Experiment: Fungal_growth_media_mCAFEs_v1 with Supernatant; Trichoderma harzianum M10 grown in Fungal_growth_media_mCAFEs_v2, filtered and pH-adjusted 0.8 x and biotin 0.32 ug/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS24225 and BWI76_RS24230 are separated by 181 nucleotides BWI76_RS24230 and BWI76_RS24235 are separated by 334 nucleotides BWI76_RS24235 and BWI76_RS24240 are separated by 117 nucleotides BWI76_RS24240 and BWI76_RS24245 overlap by 59 nucleotides
BWI76_RS24225: BWI76_RS24225 - hypothetical protein, at 5,049,806 to 5,050,144
_RS24225
BWI76_RS24230: BWI76_RS24230 - hypothetical protein, at 5,050,326 to 5,050,514
_RS24230
BWI76_RS24235: BWI76_RS24235 - Branched-chain-amino-acid transaminase (EC 2.6.1.42) <small>(from data)</small>, at 5,050,849 to 5,052,030
_RS24235
BWI76_RS24240: BWI76_RS24240 - hypothetical protein, at 5,052,148 to 5,052,354
_RS24240
BWI76_RS24245: BWI76_RS24245 - bifunctional glutathionylspermidine amidase/glutathionylspermidine synthase, at 5,052,296 to 5,054,167
_RS24245
Position (kb) 5050
5051
5052
5053 Strain fitness (log2 ratio) -3
-2
-1
0
1 at 5049.853 kb on - strand, within BWI76_RS24225 at 5050.011 kb on - strand, within BWI76_RS24225 at 5050.105 kb on - strand, within BWI76_RS24225 at 5050.107 kb on + strand, within BWI76_RS24225 at 5050.242 kb on + strand at 5050.630 kb on + strand at 5050.691 kb on + strand at 5050.691 kb on + strand at 5050.833 kb on + strand at 5051.030 kb on - strand, within BWI76_RS24235 at 5051.107 kb on + strand, within BWI76_RS24235 at 5051.542 kb on - strand, within BWI76_RS24235 at 5051.806 kb on - strand, within BWI76_RS24235 at 5051.860 kb on + strand, within BWI76_RS24235 at 5051.869 kb on + strand, within BWI76_RS24235 at 5051.869 kb on - strand, within BWI76_RS24235 at 5051.892 kb on - strand, within BWI76_RS24235 at 5051.947 kb on + strand at 5051.959 kb on - strand at 5051.970 kb on - strand at 5051.987 kb on + strand at 5052.194 kb on + strand, within BWI76_RS24240 at 5052.414 kb on + strand at 5052.453 kb on + strand at 5052.818 kb on - strand, within BWI76_RS24245 at 5052.977 kb on - strand, within BWI76_RS24245 at 5053.002 kb on + strand, within BWI76_RS24245 at 5053.021 kb on + strand, within BWI76_RS24245
Per-strain Table
Position Strand Gene LocusTag Fraction Fungal_growth_media_mCAFEs_v1 with Supernatant; Trichoderma harzianum M10 grown in Fungal_growth_media_mCAFEs_v2, filtered and pH-adjusted 0.8 x and biotin 0.32 ug/ml remove 5,049,853 - BWI76_RS24225 0.14 +0.6 5,050,011 - BWI76_RS24225 0.60 +1.1 5,050,105 - BWI76_RS24225 0.88 +0.4 5,050,107 + BWI76_RS24225 0.89 -0.7 5,050,242 + -0.2 5,050,630 + +1.0 5,050,691 + +0.7 5,050,691 + -0.0 5,050,833 + +1.3 5,051,030 - BWI76_RS24235 0.15 +1.1 5,051,107 + BWI76_RS24235 0.22 +0.5 5,051,542 - BWI76_RS24235 0.59 +0.5 5,051,806 - BWI76_RS24235 0.81 +0.7 5,051,860 + BWI76_RS24235 0.86 +0.2 5,051,869 + BWI76_RS24235 0.86 +0.5 5,051,869 - BWI76_RS24235 0.86 -3.7 5,051,892 - BWI76_RS24235 0.88 +1.1 5,051,947 + +0.4 5,051,959 - -0.7 5,051,970 - +0.2 5,051,987 + +0.2 5,052,194 + BWI76_RS24240 0.22 -1.0 5,052,414 + -0.5 5,052,453 + +0.2 5,052,818 - BWI76_RS24245 0.28 -0.0 5,052,977 - BWI76_RS24245 0.36 -0.3 5,053,002 + BWI76_RS24245 0.38 -1.4 5,053,021 + BWI76_RS24245 0.39 +0.9
Or see this region's nucleotide sequence