Experiment: Fungal_growth_media_mCAFEs_v1 with Zinc 2 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS21160 and BWI76_RS21165 are separated by 177 nucleotides BWI76_RS21165 and BWI76_RS21170 are separated by 233 nucleotides BWI76_RS21170 and BWI76_RS21175 are separated by 120 nucleotides BWI76_RS21175 and BWI76_RS21180 are separated by 54 nucleotides
BWI76_RS21160: BWI76_RS21160 - IscS subfamily cysteine desulfurase, at 4,430,056 to 4,431,270
_RS21160
BWI76_RS21165: BWI76_RS21165 - Fe-S cluster assembly transcriptional regulator IscR, at 4,431,448 to 4,431,939
_RS21165
BWI76_RS21170: BWI76_RS21170 - tRNA (cytosine(32)/uridine(32)-2'-O)-methyltransferase TrmJ, at 4,432,173 to 4,432,904
_RS21170
BWI76_RS21175: BWI76_RS21175 - inositol monophosphatase, at 4,433,025 to 4,433,828
_RS21175
BWI76_RS21180: BWI76_RS21180 - nickel transporter, at 4,433,883 to 4,434,863
_RS21180
Position (kb) 4432
4433 Strain fitness (log2 ratio) -2
-1
0
1
2
3 at 4431.572 kb on - strand, within BWI76_RS21165 at 4432.156 kb on + strand at 4432.639 kb on + strand, within BWI76_RS21170 at 4432.647 kb on - strand, within BWI76_RS21170 at 4432.809 kb on - strand, within BWI76_RS21170 at 4432.820 kb on - strand, within BWI76_RS21170 at 4432.851 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Fungal_growth_media_mCAFEs_v1 with Zinc 2 mM remove 4,431,572 - BWI76_RS21165 0.25 -2.4 4,432,156 + +3.6 4,432,639 + BWI76_RS21170 0.64 -0.3 4,432,647 - BWI76_RS21170 0.65 +0.2 4,432,809 - BWI76_RS21170 0.87 +0.5 4,432,820 - BWI76_RS21170 0.88 +1.1 4,432,851 - +0.6
Or see this region's nucleotide sequence