Experiment: Fungal_growth_media_mCAFEs_v1 with Supernatant; Trichoderma harzianum M10 grown in Fungal_growth_media_mCAFEs_v2, filtered and pH-adjusted 0.8 x and biotin 0.32 ug/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS26100 and BWI76_RS26105 overlap by 92 nucleotides BWI76_RS26105 and BWI76_RS26110 overlap by 11 nucleotides BWI76_RS26110 and BWI76_RS26115 overlap by 11 nucleotides BWI76_RS26115 and BWI76_RS26120 overlap by 4 nucleotides BWI76_RS26120 and BWI76_RS26125 are separated by 117 nucleotides
BWI76_RS26100: BWI76_RS26100 - DNA transporter HofQ, at 5,392,117 to 5,393,358
_RS26100
BWI76_RS26105: BWI76_RS26105 - hypothetical protein, at 5,393,267 to 5,393,674
_RS26105
BWI76_RS26110: BWI76_RS26110 - hypothetical protein, at 5,393,664 to 5,394,104
_RS26110
BWI76_RS26115: BWI76_RS26115 - hypothetical protein, at 5,394,094 to 5,394,627
_RS26115
BWI76_RS26120: BWI76_RS26120 - hypothetical protein, at 5,394,624 to 5,395,415
_RS26120
BWI76_RS26125: BWI76_RS26125 - carboxypeptidase/penicillin-binding protein 1A, at 5,395,533 to 5,398,091
_RS26125
Position (kb) 5394
5395 Strain fitness (log2 ratio) -2
-1
0
1
2 at 5393.099 kb on - strand, within BWI76_RS26100 at 5393.136 kb on - strand, within BWI76_RS26100 at 5393.172 kb on + strand, within BWI76_RS26100 at 5393.196 kb on + strand, within BWI76_RS26100 at 5393.465 kb on - strand, within BWI76_RS26105 at 5393.465 kb on - strand, within BWI76_RS26105 at 5393.515 kb on - strand, within BWI76_RS26105 at 5393.515 kb on - strand, within BWI76_RS26105 at 5393.967 kb on - strand, within BWI76_RS26110 at 5394.013 kb on - strand, within BWI76_RS26110 at 5394.016 kb on + strand, within BWI76_RS26110 at 5394.025 kb on + strand, within BWI76_RS26110 at 5394.175 kb on - strand, within BWI76_RS26115 at 5394.258 kb on + strand, within BWI76_RS26115 at 5394.288 kb on + strand, within BWI76_RS26115 at 5394.440 kb on + strand, within BWI76_RS26115 at 5394.448 kb on - strand, within BWI76_RS26115 at 5394.448 kb on - strand, within BWI76_RS26115 at 5394.448 kb on - strand, within BWI76_RS26115 at 5394.622 kb on + strand at 5394.664 kb on - strand at 5394.887 kb on + strand, within BWI76_RS26120 at 5395.067 kb on + strand, within BWI76_RS26120 at 5395.315 kb on - strand, within BWI76_RS26120 at 5395.322 kb on + strand, within BWI76_RS26120 at 5395.471 kb on + strand at 5395.580 kb on + strand at 5395.580 kb on + strand at 5395.588 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Fungal_growth_media_mCAFEs_v1 with Supernatant; Trichoderma harzianum M10 grown in Fungal_growth_media_mCAFEs_v2, filtered and pH-adjusted 0.8 x and biotin 0.32 ug/ml remove 5,393,099 - BWI76_RS26100 0.79 -2.0 5,393,136 - BWI76_RS26100 0.82 -0.1 5,393,172 + BWI76_RS26100 0.85 +2.0 5,393,196 + BWI76_RS26100 0.87 +0.2 5,393,465 - BWI76_RS26105 0.49 -0.0 5,393,465 - BWI76_RS26105 0.49 -2.1 5,393,515 - BWI76_RS26105 0.61 +0.7 5,393,515 - BWI76_RS26105 0.61 -0.7 5,393,967 - BWI76_RS26110 0.69 -1.0 5,394,013 - BWI76_RS26110 0.79 -0.1 5,394,016 + BWI76_RS26110 0.80 +0.2 5,394,025 + BWI76_RS26110 0.82 +0.5 5,394,175 - BWI76_RS26115 0.15 +0.8 5,394,258 + BWI76_RS26115 0.31 +1.0 5,394,288 + BWI76_RS26115 0.36 +0.5 5,394,440 + BWI76_RS26115 0.65 +1.9 5,394,448 - BWI76_RS26115 0.66 +1.8 5,394,448 - BWI76_RS26115 0.66 -2.7 5,394,448 - BWI76_RS26115 0.66 -0.5 5,394,622 + -0.6 5,394,664 - +0.7 5,394,887 + BWI76_RS26120 0.33 -0.6 5,395,067 + BWI76_RS26120 0.56 -0.7 5,395,315 - BWI76_RS26120 0.87 -0.6 5,395,322 + BWI76_RS26120 0.88 +0.1 5,395,471 + +1.0 5,395,580 + +0.0 5,395,580 + -0.2 5,395,588 - -1.3
Or see this region's nucleotide sequence