Experiment: Fungal_growth_media_mCAFEs_v1 with Zinc 2 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS02485 and BWI76_RS02490 are separated by 286 nucleotides BWI76_RS02490 and BWI76_RS02495 are separated by 23 nucleotides BWI76_RS02495 and BWI76_RS02500 are separated by 24 nucleotides BWI76_RS02500 and BWI76_RS02505 are separated by 270 nucleotides BWI76_RS02505 and BWI76_RS02510 overlap by 2 nucleotides
BWI76_RS02485: BWI76_RS02485 - polar amino acid ABC transporter (PAAT) family, periplasmic amino acid-binding protein, at 510,980 to 511,711
_RS02485
BWI76_RS02490: BWI76_RS02490 - tRNA-Gly, at 511,998 to 512,073
_RS02490
BWI76_RS02495: BWI76_RS02495 - tRNA-Gly, at 512,097 to 512,172
_RS02495
BWI76_RS02500: BWI76_RS02500 - tRNA-Gly, at 512,197 to 512,272
_RS02500
BWI76_RS02505: BWI76_RS02505 - tRNA epoxyqueuosine(34) reductase QueG, at 512,543 to 513,682
_RS02505
BWI76_RS02510: BWI76_RS02510 - bifunctional ADP-dependent NAD(P)H-hydrate dehydratase/NAD(P)H-hydrate epimerase, at 513,681 to 515,207
_RS02510
Position (kb) 512
513
514 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1
2
3
4 at 511.550 kb on - strand, within BWI76_RS02485 at 511.559 kb on + strand, within BWI76_RS02485 at 512.582 kb on - strand at 512.584 kb on + strand at 512.689 kb on - strand, within BWI76_RS02505 at 512.733 kb on + strand, within BWI76_RS02505 at 512.733 kb on + strand, within BWI76_RS02505 at 512.733 kb on + strand, within BWI76_RS02505 at 512.868 kb on + strand, within BWI76_RS02505 at 513.045 kb on + strand, within BWI76_RS02505 at 513.201 kb on - strand, within BWI76_RS02505 at 513.203 kb on + strand, within BWI76_RS02505 at 513.354 kb on - strand, within BWI76_RS02505 at 513.354 kb on - strand, within BWI76_RS02505 at 513.389 kb on - strand, within BWI76_RS02505 at 513.481 kb on + strand, within BWI76_RS02505 at 513.567 kb on - strand, within BWI76_RS02505 at 513.610 kb on + strand at 513.617 kb on + strand at 513.622 kb on + strand at 513.625 kb on - strand at 513.712 kb on + strand at 513.752 kb on - strand at 513.778 kb on + strand at 513.778 kb on + strand at 513.778 kb on + strand at 513.778 kb on + strand at 513.786 kb on - strand at 513.913 kb on - strand, within BWI76_RS02510 at 513.935 kb on - strand, within BWI76_RS02510 at 514.099 kb on - strand at 514.101 kb on + strand, within BWI76_RS02510 at 514.101 kb on + strand, within BWI76_RS02510 at 514.104 kb on - strand, within BWI76_RS02510 at 514.109 kb on - strand, within BWI76_RS02510 at 514.156 kb on - strand, within BWI76_RS02510 at 514.194 kb on + strand, within BWI76_RS02510 at 514.203 kb on + strand, within BWI76_RS02510 at 514.250 kb on - strand, within BWI76_RS02510 at 514.310 kb on - strand, within BWI76_RS02510 at 514.327 kb on + strand, within BWI76_RS02510 at 514.639 kb on + strand, within BWI76_RS02510 at 514.666 kb on + strand, within BWI76_RS02510
Per-strain Table
Position Strand Gene LocusTag Fraction Fungal_growth_media_mCAFEs_v1 with Zinc 2 mM remove 511,550 - BWI76_RS02485 0.78 +0.4 511,559 + BWI76_RS02485 0.79 -0.7 512,582 - -4.3 512,584 + -1.3 512,689 - BWI76_RS02505 0.13 +0.3 512,733 + BWI76_RS02505 0.17 -2.1 512,733 + BWI76_RS02505 0.17 -0.4 512,733 + BWI76_RS02505 0.17 -0.5 512,868 + BWI76_RS02505 0.29 -1.7 513,045 + BWI76_RS02505 0.44 -1.1 513,201 - BWI76_RS02505 0.58 -0.7 513,203 + BWI76_RS02505 0.58 +0.1 513,354 - BWI76_RS02505 0.71 +0.1 513,354 - BWI76_RS02505 0.71 -3.5 513,389 - BWI76_RS02505 0.74 -0.3 513,481 + BWI76_RS02505 0.82 -3.3 513,567 - BWI76_RS02505 0.90 +0.1 513,610 + +0.1 513,617 + +0.7 513,622 + +2.1 513,625 - -2.9 513,712 + -0.3 513,752 - +0.3 513,778 + +0.1 513,778 + -2.1 513,778 + -0.5 513,778 + +0.9 513,786 - -0.2 513,913 - BWI76_RS02510 0.15 -1.2 513,935 - BWI76_RS02510 0.17 -2.1 514,099 - +1.1 514,101 + BWI76_RS02510 0.28 -0.3 514,101 + BWI76_RS02510 0.28 +0.3 514,104 - BWI76_RS02510 0.28 +0.2 514,109 - BWI76_RS02510 0.28 +0.2 514,156 - BWI76_RS02510 0.31 +0.1 514,194 + BWI76_RS02510 0.34 +4.1 514,203 + BWI76_RS02510 0.34 -2.4 514,250 - BWI76_RS02510 0.37 +1.6 514,310 - BWI76_RS02510 0.41 -2.3 514,327 + BWI76_RS02510 0.42 -1.3 514,639 + BWI76_RS02510 0.63 -0.1 514,666 + BWI76_RS02510 0.65 +2.3
Or see this region's nucleotide sequence