Experiment: Fungal_growth_media_mCAFEs_v1 with Zinc 2 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS20610 and BWI76_RS20615 are separated by 89 nucleotides BWI76_RS20615 and BWI76_RS20620 overlap by 4 nucleotides BWI76_RS20620 and BWI76_RS20625 overlap by 8 nucleotides
BWI76_RS20610: BWI76_RS20610 - LysR family transcriptional regulator, at 4,312,740 to 4,313,666
_RS20610
BWI76_RS20615: BWI76_RS20615 - sodium symporter, at 4,313,756 to 4,314,754
_RS20615
BWI76_RS20620: BWI76_RS20620 - negative regulator, at 4,314,751 to 4,314,969
_RS20620
BWI76_RS20625: BWI76_RS20625 - DNA ligase (NAD(+)) LigA, at 4,314,962 to 4,316,986
_RS20625
Position (kb) 4313
4314
4315 Strain fitness (log2 ratio) -2
-1
0
1 at 4313.394 kb on - strand, within BWI76_RS20610 at 4313.416 kb on + strand, within BWI76_RS20610 at 4313.476 kb on + strand, within BWI76_RS20610 at 4313.493 kb on - strand, within BWI76_RS20610 at 4313.530 kb on - strand, within BWI76_RS20610 at 4313.582 kb on + strand at 4313.590 kb on - strand at 4313.602 kb on - strand at 4313.924 kb on - strand, within BWI76_RS20615 at 4314.152 kb on + strand, within BWI76_RS20615 at 4314.227 kb on + strand, within BWI76_RS20615 at 4314.234 kb on + strand, within BWI76_RS20615 at 4314.242 kb on - strand, within BWI76_RS20615 at 4314.604 kb on + strand, within BWI76_RS20615 at 4314.673 kb on - strand at 4314.769 kb on - strand at 4314.769 kb on - strand at 4314.784 kb on + strand, within BWI76_RS20620
Per-strain Table
Position Strand Gene LocusTag Fraction Fungal_growth_media_mCAFEs_v1 with Zinc 2 mM remove 4,313,394 - BWI76_RS20610 0.71 -1.8 4,313,416 + BWI76_RS20610 0.73 +0.2 4,313,476 + BWI76_RS20610 0.79 -1.1 4,313,493 - BWI76_RS20610 0.81 +0.7 4,313,530 - BWI76_RS20610 0.85 -2.2 4,313,582 + +1.1 4,313,590 - +1.0 4,313,602 - +0.0 4,313,924 - BWI76_RS20615 0.17 -2.2 4,314,152 + BWI76_RS20615 0.40 +0.2 4,314,227 + BWI76_RS20615 0.47 -0.1 4,314,234 + BWI76_RS20615 0.48 -1.4 4,314,242 - BWI76_RS20615 0.49 +0.1 4,314,604 + BWI76_RS20615 0.85 -1.8 4,314,673 - -1.2 4,314,769 - -1.7 4,314,769 - +0.9 4,314,784 + BWI76_RS20620 0.15 +0.0
Or see this region's nucleotide sequence