Experiment: Fungal_growth_media_mCAFEs_v1 with Vancomycin 0.01 mg/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS10305 and BWI76_RS10310 are separated by 100 nucleotides BWI76_RS10310 and BWI76_RS10315 are separated by 201 nucleotides BWI76_RS10315 and BWI76_RS10320 are separated by 79 nucleotides
BWI76_RS10305: BWI76_RS10305 - transposase, at 2,182,331 to 2,182,927
_RS10305
BWI76_RS10310: BWI76_RS10310 - DNA-binding transcriptional regulator RamA, at 2,183,028 to 2,183,372
_RS10310
BWI76_RS10315: BWI76_RS10315 - MFS transporter, at 2,183,574 to 2,184,866
_RS10315
BWI76_RS10320: BWI76_RS10320 - alpha-glucosidase/alpha-galactosidase, at 2,184,946 to 2,186,253
_RS10320
Position (kb)
2183
2184
2185 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 2182.622 kb on + strand, within BWI76_RS10305 at 2182.622 kb on + strand, within BWI76_RS10305 at 2182.640 kb on + strand, within BWI76_RS10305 at 2182.651 kb on + strand, within BWI76_RS10305 at 2182.681 kb on + strand, within BWI76_RS10305 at 2182.681 kb on + strand, within BWI76_RS10305 at 2182.681 kb on + strand, within BWI76_RS10305 at 2182.705 kb on + strand, within BWI76_RS10305 at 2182.705 kb on + strand, within BWI76_RS10305 at 2182.711 kb on + strand, within BWI76_RS10305 at 2182.751 kb on + strand, within BWI76_RS10305 at 2182.751 kb on + strand, within BWI76_RS10305 at 2182.772 kb on + strand, within BWI76_RS10305 at 2182.930 kb on - strand at 2182.930 kb on - strand at 2182.943 kb on + strand at 2183.093 kb on + strand, within BWI76_RS10310 at 2183.098 kb on + strand, within BWI76_RS10310 at 2183.098 kb on + strand, within BWI76_RS10310 at 2183.098 kb on + strand, within BWI76_RS10310 at 2183.166 kb on + strand, within BWI76_RS10310 at 2183.170 kb on - strand, within BWI76_RS10310 at 2183.183 kb on + strand, within BWI76_RS10310 at 2183.183 kb on - strand, within BWI76_RS10310 at 2183.262 kb on - strand, within BWI76_RS10310 at 2183.272 kb on + strand, within BWI76_RS10310 at 2183.272 kb on + strand, within BWI76_RS10310 at 2183.280 kb on - strand, within BWI76_RS10310 at 2183.280 kb on - strand, within BWI76_RS10310 at 2183.280 kb on - strand, within BWI76_RS10310 at 2183.291 kb on - strand, within BWI76_RS10310 at 2183.291 kb on - strand, within BWI76_RS10310 at 2183.384 kb on - strand at 2183.390 kb on + strand at 2183.397 kb on + strand at 2183.398 kb on - strand at 2183.405 kb on - strand at 2183.405 kb on - strand at 2183.405 kb on - strand at 2183.405 kb on - strand at 2183.406 kb on + strand at 2183.444 kb on + strand at 2183.452 kb on - strand at 2183.465 kb on + strand at 2183.473 kb on - strand at 2183.473 kb on - strand at 2183.473 kb on - strand at 2183.473 kb on - strand at 2183.473 kb on - strand at 2183.473 kb on - strand at 2183.473 kb on - strand at 2183.572 kb on + strand at 2183.580 kb on - strand at 2183.659 kb on + strand at 2183.702 kb on - strand at 2183.702 kb on - strand at 2183.720 kb on + strand, within BWI76_RS10315 at 2183.728 kb on - strand, within BWI76_RS10315 at 2183.759 kb on - strand, within BWI76_RS10315 at 2183.770 kb on - strand, within BWI76_RS10315 at 2183.777 kb on - strand, within BWI76_RS10315 at 2183.777 kb on - strand, within BWI76_RS10315 at 2183.777 kb on - strand, within BWI76_RS10315 at 2183.777 kb on - strand, within BWI76_RS10315 at 2183.782 kb on + strand, within BWI76_RS10315 at 2183.790 kb on - strand, within BWI76_RS10315 at 2183.839 kb on + strand, within BWI76_RS10315 at 2183.839 kb on + strand, within BWI76_RS10315 at 2183.847 kb on - strand at 2184.087 kb on + strand, within BWI76_RS10315 at 2184.097 kb on + strand, within BWI76_RS10315 at 2184.325 kb on + strand, within BWI76_RS10315 at 2184.325 kb on + strand, within BWI76_RS10315 at 2184.332 kb on - strand, within BWI76_RS10315 at 2184.535 kb on - strand, within BWI76_RS10315 at 2184.717 kb on + strand, within BWI76_RS10315 at 2184.752 kb on + strand at 2184.760 kb on - strand at 2184.934 kb on + strand at 2185.055 kb on + strand at 2185.058 kb on - strand at 2185.224 kb on - strand, within BWI76_RS10320 at 2185.402 kb on - strand at 2185.475 kb on + strand, within BWI76_RS10320 at 2185.547 kb on + strand, within BWI76_RS10320 at 2185.569 kb on + strand, within BWI76_RS10320 at 2185.710 kb on + strand, within BWI76_RS10320 at 2185.730 kb on - strand, within BWI76_RS10320 at 2185.796 kb on + strand, within BWI76_RS10320
Per-strain Table
Position Strand Gene LocusTag Fraction Fungal_growth_media_mCAFEs_v1 with Vancomycin 0.01 mg/mL remove 2,182,622 + BWI76_RS10305 0.49 -1.4 2,182,622 + BWI76_RS10305 0.49 -1.2 2,182,640 + BWI76_RS10305 0.52 +0.1 2,182,651 + BWI76_RS10305 0.54 -1.9 2,182,681 + BWI76_RS10305 0.59 -1.6 2,182,681 + BWI76_RS10305 0.59 -1.8 2,182,681 + BWI76_RS10305 0.59 -0.6 2,182,705 + BWI76_RS10305 0.63 -1.8 2,182,705 + BWI76_RS10305 0.63 -1.2 2,182,711 + BWI76_RS10305 0.64 -3.3 2,182,751 + BWI76_RS10305 0.70 -2.3 2,182,751 + BWI76_RS10305 0.70 -0.6 2,182,772 + BWI76_RS10305 0.74 -0.8 2,182,930 - +1.2 2,182,930 - -0.6 2,182,943 + -0.8 2,183,093 + BWI76_RS10310 0.19 -1.0 2,183,098 + BWI76_RS10310 0.20 -1.6 2,183,098 + BWI76_RS10310 0.20 +0.5 2,183,098 + BWI76_RS10310 0.20 +1.0 2,183,166 + BWI76_RS10310 0.40 +0.0 2,183,170 - BWI76_RS10310 0.41 -0.7 2,183,183 + BWI76_RS10310 0.45 -2.1 2,183,183 - BWI76_RS10310 0.45 -0.1 2,183,262 - BWI76_RS10310 0.68 -0.6 2,183,272 + BWI76_RS10310 0.71 -2.0 2,183,272 + BWI76_RS10310 0.71 -0.2 2,183,280 - BWI76_RS10310 0.73 +1.0 2,183,280 - BWI76_RS10310 0.73 +0.0 2,183,280 - BWI76_RS10310 0.73 +1.0 2,183,291 - BWI76_RS10310 0.76 -2.7 2,183,291 - BWI76_RS10310 0.76 +1.0 2,183,384 - +0.2 2,183,390 + -0.3 2,183,397 + -2.2 2,183,398 - +2.4 2,183,405 - -0.4 2,183,405 - -1.1 2,183,405 - +0.4 2,183,405 - +0.4 2,183,406 + -1.0 2,183,444 + -1.8 2,183,452 - -1.6 2,183,465 + -1.6 2,183,473 - -2.0 2,183,473 - -0.1 2,183,473 - +0.0 2,183,473 - -0.8 2,183,473 - -0.7 2,183,473 - -2.4 2,183,473 - -0.6 2,183,572 + -2.4 2,183,580 - -0.8 2,183,659 + +0.7 2,183,702 - +0.2 2,183,702 - +0.0 2,183,720 + BWI76_RS10315 0.11 -1.6 2,183,728 - BWI76_RS10315 0.12 -2.1 2,183,759 - BWI76_RS10315 0.14 +0.8 2,183,770 - BWI76_RS10315 0.15 +0.2 2,183,777 - BWI76_RS10315 0.16 -2.7 2,183,777 - BWI76_RS10315 0.16 +0.0 2,183,777 - BWI76_RS10315 0.16 -0.7 2,183,777 - BWI76_RS10315 0.16 +0.0 2,183,782 + BWI76_RS10315 0.16 +1.6 2,183,790 - BWI76_RS10315 0.17 +0.1 2,183,839 + BWI76_RS10315 0.20 +1.1 2,183,839 + BWI76_RS10315 0.20 -0.1 2,183,847 - +1.0 2,184,087 + BWI76_RS10315 0.40 -0.7 2,184,097 + BWI76_RS10315 0.40 -0.8 2,184,325 + BWI76_RS10315 0.58 +0.3 2,184,325 + BWI76_RS10315 0.58 +2.3 2,184,332 - BWI76_RS10315 0.59 -0.4 2,184,535 - BWI76_RS10315 0.74 +0.3 2,184,717 + BWI76_RS10315 0.88 -1.0 2,184,752 + -0.8 2,184,760 - -2.0 2,184,934 + -0.9 2,185,055 + -0.0 2,185,058 - -1.9 2,185,224 - BWI76_RS10320 0.21 +1.3 2,185,402 - +1.0 2,185,475 + BWI76_RS10320 0.40 +2.7 2,185,547 + BWI76_RS10320 0.46 +1.3 2,185,569 + BWI76_RS10320 0.48 +0.8 2,185,710 + BWI76_RS10320 0.58 +0.1 2,185,730 - BWI76_RS10320 0.60 -2.0 2,185,796 + BWI76_RS10320 0.65 +0.2
Or see this region's nucleotide sequence