Strain Fitness in Klebsiella michiganensis M5al around BWI76_RS06400

Experiment: Fungal_growth_media_mCAFEs_v1 with Vancomycin 0.01 mg/mL

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntBWI76_RS06395 and BWI76_RS06400 are separated by 58 nucleotidesBWI76_RS06400 and BWI76_RS06405 overlap by 38 nucleotides BWI76_RS06395: BWI76_RS06395 - tRNA 4-thiouridine(8) synthase ThiI, at 1,343,831 to 1,345,279 _RS06395 BWI76_RS06400: BWI76_RS06400 - oxidative-stress-resistance chaperone, at 1,345,338 to 1,345,928 _RS06400 BWI76_RS06405: BWI76_RS06405 - 2-dehydropantoate 2-reductase, at 1,345,891 to 1,346,802 _RS06405 Position (kb) 1345 1346Strain fitness (log2 ratio) -3 -2 -1 0 1 2 3at 1344.507 kb on + strand, within BWI76_RS06395at 1345.113 kb on - strand, within BWI76_RS06395at 1345.420 kb on - strand, within BWI76_RS06400at 1345.780 kb on - strandat 1345.835 kb on + strand, within BWI76_RS06400at 1346.458 kb on - strand, within BWI76_RS06405at 1346.480 kb on - strand, within BWI76_RS06405at 1346.537 kb on - strand, within BWI76_RS06405at 1346.719 kb on + strandat 1346.727 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction Fungal_growth_media_mCAFEs_v1 with Vancomycin 0.01 mg/mL
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1,344,507 + BWI76_RS06395 0.47 -2.8
1,345,113 - BWI76_RS06395 0.88 -2.0
1,345,420 - BWI76_RS06400 0.14 +3.2
1,345,780 - +0.0
1,345,835 + BWI76_RS06400 0.84 +0.6
1,346,458 - BWI76_RS06405 0.62 +1.0
1,346,480 - BWI76_RS06405 0.65 -0.6
1,346,537 - BWI76_RS06405 0.71 +1.2
1,346,719 + -1.1
1,346,727 - -0.3

Or see this region's nucleotide sequence