Strain Fitness in Burkholderia phytofirmans PsJN around BPHYT_RS25410

Experiment: Supernatant; Trichoderma atroviride IMI grown in Vogels_fungal_media, non-filtered; 0.2X; growth supplemented with 0.4X LB

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntBPHYT_RS25405 and BPHYT_RS25410 are separated by 358 nucleotidesBPHYT_RS25410 and BPHYT_RS25415 are separated by 76 nucleotidesBPHYT_RS25415 and BPHYT_RS25420 are separated by 154 nucleotides BPHYT_RS25405: BPHYT_RS25405 - CoA-transferase, at 1,277,126 to 1,278,196 _RS25405 BPHYT_RS25410: BPHYT_RS25410 - porin, at 1,278,555 to 1,279,670 _RS25410 BPHYT_RS25415: BPHYT_RS25415 - 3-hydroxy-2-methylbutyryl-CoA dehydrogenase, at 1,279,747 to 1,280,493 _RS25415 BPHYT_RS25420: BPHYT_RS25420 - hypothetical protein, at 1,280,648 to 1,280,842 _RS25420 Position (kb) 1278 1279 1280Strain fitness (log2 ratio) -1 0 1at 1277.686 kb on - strand, within BPHYT_RS25405at 1277.785 kb on - strand, within BPHYT_RS25405at 1277.850 kb on + strand, within BPHYT_RS25405at 1278.292 kb on - strandat 1278.560 kb on + strandat 1278.639 kb on + strandat 1278.843 kb on + strand, within BPHYT_RS25410at 1278.906 kb on + strand, within BPHYT_RS25410at 1279.037 kb on + strand, within BPHYT_RS25410at 1279.087 kb on - strand, within BPHYT_RS25410at 1279.333 kb on - strand, within BPHYT_RS25410at 1279.565 kb on + strandat 1279.604 kb on + strandat 1279.612 kb on + strand

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Per-strain Table

Position Strand Gene LocusTag Fraction Supernatant; Trichoderma atroviride IMI grown in Vogels_fungal_media, non-filtered; 0.2X; growth supplemented with 0.4X LB
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1,277,686 - BPHYT_RS25405 0.52 -0.5
1,277,785 - BPHYT_RS25405 0.62 +0.4
1,277,850 + BPHYT_RS25405 0.68 +1.1
1,278,292 - +0.4
1,278,560 + +1.7
1,278,639 + -0.3
1,278,843 + BPHYT_RS25410 0.26 -0.4
1,278,906 + BPHYT_RS25410 0.31 +1.0
1,279,037 + BPHYT_RS25410 0.43 +0.8
1,279,087 - BPHYT_RS25410 0.48 +1.3
1,279,333 - BPHYT_RS25410 0.70 +1.4
1,279,565 + -0.2
1,279,604 + +0.0
1,279,612 + -0.2

Or see this region's nucleotide sequence