Experiment: Fungal_growth_media_mCAFEs_v1 with Supernatant; Trichoderma harzianum M10 grown in Fungal_growth_media_mCAFEs_v2, filtered and pH-adjusted 0.8 x and biotin 0.32 ug/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS25245 and BWI76_RS25250 overlap by 4 nucleotides BWI76_RS25250 and BWI76_RS25255 overlap by 32 nucleotides BWI76_RS25255 and BWI76_RS25260 are separated by 6 nucleotides BWI76_RS25260 and BWI76_RS25265 are separated by 47 nucleotides
BWI76_RS25245: BWI76_RS25245 - 3-deoxy-D-manno-octulosonate 8-phosphate phosphatase, at 5,249,415 to 5,249,981
_RS25245
BWI76_RS25250: BWI76_RS25250 - LPS export ABC transporter periplasmic protein LptC, at 5,249,978 to 5,250,553
_RS25250
BWI76_RS25255: BWI76_RS25255 - lipopolysaccharide ABC transporter substrate-binding protein LptA, at 5,250,522 to 5,251,067
_RS25255
BWI76_RS25260: BWI76_RS25260 - ABC transporter ATP-binding protein, at 5,251,074 to 5,251,799
_RS25260
BWI76_RS25265: BWI76_RS25265 - RNA polymerase factor sigma-54, at 5,251,847 to 5,253,280
_RS25265
Position (kb) 5250
5251
5252 Strain fitness (log2 ratio) -1
0
1
2 at 5249.595 kb on + strand, within BWI76_RS25245 at 5249.720 kb on + strand, within BWI76_RS25245 at 5249.823 kb on + strand, within BWI76_RS25245 at 5249.945 kb on + strand at 5250.508 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Fungal_growth_media_mCAFEs_v1 with Supernatant; Trichoderma harzianum M10 grown in Fungal_growth_media_mCAFEs_v2, filtered and pH-adjusted 0.8 x and biotin 0.32 ug/ml remove 5,249,595 + BWI76_RS25245 0.32 -0.4 5,249,720 + BWI76_RS25245 0.54 -0.2 5,249,823 + BWI76_RS25245 0.72 +2.6 5,249,945 + -1.0 5,250,508 + +1.3
Or see this region's nucleotide sequence