Experiment: Supernatant; Trichoderma atroviride IMI grown in Vogels_fungal_media, non-filtered; 0.2X; growth supplemented with 0.4X LB
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BPHYT_RS20960 and BPHYT_RS20965 are separated by 232 nucleotides BPHYT_RS20965 and BPHYT_RS20970 are separated by 70 nucleotides BPHYT_RS20970 and BPHYT_RS20975 are separated by 138 nucleotides
BPHYT_RS20960: BPHYT_RS20960 - dihydrofolate reductase, at 262,759 to 263,721
_RS20960
BPHYT_RS20965: BPHYT_RS20965 - photosystem reaction center subunit H, at 263,954 to 264,427
_RS20965
BPHYT_RS20970: BPHYT_RS20970 - hypothetical protein, at 264,498 to 264,728
_RS20970
BPHYT_RS20975: BPHYT_RS20975 - haloacid dehalogenase, at 264,867 to 265,595
_RS20975
Position (kb) 264
265 Strain fitness (log2 ratio) -1
0
1
2 at 263.578 kb on - strand, within BPHYT_RS20960 at 263.943 kb on - strand at 263.946 kb on - strand at 264.031 kb on - strand, within BPHYT_RS20965 at 264.452 kb on - strand at 264.659 kb on + strand, within BPHYT_RS20970 at 264.855 kb on + strand at 265.096 kb on + strand, within BPHYT_RS20975 at 265.096 kb on + strand, within BPHYT_RS20975 at 265.417 kb on - strand, within BPHYT_RS20975 at 265.696 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Supernatant; Trichoderma atroviride IMI grown in Vogels_fungal_media, non-filtered; 0.2X; growth supplemented with 0.4X LB remove 263,578 - BPHYT_RS20960 0.85 +1.3 263,943 - +1.4 263,946 - +2.4 264,031 - BPHYT_RS20965 0.16 -0.0 264,452 - -0.1 264,659 + BPHYT_RS20970 0.70 +1.3 264,855 + +1.7 265,096 + BPHYT_RS20975 0.31 +0.2 265,096 + BPHYT_RS20975 0.31 -0.4 265,417 - BPHYT_RS20975 0.75 +0.1 265,696 + +0.8
Or see this region's nucleotide sequence