0.73 +0.0 2,598,344 - BWI76_RS12430 0.81 +0.2 2,598,459 - BWI76_RS12430 0.86 +0.1 2,598,543 - +1.3 2,598,719 + +1.2 2,598,920 + +0.7 2,598,969 - +0.8 2,599,137 - BWI76_RS12435 0.17 +0.3 2,599,211 + BWI76_RS12435 0.23 +4.5 2,599,219 - BWI76_RS12435 0.24 +0.5 2,599,285 - BWI76_RS12435 0.29 -0.2 2,599,366 - BWI76_RS12435 0.36 -3.1 2,599,366 - BWI76_RS12435 0.36 +0.5 2,599,366 - BWI76_RS12435 0.36 +2.8 2,599,381 - BWI76_RS12435 0.38 -1.1 2,599,657 + BWI76_RS12435 0.61 +0.4 2,599,664 + BWI76_RS12435 0.62 -0.0
Or see this region's nucleotide sequence