Experiment: Supernatant; Trichoderma atroviride IMI grown in Vogels_fungal_media, non-filtered; 0.5X; growth supplemented with 0.4X LB
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BPHYT_RS20080 and BPHYT_RS20085 are separated by 16 nucleotides BPHYT_RS20085 and BPHYT_RS20090 are separated by 40 nucleotides BPHYT_RS20090 and BPHYT_RS20095 are separated by 249 nucleotides BPHYT_RS20095 and BPHYT_RS20100 overlap by 11 nucleotides
BPHYT_RS20080: BPHYT_RS20080 - ABC transporter permease, at 48,178 to 49,041
_RS20080
BPHYT_RS20085: BPHYT_RS20085 - mannosyltransferase, at 49,058 to 49,852
_RS20085
BPHYT_RS20090: BPHYT_RS20090 - nitrate ABC transporter substrate-binding protein, at 49,893 to 50,912
_RS20090
BPHYT_RS20095: BPHYT_RS20095 - transcriptional regulator, at 51,162 to 51,839
_RS20095
BPHYT_RS20100: BPHYT_RS20100 - sensor histidine kinase, at 51,829 to 53,223
_RS20100
Position (kb) 49
50
51 Strain fitness (log2 ratio) -1
0
1 at 49.041 kb on + strand at 49.042 kb on - strand at 49.519 kb on - strand, within BPHYT_RS20085 at 49.666 kb on + strand, within BPHYT_RS20085 at 49.894 kb on + strand at 50.102 kb on - strand, within BPHYT_RS20090 at 50.985 kb on + strand at 51.545 kb on - strand, within BPHYT_RS20095 at 51.891 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Supernatant; Trichoderma atroviride IMI grown in Vogels_fungal_media, non-filtered; 0.5X; growth supplemented with 0.4X LB remove 49,041 + -0.3 49,042 - -0.2 49,519 - BPHYT_RS20085 0.58 -0.5 49,666 + BPHYT_RS20085 0.76 +0.6 49,894 + +0.5 50,102 - BPHYT_RS20090 0.20 -0.1 50,985 + -0.4 51,545 - BPHYT_RS20095 0.56 -1.3 51,891 - -1.4
Or see this region's nucleotide sequence